Human element [ hs2749 ]Position: chr1:170,773,786-170,778,693 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: PRRX1 - MROH9 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GTGGAACCCTTTGGCAGTTAgctgatatctgctgcagataatagcatactgacaaaaagt cccttaaatcctaaagcatatgttttttaacatggcagggaatctggaaggatggctgtt cagcagcacagtgatatcctcaagaactatggatttccacctttctactctgccctatgt gttgctaatgtttcatgttcaagagcatagtgctagcggtttcgggcatcctaggaagcc atgactacgtttatttaagaggagggagaatgttctttataaatctcttttttaatttgt gagaaaatctttcctccagtgcccccagtaggattctctgcatgtgctattggctagtaa taagccacacatctaggcctaaatccaatcacgattcactctctgagattgttcaagacg tcgatttcccttagccagcaaaaaaagaaggtacactgggagagggctggtgatgctgtt ggtatggcaaaaagaaaaaaaaaattgtctgccacaagcactacaacaaacttcttgttc tagggattaatttagttttaatatggctgatggaaatggaggttgggagaagagaggagt acagacaagaggggagaagagaaagggaaaatgtattgagcacttccatgaattaatgtt agctccttttacatacttcacaatatgctcttcacatggtacttttattatacccatttt ctagaagctcttttaagtctttcaaatcacacttgaaaatggcagtgacgatttgaattc aaattgatgattagaaggtacatgctctctagagatactggtttgttttatacttgcatc taaggtggataacttctccccttaggggagaatggctggaagttatccaccttagatgca agtataaaacagaccaatatcgaagaataactcccatcttttggaaaacttgtctaatgc ctctttgcacctttaaccttcttaacaccccgaattaagctaagtcattcatccattagt tgtatgcacagcctgttgtgcagtcgtctatgtttttacttcttggtattctctgagtaa gaggaaacgtctgtgaccttccactggatccttgcttccatatatcagaggctaaggcaa tcacagacagcactggtgggaaaccagaacagaaaacacacacacacacatgcacacaca cacacacacacacacacacacacacacacgaatggaacgaggaactccaagagggacttc taatatttcattgttattgaaaattacacttcataaaaagtcttgttcatttaaagtttg aagagtgacagaaaaatagctttcatactcaagtctgctaaatgtatgataaatcaaaaa agatggaacaaggagaaacttcaagaaaaacaaaacagaaaatcagtgtgtagtataaat aacacttcattagtgctgtaatgccttacaaaacagtctaaaaaattccacagggtaatc gtagtgtacagcgcagaagagaataatttactatcgttaggcgtttgtaatccctacaat ggaaaggagaatgtatgcatgtaaccctttaatgatagtggacttgcaggcataaatccc taaatgatgagatgtgaatacattttataaattcaagtttctgttggggaagaaaagtca ggctgtctggtttgactggcatgctgacctaagtcaattactgaaccacggagagctgta gccttgggagtgtgctatgttctttctacggtcttaagagaggccaggctcaggggttga gcctagcgggctggtctctcgctcatcaggcacacatcaacactataaacacacagtttt tagctagtggcagtcaatgccaaaggcctggcagcatgccacaccatctcaaggcactct cagcaataactcaaatgtttttctgtcagaaaaactttttctgtactgttttcaacactt cagcttagtgtgggaccgaagcagaacgagggataggaaaaattaataattttacactcc agctttcaaagccccctttcctttggtagcagagtttcttcttttcattctatttaattc tcaaactctcctcaacaaaatcttctctgaagcaaagacctaaaacttattaacacggga atccaaatgaaagacaataggttaattgttacttctcccacaggtgttttaaaaaatgtt aatgtggattcaatccttaaactaagcaaaatttagaaaaagttttcaaaggagtgttct ctggacaaggatgcctggatgttggatgggaagtagggagttcccagaggctagattgga tattatggaaggattactagagtttaaccacggaactgtaagaattatgatcaggcagag aacaaagcagtagatcaaatctaaaagggagacaacataaatatgaagaaataagatttc aggttagacgccaggaagaaggggcccaggttggaaagcagaagcatgagacgagagttc caggaccagtgattcaggggaggctgtgaacaatacagagagggccagaaacctgttgtt acatcatgttggttacatgggacaagctaggttaaaacaggagttcttcagctggtggga ggggtggtctgagagagagaacagtgatccaaggacagccttcaagaggtctggaaaaac cctgaactctcctttaaaatgtgcaagaagaaaacttgaagctttgattaaattctcaaa gcaatatgtcaccctagaatgattaaatatagcagcccaatgaaaagcacctttgtggac cctgtggatcccgtggcagtggcggtcatctactgtcagtcatgtagttcagcctggttc ccacactctgattccatcttctttaagcacttgcagcaggattggtaatcattcgcattc ctttcttccagttagtacccctaaaggaggaaaaaatggttttgcttaacatggactatt atgaaatggcatctctctctctggtgactcacaggaatgaggcgcccttctctggaaggg taggttgcccacaataatattgtctttgttcttctagatgaacagctagtccatctcagt ctcttgccttatagatctctcaggtatgagccaaatgccagtccactagatatcctgggc tccaagggccacatatcaaactctccaaaatgcctctgtccctaggatgatggttgaaag ggggatgggaatgaaatgcacaggatattaatagctctttacaaaaatattttaaaattt tatatttcccaggcatattaacttcaaatattttgtgctctcaagtggatgccaagccaa agcctcaaagcctgtaattagctaccttctttgcaaaccctgcattaatcatcttatttt gagatgtggggaaagttggagtgtatttttggggggccatgaataaaacaaagatagaaa tagtttgatttcagcaacttcttactcatgagtatgttaagggataggaggagtatccca tggctattaacagtttctactggggacaagcaccaacctatcaaagcacaaaatataata ttttgaggaaattggtcctggaagaaccatggcaccatctggttcttggaaaaataggca cgcgtttactttacacttgaaaaggaagcttttctgctctgaattgtactgtttcaagtt gataaacctgtttctgaagggcacatgttgctttctttattggatgcttatagtgacaca ttcctgtttgtgggggagctgcacagggtaggcaggggaggtgaagactcagaggagaga cagggaggaggggttcccaaatgtcttgatatgaaaaataggaaacagttcctttttgaa aacctgtaagttaaccagcagtagcaacaacgatgaactgtcatgcagaagactggctga ggtttgggttataaaacaatatttgccatcaagagggttttagtattccagtttgcagca ggagtggcttgtggccaggctcagccttgctagagaagctcagagggttttcttataagt cccaaactatttaaaaaacagactcaaacacagtgatatctcacttatccagccccttgc aaaacagatgttccacagaaacacatttctagatgatatttaggtttatgttataattta ataaagcttgcaaaataattttgttcatttgtttttcttatttcagtctgatccttataa caacccacggaatacagcatgggaggctttattaatctcactgacttgcccacacaacag aggtagcataattggtttttgccttgctccccatggctctcagacttctgaccctaggta tatctcagaaactttttcaatatattaaagacaggataatttgtgaaatcctactagtat tcactcctaggaaaaagaagactgaaaccagaattgcgttgaatataataagggttttaa gtatctttttaatattttaaatatttaaaatacctttaaatagtgccaagtactattagg tactgtcaaagaacaaaggaagatcctggacttaaggaatctctgggatgaggtcaatgt ggtggctgggatggtgctgggccagggggcagggagtctgggaggcaggccatttctaga agggccacgcaggaaggaaagacaaaaagcgggggctggatgtggggcagacatcagcct gagagggtgggcagagtataatggggtccttagcaagacaacgcctgagctgggcaaggc aaggtgagattatgtggagagccacccactaccaccatgttagcagtttcttcatgtatg ggatcatcctgtccagcccagtctcaaaGAGACCATTCCCAGTGCTTCPrimer
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