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Human element [ hs354 ]


Position: chr15:35,918,719-35,919,621 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: DPH6-AS1(intragenic)

Timepoints:
e11.5
e14.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
ACAAAATGTGCCTCCCTGAGcatagcacacatcctcaaagaatgctaacactttacaaat
ttgctagctaatgtgtaatctgcagaggatatctgccttatctgacaaacatcatctcag
ctcaggaagccatgtacaatccgtctatcagatctgacattggtggtatctacgcagccc
ttcccagcacacaccctgcctttaaaggtcttcccatccctgttattggtctcacagatg
atagagtatgacactccataaacaatgagctaggccttttgtaggctgctttgcttttct
tggcttcaaactgccactgaatgtcaaatcaatttggctctcattagaacagctttatat
gtgcaaatgtatttgggtctggttgccactttttcccctatttttttctcctttccattc
ctcctcttctctttgagatgatgtaatttttctctaatccatgccaacacctgtttcttt
aaaagagagagagaggagagaaaaaagaagagaggagagtgagagaaaatgctcgagtgt
gtggctctttatcacaatcagcatgaacaccagtctggcacattatggctcaagtttccc
cagaacaaagagaaaatgcctcgttgccatgatattgtacatcacagagcccagataaag
tcgagagagtctttgtaaaccttggttcctgatgtctggagaaacggctgtcgagataga
gcaaggtgtgtaagctggttttattgataagtgatgaagctagcaataaatctggtgcag
tgcttaaggaagtctgagccacagaccatgtttgatgggagactgttttcctcttgagtt
gggagaaataaagcgctacacctaagagaatagcacagacttaAAGAGGCAGCAGGAAAC
TCA
Primer
(+)ACAAAATGTGCCTCCCTGAG
(+)TGAGTTTCCTGCTGCCTCTT


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