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Human element [ hs996 ]


Position: chr20:39,483,347-39,484,593 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MAFB - TOP1

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AGACCTGCCTAACCTTCCAGATgcgaaagggggatggcatctgcataatcttatttcatg
cttgtatcagccccaagtgataggggccataatattcccattttacaaatggagaaactg
agacctcagtctgtactctcattcggaatgtatgtaatcttcccagaactgtatcactca
tgagctgaaatttggatccaggtgcatgaagttccagagcctgtgctcctggccacctat
agtgtgagcccaatgaacctggcagccaggagggaattggcccaatgctgggctggggca
cgaaggtgactttcaataaatatttactgaatgagcagaggcagcagtgggattcacttc
ggggaaagttgaatccagcctgtgtgcagcggagctcaacccccgcctaccccaccccac
caccaccgccccacccccgctcacttatcaccatgtaattgaaataatgcctatcgacag
aaactcacaaacaactgctcaattttcctcaaaattcaattaaacttcgtttattctggg
gatcgatccaattaaaagaaggaaaatttcagatggtctgagcaacaccatccatcttca
aacacagtgtctaccccagtgatgtaataatttgcccaatcaatgctttaaaagcattta
gcagtcttgataatgaacttttgtcaaaatagccttgcagagcaataagtgcctgcagat
taatatgaagtcataaagggctaatgccttggggtggaagggggatgacgacatgaatca
gtgggagctgtgggctggggcatcatccttccacaccttctcagaactaggtatgagcag
ttagcaggacccagaaaatgctgaatgtacttccttgtttctgctctgtctgcaggttcc
tctcccctccacagtctcaagacccagactccaccagccaggtcctgtgatgctcagatc
accctcagggcatttcaggcaccccactgtattggagaaaatgtccaaagggaggaagct
ttgcttggaagaaagtctgcctcttggccatctaccatctctgaaagctctggcttcaaa
aatggtctttgagctgcaagaactttcttttctcttaccctttttcttggggtattcaac
ttactagctgtgtgaccttgagcatgttacttaaggtctctgaccttttgtttcttcatc
tgtaaaatggtgatgatagcatttgTTGAAGCTCAGAGGAGGGAGGT
Primer
(+)AGACCTGCCTAACCTTCCAGAT
(+)ACCTCCCTCCTCTGAGCTTCAA


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