Human element [ hs2729 ]Position: chr7:134,619,121-134,624,806 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: CALD1(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence CCAAAGATGATAAATGTGGTGGtggtgtgttggcttctgattctgtgcttgcatctaaac tcgttcttttaattatctttcctctatccataggtctgcatgaggcaagaggcccataac tccttacctcactcatgggtttgacgttgaattaaaaaaatgataaaagctggggtggga aagagacatgaagaataatggtcagacatttcatcttaagaaggcaaaaacaggctgggt acagtggctcacacctgtaatcccagcactttgggaggctgaggtgggtggatcacaagg tcaggagatcgagaccatcctggccaacatggtgaaaccccatctctactaaaaatacaa aattagctgggtgtaccggcacatgtctgtaatcccagctactcaggaggctgaggcagg agaatcacttgaacctgggaggcagaggttgcaatgagctgagattgtgccactgcactc cactgggcaacagggagagactccatctcaacaacaaaaacaacaacaacaacaaaaagg caaaaacaaacccctggaaattaactggtaataaagagggggaaaggaactggttggaaa ctttccttaaaaaaaaaagtcaaaaccctgatttgccaacatgtagacctcagcttaatg tagacttgcagaaagccattaatttagggtagtattgatttatatttgactggtaattaa gagcatacatgctgcttctttattatctgggtgacacaaattcatgtggcctgaggaagg gtggaaatagtaggggggaaatgtttgtaagctttgagaaagattttttttctgtctgca agactgcattgaaagcactgtacctaatcaccaccacaccaccaccaccactcctaaaaa aagaaaaaaagagatgctttcacattcacctttccagagcaaattctctgtcactaagcc tgcattgcactgaaggtatctttaactcctgcttctgtacaagaggctcatgaacacagc taaaattgagaccatgagcaaagtgcaccaaaccacacacgaggtttgtaaacacacgag gagtgccacatcatgctagatttcagtgagcgcaatggcaggcacacgagaagatgttgg ggtgggaagaaaatatcccaccttgctgtttagaaccactgtgctgtgagcgctgagacg gcagaagagaagccatcccacgcagcacttgcaaggcgatccgagcaccctgtcactctt gtaaactagaaagggcttcttttacttgtgtgaccttaccattcttgaaaataaaaaggg agaagagaagggaactaaagtgcaagctaaaagagaaaagctccaagaagacaagcctac cttcaaaaaagaagaggtaaatatgattgaaaagtaatgatcgtaaagcaacagaaaaag cagtcagtgttccaagatatctcaggtctgatgatctcaaaattgtaacctcatatccag tgtatttcaagccatgactcacagtccactgaatagtgagatcagcttagagtgtctcaa aattaattaaatgtatcagtcagcattttttaaaaaatgaaatggatagaagccatcaga gggcattgtaggtagtaagtattgcattgtcaggtaaattgtctttttttctgtgggacg tcatcaaaaaggcttgaaaaacactgctggaatcaatttatcctgttttctattcttctg aatgctaattttttttccttgagccattctactttcatttcaatcatgaaatatttccaa ctggccttgattaatgctttaacttttcaaaggaaaaacacccacacacatctcagtaga aaatatggtgaactgaagatgatatttggttttcaaaagaaaagtttggccaaatgttct gcattgcatttctgaggcacacacaggagcgggtgccagggtatttgactgtaggtaagt gaacaaggagctatacagatagaatggcacggggtttgacagtaatcagaacaccacatc agaacacttgattgcacttcaactctcatgctgtgtttgccccaaataatcttaaaaatt gtgactatatcgaataagttcacaatacttattaggatgtggtgaaactgaattatttga agtaggaagacccgaagttcttcgcctatgagactggtgaagtgatttgtagccaacatg ctccaacccatctattaagaaaactatggcatctattaagaaaattcaaaatcttaaaca gagaaatccatatttagaaaacatggccagattaaatagggggtgggttattttcttaaa tacgttttgtcaatttcacgtgaaaaatgaaaacccctagttcatgttacatattacatt tctgttaagatgtatgttcctctgtttctaatagaagttgtggtggtttggaagtgaaaa ttgaatgtgagcacaggccatgtaaaggaaggagaaagtgtaaatgttaataacaggaaa actgaatttagctctcatgaggcagtcaaggaaatcattcattgaacatgttgggaaata cttttcatttggtgaccaaatacctacaaattcatttacaaaaaagaaaaatgaaagtta cctcagaactaaactcagaaatatgtcccacatgggttcttgtaatgtttcttggtgaat gtctttatagaaagaagtgggcatgaaatgatgactcagtaattaaacacatgggggaag ggaggctgtagaaaacactggattctatatttaaaatttcattcagttcactaatttgtt tttactgaccaaagctttctatacctatgtagagtgtctgagaactagaagggcccatca gttgccactcggatgatccttttgtctcttttcagataaggccccagttcaacagcgtcc tctgtgtcaatcagatactcccaggaatgagtgagccctgccgttttcccaagttgcccc tcagcacattccagccagttccaaaacttttggcttgtttttgacacaaggccagataag ctactcaaactggtttttttaaaaaaagaaaacaaccccaatctatattccttttttaat actgtgtacataacctgctgctcgaaagactaggttttcccctttccagctagttgtgtt gtttctttatgtagacagctttaaatcatgtttacatgattcagccattttaaacaacct cttcctatttttttttcctttttgtacaaaaaaaaaaaaaagaagaagaaagaaagcaac taactaaaacctttcctttcaaggatttatgcagcataggatagggcaaccacaagtaaa cacaaacatcacgtgagccttcttaaagaaaacatttccagaaatcactccaatgtctta aaaaacacacaaaataaaacctttctccactgcagtttaactgtggcaatgagttgcaga cgatcaccaacactgaaacttaatttagcttttttctctcctctccaatcataaaaagtc tctttttggttcttcatgcaggagctattttctttcttttctggcctctaacaggaaaac agagtttctagccgagctgctcctgaggtattaaaagtgatgttcgtgtcatgcggatca atcctgcccaaacattagtgtgcatgcaaatcacctggcagtcttattaaatgcagaatc tgattccatgaggtccatgttgtccgtggaccaaacttgcagtagtagcgaggagtctag aagacgtccatgttatagaaattgaacccaggaaaggatttggggcttatgaagctaaca aaagcacgtaaaacccagcctgagaaacagtagctacacccagctcttgttgctattctg gaaccaaataatgcaaaatatgctcgagacacatctcagttcttgcttgcttgactttct gagtgtctctctggcacaagggcctgtcatttgaattcccatcccccacctcatccccat gtattattccactggatatcccaaatatctagagtttcaaaacccaaatgatcccatttg gccagagccttttttatgagtatgctaatgttatctgtgtatgaagcacacaaacttttt caggatacccggctatctattaatccttcagcacggacgttctccatggtaacagtctga cctataagatctaatgcctttcccagggggctcagaatcccatgagtttgggttaaatct gccataacatctaaaaaaaaatgtaaaggctctacggaattactttatttattcatttcc cagaaagagaaaataattttcagttgaactcaaatcgtcattattacattgacaaggttt ctaaggttttccaaaaagaatataagcctgccatccactatagcctttgccaataaaggc agagcttgaaaaaaggggggattttttttctgttagacatgtatcatcttacaaatctat tttaaacacttttgggaccagtaaatcagatataatacatttggggagaatggaaatttt tctgactgcaacataggataaatgagttaaagggaatgtctccatgcgtagtgctccaca gaccgtgagctcctactcggaatttatttcatggttatccctaaaatgggtttccctcac atttggagccacagatgcttcttaactagaacataaactccagtccatggtttctggggc taagtctaacaacaataggccaaaatgagaatatattttgtttcctccttcctgtccagt gaatactgatatattcttgggcaaggacagccatcagggagccacagtgaagctgtcatt caggctatgagcttgctccttttttctgtagcctctcatgacccatgaggacccaagagg gaaatgacaggatgaaaaattcggaaggaatcatctggtccaaccgcattttttcacgta cttgggaccagcatcccagagcagtcaagtgatttgtccaagtaacacaactagtttgtt gtcagcactggagccaaaagtcaggtttcctgatttacagacaaagctcttgcagagaat ggcaaagtacagcccccattttcagtgtgtccgagggacatcctaggtatataccccatg gtgaaagattgaagtcttaactaattagggcagcatagaagtgaatccctagtaattaat cagataatacccaaagaagacaatcaaatgacttaaaacttatcaataaagagcaaaaaa ctgtgctctgctttgtaaaacatgcactttaggccaggtgcagtggctcacacctgtaat cccagcactttgggaggccgaggtgggcagatcacttgaggtcaggagtttgagaccagc ctggccaacaaggtgaaacccattctctactaaaaaatacaaaaattatctgggtgtggt gttgcatgcctgtaatcccagctactcaggatgctaaggcgggagaatcccttgaacccg ggaggtggaagctgcagtgagctgagatcgcaccactgcactccagcctgggcaacagag caagactctgtctcttaaaaaaaaaaatgcactttaggagtaaatcttcacagagtatct gaagaataggtgagaagaggtgagtGATCAGGTCAGGATAGAACCTPrimer
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