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Human element [ hs477 ]


Position: chr3:168,833,392-168,834,540 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MECOM(intragenic)

Timepoints:
e11.5

This represents an exonic ultraconserved element.

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TGGAGTCAAGGGCTTCTCATcctttcgcttagtggtgagatcaaagggggactcagagct
gcccttctgcagtttctttacttcacctggtgattggggttccattttcaaaggtaacga
tctcaagtctctatcaggaaatgggtacattgattgagagaatgctggaaaaaatgggag
gggaaacatggaagggtaaggtaaagctccaacttttttgtcttgcagccccaccagtcc
tgttgaaccaaagtatttttcagcaatagaagcaatagcctttatagaatcattcacagc
tcctgacaccgcagtctgctcctctaaagatggtgagaaaatggaatgattgctgtattc
tttcttattatttattgaagccagattctgaagagggcttactttgtctttgaacatttt
accattttctttaaatttctctttatcactttcaatgtcactttccagatcagagcccga
ggttgtttccaggtcactgccacttggtgtactgacatcatcaaggtcactactctctga
ctggtcactgattttctcaaagggcctctcttcagaggacctctcgggctggagctccac
tggcttattgtcccctacagatgggtgtttagatagtgccttcaaaatatcctgtgtagc
tggcagtatctgaggatgtgtcatgaggggactttgacttttgtttgtctgttcagtact
tgatagtcctttaacaggagaactagcaggtatcaaaggaggcctgtggtacaagccgga
aggaaacagaccagggaagctaaaagaaaatccaggagctgttggaaaggtaagaccagc
aggatgcctattggcgccaaaatagtcagcaaggcccgggttggcatgactcatattaac
catggacgttttatccatagctggggttccaggaagtgaaatgccttggccaaaaaatcc
acctgccgcaaaatggttcttgccctcacaaaacctcctgtgtttatttaaggaagacgt
agtgctgaacatttgtccacagtctttgcacttgatttgggttctgcaatcagcatgcat
gcgcttatgacggcaaaggtttgaaaactgagtataggatttatggcagACCTCACCTGT
GTGCAAACA
Primer
(+)TGGAGTCAAGGGCTTCTCAT
(+)TGTTTGCACACAGGTGAGGT


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