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Human element [ hs520 ]


Position: chr10:102,551,472-102,552,372 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: PAX2(intragenic)

Timepoints:
e11.5

Nonconserved element tested as a negative control.

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
GGGATGGGGACAGTAGGTTTggtaggagtgactccaggagccgttctttcctcctttctt
ccctcctcacctgccatctccatctctttaggcagagtgcccagcatgagcccttgggca
gtgtgggcacctgggtggactggtggtgtggggagccgagggcactgtgggccacctggg
gcagccagcttgtaggagccgcctgggtgagggacagtgtgggtggtcttgggcagtggg
tatgtggtccaggcagcaaaagggaccagaatgctatacaaaggaacctctagggacttg
ctttttcttcggatgctggggatccatttttctctggcattttggtctgaaccatgtggg
tgccttacagccactgaagataattattggagtcttttaaggagaaatagagaccgtatt
ttttttcctggtaattcaaaggaaataagtggttgacaatgttaaagtatgaaagctcac
agagggacttaaaagcaagttggaatattggagtatctgttcttctggactcttcttcac
gtgcacaagcacatgggttcgtgcgtgtgtgcactaataatgtgtaagtgggccaggacc
cttgactttagatgaaaggggagtgtgggggagaaaatgcctggagaggaagtccgcagc
ataggcctttgctctggcctgtgggcttggcaccatcatgttgcttgcccctatggcttc
tgcgacatacctcagctggggacagacagtgagaagtctatctcccttctctttctggga
actccatgtctgctctattcaaattctaccaggaactgagacagaagaggatgacagagg
tagaaccatccagaagcagaggatggggagaagagggcaaaAGGATAGCGAAGGATGCTC
A
Primer
(+)GGGATGGGGACAGTAGGTTT
(+)TGAGCATCCTTCGCTATCCT


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