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Human element [ hs518 ]


Position: chr11:16,327,942-16,328,841 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MIR6073 - C11orf58

Timepoints:
e11.5

Nonconserved element tested for negative control.

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
GACATTCACAAGTGCCAGTCAgtagaagattaggtatgatccggaaaatggataaatgga
aaatttcaataaacggagagaatacaaagttattctggataggagggataatgactaagc
ttgaagtatgttggactaaataactaatcaacttcagcactgtgctgactcaacagataa
aaatcttgtcctgagattgttacatcctcaaatttctttttttttttttttctttttttt
tttttcctgagacagattctcgctctgtcacccaggctagagtgcagtggcacgatcttg
gttcactgcagcctccacctccccagttcaagcaattctcctgcctcagcctcctgagta
gctgagactacaggtacatgccaccatgcccggctaatttttgtatatttagtagagacg
gggtttcaccatgttggtcaggatggtctcgatctcctgaccgcgtgatccacccgcctc
agcctcccaaagtgctgggcttacaggcatgagccactacgctcagcctacatcctcgaa
tttcttaatcacaatgctcactagagaaagattacaacagaactccaggaaccaaattct
aattatgtgcagtgtgtctaatataactaaggaatgtccaagttatatgtttgcgcaagt
gcaaaggtcctaagacatgtttaaggaggaggggagtcgtccatggcagagaagagtaaa
gtaatataaaatggggcttgaaagccagagtaatatgttaggattttaagcaatgaatgt
tattctaggcataatagaaattgatatcattagccagataccaatgctccaatgccaata
aaatgcctaaactctctggatattagataacaccaataaGAAGCAAAAGGGGCATAATTC
Primer
(+)GACATTCACAAGTGCCAGTCA
(+)GAATTATGCCCCTTTTGCTTC


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