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Human element [ hs328 ]


Position: chr10:114,403,685-114,404,566 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MIR4295 - TCF7L2

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AGTGAATGGCCAGAAACACCgtatattttatgaaatatggcagcataatgaataggaggt
aaaaaaaaaaaaatgtcctggctttcaagtcctgatggttgtgatgtgattgaatcatag
tgcattctgtttgatgtctcggctttgctattcagatgcactgcaaagtatgtgcatcac
cctctctgcaaagtgcacctggcaacccagcacggatgtagtttgttttcattgcatctg
caagaaacatatatattagagtagaatttaagtaggagtattttaattcttctaattgtg
atagtttccccacacagagaaaactatcaatcaggtacaaaagaaactgcttaggatttg
aaggaatttgacaattttgttgtagttcctagcctggcaggtggtaataatgcctatgcc
agcactgtcaaaatatgaaaattaaagtgacgatgggttttaatgaaatccatttgacaa
atataattaaggtgttttaatatgaagtcctgtaataaagactaccggtggactccctga
ggtcctgactaattgtgtagattgcttttaagctttgaatagttttattggataatgaac
tgttaagaagtgttagatctttcttaagcagaatttggtttgaaaaagaagaggagagac
aggcctgaggtgtggcattttaaacataattagaggagagatttaagttaacatctttga
tcttgctgtcagtttttgattagagctgtaaatgctttaatcaagtgtagtgtagtcatt
acatttctaactgttttttaaactgcgaaattactgagacattgacatcaattttaacac
attctgaaaatgtacagcagaATTGGGCTTCTCTACCTAGCG
Primer
(+)AGTGAATGGCCAGAAACACC
(+)CGCTAGGTAGAGAAGCCCAAT


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