Mouse element [ mm897 ]

Position: chr7:122,739,352-122,742,708 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: Sox6(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.

Fasta sequence

GTTCCCAAGACTGGGTCAGAtgaatgaagactattactgaattctggtgccagctcagca
tcaagatcaaacagccagcttagtttaaatcaatgttcaaaatggagctaccataaagaa
ctaggtcagagtgtgaacactgatgaacacatgttctcttatactgaatgtaaagaccaa
ctctatagagaggggtgacttctagaaacctcatgggaataaagatccaggacccttagt
ctaaaatgtggaaaccagaactacctcagattttagaatatttgcatagatttaccaatg
aagaaaaaaatgatatatagtgttttaaaagccagaacttttcaatgtcatcttgacacc
aactacttccactttcagaatactttcttttttgaaaccagaatgctcacccttgactcg
tttgtgtctgaagactttttaatcagccctcagagggacccatccttagaatcagtatcc
ctggatcatctgagtctcccccagattccttctctcataggaaattgagttccctaccaa
acacagagaggcatctatggtcagaagccgttttaattgtaagtttgcttttgggctgtt
tcatcagtttccttagaatctcagtgaggtggtttatatctgtggcttaagcatattaaa
atgcctttgttcatgttctaagccacacatataaaggaactgctgccctcagattggctg
taccctgactaggtagttcatataatcaggctaatatgttaaaaggaacttgacacagga
attattaatgagcaaaacaaggtcactacctctgagctggggtcacttacaaagaacgca
caaatgaaatcgaggttggcttctgtgtacttggctattttcttacgttggattcctttg
gcaacctatctttaaacatttaaagccatgtttacaaggaggctctgaaagaacttcaaa
ccattatgcccctaattgaattccccaggagtccaggaaactcagaggaaaaaagaaaaa
tcatagcatttgctaaatcatctcaaagcacatttcactacaaggttatatgacctgatt
ttttttttttttgagttcaaggatgaatgaagactattactgaatgcttcattatttaga
ccaaaataaaaaagaactttacccatcagtggtgaaataattaaatcagcagttttaact
ttttttcccaaaggacattttccaaaaaaggaaaaaaaaaaaaaaaaaaagaaaaggaaa
gaaagaaaagaaagaaaaaagaataaaagaaaagaaaaggaaaaaaaaaacgtaaacaga
acccaaaggcagcatgaccagtttcccagtgtgaaagctcaagatgggcaaaagacctgt
ggacaaccaaaaatggttaagggtaatggagtgagaaccatgtgggacttttatatgtga
gttcgaaataaactctaatgagaaaagagagaagtaaaagttctagagaccgattagtta
tgacaatgcccccccagctctggttttactttgaaataggcttttatggtgtttttatcc
atagctcccttgtctgtgcctgtgtttgttgtgactactaagcaaaaacatgtgggcccg
agcatttccaaaaattgtacccgcggctctccacttacctggcttgtatgttattccagg
ggggaagaggaatccctgttgggcagcagcagctgctgccaaggtccgctggtcatgtgg
aaaaattgggatcatgagcggaggcatgtgcccctgaacctgctaaacagaagagagcct
gtgatcagacagggagcagatgttaccaagaagcacttgtctcccattgctggagctgct
ctgctgcccacacagccgcccacttcaaacctctttgcagagctgaagctgagaacactc
aggcaaactccacctaactggtctccttagctgcctgccgtgagcagaccaccgtcttac
cagagactgaccacatccctgctttcatctcaaggtggtcagtggccttcttctctcttg
acttgtttttctagacttcttttgtgaaagaaagagcaggaaaactaaatgctctgcttt
cctctcaccctgtaggtgagaggttctgccctttcccctgcactacattctaagcttcac
tgtgaaaacagtatttagcatccgtggccagaaagtaatgtctaattgttatatattcca
gaagctgtgtgtctccctcatccatgagcttcttccaaagcctcactgcattatattaaa
cacacagcgcttcagatttaaggctactatatcttggcgcccaattaaactaatatcctt
tgccatacacacctcgctgtaacatataaattgcttgtcagctttatgcactgccatgta
gctcagtaattatggagttgtatccactgagcaactcataataaatcctactatatcaga
tttcatttgacctcaataaacgtatcaaagaccacaaaaatgcaaaagagaaaaacattc
agtctacatcagtatttgtgctctatataaaggaaatcagagctatgacagaatcatgct
cctgaaaatcacattttaatttcctacctcccttcttttctctagcaagctgccctggtt
ccagcagagcagagcagggaagctttgtcctggcctttaaggcctgagtccataggcgga
gccacgggactttcttcatttaaagatcagaacagagatgcgtcaaaaggcacagtcttt
tggtttagacagatctaaggctttcggtgacacaaaatgactacattatacacttaacta
aacattaagatagaaatgtgttccagcagttctacaaactccaaatgcatcctctaaatt
cctaaatcttttctttagatgaaatcccatatatgagctgatgcccctgtctaaatggta
ccagcttactttctcctgtctctgtcccttcacagccagggacactgtgtaaatgcttct
gtgtgggtctgagaggttccatttgccagcagtctttctcgggggaatgccctaagatgt
gggttaataaagggcaagcaaaaaataaacactttacagtattttttttctgaattcatt
tctcaggaaaatcaagttataataaaaaggtctttgtgcaattactaccttgccagtggg
gcgtgaaagaaagtagctttgagcagctatttcttctattttagctgaggtaagcccaag
ctttgtacatgagagaaccaactttgcctggttaaattggagctgaacctctagtgcccg
tgcctcacctttagcctggaggaaaaataccagGTTTGCTGTTCAACCCCAGTttgc

Primer

(+)GTTCCCAAGACTGGGTCAGA

(+)caccACTGGGGTTGAACAGCAAAC