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Human element [ hs473 ]


Position: chr7:35,342,478-35,343,110 (UCSC browser)
Source: University of Chicago - Nobrega Lab

Flanking genes: TBX20 - LOC401324

Timepoints:
e11.5

Data entered by Marcelo Nobrega (nobrega@uchicago.edu).

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TAGGAGAACTAGGTCCTTCCTGAGCccagtacacctgcgggaagcacccc
agaagcctgccccatcctgtggcttgaaggcatctccaagcaccattcag
tggctggtttccctaagatgcaactttggggcagtctgcctgtgagagta
gataagaagtgagcttagcagcatcttttatcagctgcttatgctcacag
aggacagatgatcttcaaggagttgcttagagatttaaagcactgatgtg
acaaggatgctttgattccaacgtctcagccctctccctgcagccagtgt
ttacataaacttgaaaaaacctgccactactgttcatcgccctcgttttt
caaccttaatggtaattacattccaaggactaggagagattttccctctc
cttcaagtctggggttagatattccctttgacatggcgttggagctttgg
aacccggtgctgggggcaggggtggggtgagttgaaggagacactgcttt
ctgatagggtttttaaatcaattcttagaaagctttagcttgtatattta
ttgtttacttttttattattgtgtaagcagagttttgacttaaacttgaa
atttatttTTGCTTGAATGTATCAAAACTTTGC
Primer
(+)TAGGAGAACTAGGTCCTTCCTGAGC
(+)GCAAAGTTTTGATACATTCAAGCAA


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