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Human element [ hs827 ]


Position: chr7:69,881,761-69,882,691 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: AUTS2(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
gcCCTGTCTTGATTCCCAGGTGCTgggaagacatgattgcagcctcctgcacccctgctc
ccctcctctgacacacacactctgagcctgcaaggtacagcagcccactaaagctctagt
taaaagtaccaagttgctggaatgcccagggaagtgagaatgagcgggtctcacacaggg
atgatttgaaacaagccttgtggctttttaataatgtgccaaagcggtctcagtggtggg
ccagcagggcccccagtctatcttctgtgaaaggagaaagcccagcaggccctgccgaat
gcagacagcccctccactctgctggaggagacagactccgagatggcaatcaaggcttga
tttgccaacacatttccaatgattaaaatgtaattagcacaactgagtgcctcctcccca
gctaattttaatacatgctgtgacaggcaggcagccggctgtgcctttagcccagcagca
gatggaggcagaggcagcgggagaggaaaaccattttgcaatgtgactattcgcatcaca
aaaccatcagggcgctcagctcttagaaggcccttgatggcagcacatcaaattggcgct
gtcatagctgaccgtggcaatggcagtcatccttcatactgtcccagaagcacagcgagg
caatttccaggcgtaaccctttaagatctagaaggggagagagagacttggagagcacac
ttgtcgtcttgcagtgacaggcgagagctttgcaatgtgcgctgtgaaacccacgctctt
ggcagcttgcaaaccgttacttacatgttccttttccaaggcacagtggagtgtggttca
gaagctttggaaatagctgtttctccaaatgggagacaggcgtgagctgccttctgtgtg
cattatctgAACTGGGAAGGATGAGCTTTTC
Primer
(+)caccTGTCTTGATTCCCAGGTGCT
(+)GAAAAGCTCATCCTTCCCAGTT


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