Mouse element [ mm374 ]Position: chr4:89,072,503-89,077,616 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Cdkn2b - Dmrta1 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GAGCTAAACTGGGGCATCACtttcctctccatgactgctttgaatagacataagttttcc tgatacagtcttccacgaggctccaagaatgaggttaaactctataaaaagaaaattgac caacccttaaagccatccttgtgaactttaaacaccactgaatgtacatagtaaaattgt ccctgggcacacagactcccaggcttgggtggagttcacatcggctgttttgctaaaata aacaaaaatgactgagaactttggattgcctttgtctgaacttgagtgtctggatggatc agcaacagtagtgtataacatacctgcttgtcaaaccaaggagaaacccaataggcatgt gtttcttacaagtcaacaaaaataatcacacgattgtaatttgtggtagccatttgttct aaaaagccacctcacacattttccagccaaataagtcttcctttgaaaggcatgggatga agaacaacattaagtagaccattttgatctctaatttcaaaagatgacattttaaaattc aactgtaaatctaatgagacacactacaacatccagaacacatttgagttggtttatgaa actaaaaacaaaaacaatgaatttcttaagtttttaacttaaaaattattattatattga gacaacagttaacattaaacatttatagatggctagatccaaacttgaagggaacatttg agttaaaagtagttagcaaacacactatatgtatgtatcaaattctgaaacatggaaaaa gtagttgaaaaaatcaggcagattgtgtaaatgcaatatgaatttatacacacccaattt taggaaagctacaggaaggtcccatgcgcattctcttgagtgacgcccttgctttatatt aacaacactgaccatctgacttcagtatcctctttactcacaacttggtcaaagacatct cataattactaagtgcctcagaaacgatttctaaggctagtagcatgaatttattatgtg ctttactgggttttgagcatcattgatctctgggttttttagatctgatctcttagtgat accatgaattcatagtagccacaagtgagatgtgtaaatgtcattttcttatgtttgaaa tttgctttttcaaaagcaaacaaaaatcgactgaatttagtcaatattggtcatgttata gtgatttgagcacataatttagattgatgtcaattaaatgatgtctgtagtctataaaaa cgcgaactcttatagctgcttcacttaaggagtggttcctctgtgtatacaatttctcta agaatgttggagatattaattagttgaactttgtgaaatgcattagggcattcttttttt ctctctctttttttaattaggtattttcctcgtttacattttcaatgctatcccaaaagt cccccatacccacgccccaatcccctacccacccacttcccctttttggccctggggttc ccctgtacaggggcatataaagtttgcaagtccaatgggcctctctttgcagtgatggcc gactaggccatcttttgatacatatgcagctagagacaagagctcctgggtactggttag ttcatattgttgttccacctatagggttgcagttccctttagctccttgggtaatttctc tagcttctccattgggggccgtgtgacccatccaatagctgactgtgactatccacttct gtgtttgctaggcctcagcatagtctcacaagagacagctatatctgggtcctttcagca aaatcttgctagtgtatgcaatggtgtcagcatttggaagctgattatggggtggatccc tgcatatgacaatcactagatggtccatcctttcgtcacagttccaaattttgtctctgt aactccttccatgggtgttttgttcccatttctaagaagggacaaagtgtccacactttg gtcttcgttcttcttgaatttcatgcgtttagcaaattgtatcttatatcttgggtatcc taagtttctgggctaatatccacttatcagtgagtacatattgtgtgagttcctttgtga ttgggttacctcactcaggatgatgccctccaggtccatccatttgcctaggaatttcat aaattcatttcttttaatagctgagtagtactccattgtgtaaatgtaccacgttttctg tatccattcctctgttgaggggcatctgggttctttccagcttctggctattataaataa ggctgctatgaacatagtggaacatgtgtccttcttaccggttgggacatcttctggata tatgcccaggagaggtattgcgggatcctccagtagtactatgtccaattttctgaggaa ccaccagactgatttccagagtggttgtacaagcttgcaatcccaccaacaatggaggag tgttcctctttctccatatcctcgccagcatctgctgtcacctgaatttttgatcttagc cattctgactggagtgaagtggaatctcagggttgttttgatttgcatttccctgatgat taaggatgttgaacatttttccaggtgcttctctgccgttcagtattcctcaggtgagaa ttctttgttcaattctgagccccatttttaatggggttatttgattttctgaagtccacc ttcttgagttctttatatatattggatattagtcccctatccgatttgggataggtaaag atcctttcccaatctgttggtggtctttttgtcttattgacggtgtcttttgccttgcag aagctttgaaattttatgaggtcccatttgtcaattctcgatcttacagcacaagccatt gctgttctattcaggaatttttcccctatacccatatctttgaggcatttccccactttc tcctctataagtttcagtgtctctggttttatgtggagttccttaatccacttagatttg accttagtacaaggagatagaaatggatcaattcgcatttttctacgtgataactgccag ttgtgccagcaccattcgttgaaaatgctgtctttctttaagggatttttgtgtttcctc tttaatgtcttctacttgtttagcagtgttctcctgtatttctttaagtgagttatttag gtccttcttgatgtcctgtaccatcatcatgagatatgcttttaaatccaggtctagctt ttcaggtgtgttggggtgccctggactgggcaaagtgggagtgctgggttctgatgatgg tgagtggtcctggtttctgttagtaggattcttacgtttacctttcgccatctggtaatc tctggagttagttgttatagttgtctttgtttagagattgttcctctggtgattttgtta cctctatcagcagacctgggagactagctctctcctctgagtttcagtggtcagagcagt ctctgcaggcaaggtctcctctttcagggaaggtgtacagttatctggtgtttggacctc ctcctggccgaagatgaaggcccaaaacaggatctttctcagaagctgtgttgctttggc ctgtcacggaagccgttgtttcagtagtccacactctcacctgtgtagactactttccgc agagtcccggaaccaaggtggctcccgcggaacttgaggcagaagcctcccgggccgggc ggacacctgtgctctgaccaggaaggtggcccgttgtctggcgccgaaaatggcgccggc attagggcattcttgatgaatgtttggggatgtagttttaaacaaacaaaaaaaaaagat taactctttctgccttatagaaaacataaattcattttctctaatttagtggtcaaaatt ttttgatgttaccatgcatacagagtgttttggtggttgggatttaagaatagatcacat ggagtagtaagcataaacaggaaacttttcttaagaagactacacatcactgagatcaat attcttatacacacacacgcacacaaacacacacatatacagatctctatgtatatgtgc acatatgtaattcaaaggagtatcttttattgttgattttattctcaatgattagactgt ataccatttaagtaatgctttgtgttggtgagcaatttgaaatatttcttccttgatatc ctttttttcatgaggttctttttttttcatgagcatatcataactataatttataattac attccatttttattactgataaaatcacaaatcagtaagttatttttctttgaaaatatt agttttgaaaatttttagatagtataaattctacactgtaaaatgtacatcattctggcg acttcttgacatttttctgtttgacagagtttgattaacttatacccagtcattgtgctt tgaatgagaaatgtccctgagaggttcatgtatttgaacacgtggtcaacagttggtggt ggcactgtttggggagattatagaagctttagtaagtacagcctcactggaggaagtacg tcactatggggtgggcttaaaggatgtatagccttgcctcacctcctcctccccatctct gctttctagatgcaatcactggtgctggaaaatataaacatgggcaagtctaaaaattct tgtttttaaatatttgtaggaattgatataatgtatcttcatatgtgtgtttgtgtgtgt gtctgtgtagccagtagcaagatggctctaccatttttcttgctttacttttgctcattt ggcaggtggtttttttatggccatgcttagtcagaatctcaaattctgtcaagttctata atgttcttatagagagaaatcacatcatacaccctggactgtaaaacaagatatatccta aactctgttccatgagactcattaattcttaagtatgtttaacttaaaggtttgaataca atagaaatgggtgcatttaaaaaacaacttaccagaattccccctcctcccaaaaaataa acttcaaaaatatgaagaaatggttttggttgaactatttgaagaacaaaggtgTTTTTA CACCTCCCCCTTCCPrimer
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