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Human element [ hs351 ]


Position: chr11:61,311,775-61,314,751 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: SYT7(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
ATGAgAGCACAGTGCAGCAGaaatggagctcctaccctcccaaggagtttattctaaaca
tttcaccctacgccccttatggcgacccacgactgtccctcaagtgagtgactttacctg
gtttgatcatatgtaccgagtatccgcgcacgccctccttccctcctcctgcccccaccc
acctctggacccgctcactccctctctcctctcgctcctgtcccccaacttccccaccca
tccaccccatgggcaggccgaccatggagacgagcaaccccacctgccccaacccacctg
cctgcccgctcccgtcccctcccccagggccagccttgcaggagagccccctcggcagcc
atacctggcccccccagctggtcccggggcccaccccaccttctgtgcagtcaggatggg
gttgcccgtttgggtcccgccccctcggttgtggtcgcttcttcctccccgctgtgtgct
gccgtggctgctcggtggtcggtggtcggtggtcggtggttcgtggcggtggtggcggtg
atggtggtggtggtggtgatgaactctgactaacacggctttctctttctccctgccttg
gggcctcctggcctggacagcccgctcttcctccgtcgttaacccttcgttgtcctgtgg
gatagagttggaggtggctgccctcccccaacccccgccgcccctgccccaggcggtggg
gaggggccccccctccgttgtcgtggtgcgttgttctccgacccccagcccgcccggtcc
ccctctctcctctgcaggctccgctgttaacccatttgcagtgctgatgtctctctctct
ctctctgtctgtctcatgtccgtctgtctctctcctcctcctctctcactgtctctttct
tccttttatctgttattgtttttcttcctcttccaccccccgacccccacgtatggcctg
ccctctctcccccaccgccacccccggtgcccctctggcctggctgcctcgtgcatgcct
ccagtggcaccctcctgtcgggcgccaaagtggccgccgcggcggggctggcggtggagc
gggaaggccggctgggggagaagccggcaccggtgccgccacccggagaggacgccttga
gaagcggcggggctgcccccagcgagccgggcagcggtggcaaggcggggagaggccgct
ggcggacggtgcagagccacctggccgcagggaagctcaacttgtccaagtgagtgatgg
cgccccatggctgccggagccatgagctcaggcctgccctgccagggtggacagcgggac
ccctggcatgcccgctgctgcccccagaggtgccgtcctcggcccctggctcattccatc
ccttcatgagactctcccatcatccttgccatctggcacgacgaactcagccatacagtg
atgcatttcagcctctggctgatgccccatcagtcccttcctttgccagtgaccaggtca
ttccattttctgcccccagggcagcttcatttcagtccctgggtcatcccatcctctttg
attggcccccccatctcagccaccaggtcatccctccatgggcagcaggcctgtggttgt
tccatctcatcctgggtatctggcccactctcagcctgacaatcacccatcagctcccag
gctttccctcttacagccagcagatggctctagacccagaagttgagccattccattttt
gaccatcgggtgactccattggctgtcccagtcttgcttgaaccatacatcatctgttgt
ggaagccactcacgagcagccgcaggctgccccatcggttcatcctcatctcagccatcc
ccgttggctgtcccagtcttggtcaccgagctatcccaccactggctgttgggaccgtgt
attcctggaggttgggttgtcaaaccccagctagcttcacttgccagatgtcatggggtg
ttctagacatcaggcagaaggagccgtcccctctgtaatagctgtctgagcttgtcgaac
agtcaggcccactcttggctgatggacgtaggattggcccctgttttccacagacagtat
atcccctctcatggggcagctttattctcatcttggcctttggaaacttctgattcccct
catcagagcccttctaggccctgagtcctttgagctgtgaccagtctcccttggggtata
aattccattttcagaagttggaactcccagctaatggagcagcctcccatttaacaggcc
aacagccctgccccttcctagctgttgggccatcctgttgcctcaactgagggtcctcct
gattctcttcagaagacctgtggttcctgccattgggagcaccgaaggccaccgtaccaa
gatctctttcgctagttggcttgagtgcacacaagccaatttctcccaacatctggcccc
aggagggtcccacagggaagccacacaccccttcagagagcccggcccagtgccccaccc
tcccagcctgccccggccagtctccaggccggttaggcctccatgccatccactggctcc
aggcctggaggaaccaattttagtttcctgtgactgagaggttgtaaattggactaattt
tggatgtgataggaaaccaaaatgaggtcaccaaggagggggaggaggcagctgccaatg
tgccgccctccctctgccttgacctgcccccagggagctgtgggcctacctggccggcca
ggcctgggccggcccctggaggtagcctggatttcaggcctgagttaggcaaggctgcca
cccctccaagaccccagctaaagctacgttttgctttcttctctttctttccctctctct
ttactatactctttctctccctctttcactctctctttttctttctctctctcagcccac
aggggtagcctaaCTGTTGTCTTTTGGTTCCCAGGgt
Primer
(+)ATGAAAGCACAGTGCAGCAG
(+)CACCTGGGAACCAAAAGACAACAG


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