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Human element [ hs2728 ]


Position: chr6:85,708,190-85,709,032 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: TBX18 - NT5E

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
ATTTGAAGCCAGACATTCTCTggacttttcgattagtttaaccactaaattggctgaaaa
tatcctaaaccatatagtgaggttgtctgctgaaagtgaagggagaggggcataacttaa
aaaaaaagagagagagagagaaaatcagactgatagcataaacagaaaagggaagaatct
taacgaataatcacccagctaggccataaaacacagttcagtttggctgaactcaaccat
gctaatgtcaagcccaagtcaataatttggttttttaatgttctctgtggcaaccaaaga
aactgatttttttcatcacttccaattgcctttgtgaagtatagaccgcctttggctggc
tcatatgacacctaacatctggataaaaattgaaaaatgtcagccatgtgttttgtcaaa
cacaattacaaacccctgggtagttagaaaatatctaataattgtttcagatttcatctg
aatcatatacacacagcatttctgattcaaaactttcagagttaaggaacataatctctg
cacccaccattcctgttttacaagtcagcccccagcccaaatgtgatgtcaccttctcct
ccaacttaatatttcccctgtgtagtgttttctcaatgcaaatgcatcaaaatttcagct
ccatgagcaactattcatgttggaatcatatatcttatatttcaattttgttacataaaa
ggcagaatttacaatattcaagttagaaggtatatatggatttaaaagggtaaagcaaaa
ccttgttgctttttttaaccaggaatgaagctgatgttCAGGAAGGTTAAGTAACTTGTA
CAA
Primer
(+)ATTTGAAGCCAGACATTCTCT
(+)TTGTACAAGTTACTTAACCTTCCTG


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