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Mouse element [ mm767 ]


Position: chr10:30,747,625-30,748,534 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: Hey2 - Hddc2

Timepoints:

Published in: Dynamic GATA4 enhancers shape the chromatin landscape central to heart development and disease. He A, Gu F, Hu Y, Ma Q, Yi Ye L, Akiyama JA, Visel A, Pennacchio LA, Pu WT. Nat Commun. 2014 Sep 24;5:4907. doi: 10.1038/ncomms5907. PMID: 25249388

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
tgaCACCACCACCATTTGTGTTAttgaaggcagctgaggcagggatcactggctggtttc
attttagctacccacccagatctacttcaacagagcttgtagaggtagtagtggttgatg
tggcttgctaagacacccatcctcctttctctgcccaccccagttcacccagctccatca
acgtcctcagatggagagcaaagaaaagctgtaaggaaagctagggctttgatcttctac
agaaatgggcccctttctctgtacacatgactaggggtcactgaggagctgaccccagtc
aggatccacaggtctagtgacctgctttgaagagtccctcaaagccaaggacaatgaaag
ggatctgttcactatgaggatgtctggttgcaatagccctcgaccctgttctaatgactt
ctgtcttgggaggacaagataatcctgcctttgagccaactttatgagctccttgctctg
cacatcttctcctctgacttggggtctctgtagggatgagggctgtatctgctttcattc
ttaactctgttttattccccatgcagaaaaagataagcctccttgtgggaaagcagctgc
tttggatcaggttagaagcagtaaatctggaaacaaacataacatgaatgaaacggtatc
aggacatgacgtcatgatgatgaaagcccaggcgtttggctcttgcacagtcagcctgct
ctaatctttcattcacacagacttcacactgttctgtaattaaaaattttcatcgcctga
tttgataattcacacaagatcctttcatcaatgccacatacaattttgatcatcttcaaa
ttctcatgggtaattgtcttgatttataacaatttaaataaaactaTGTTGTTCTCCACA
GCTAATTTTT
Primer
(+)cacCACCACCACCATTTGTGTTA
(+)AAAAATTAGCTGTGGAGAACAACA


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