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Human element [ hs2727 ]


Position: chr4:1,011,414-1,014,357 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: FGFRL1(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TGCACTGGAAAGTTGGAGgaggccgtttccagccagtgtcctggtcctagctcgtgtctt
ctcccaacagaggggtcatgacgctgcacgtgtgtccctgcctgggtgtctctccctgtg
ctgagggctgtgttctggggaagcaccccctccttgctccctgtaggagccaggccccat
ggggaggagccacctggccacaggagggaagccgggcagccccagccctgcatcctctgt
acctgccctggccggcccccacacccacaccgttctccctacgaatgtccactgagtatc
tgctgtatgccaggcctcgggacaccacgagcacgggagaggggcttgggtgtgggtttg
gaccacgtggccaggacgggcccttccagaagagatattggagcccccgaggagctacgg
ggtgaggggtgtcggagagggcacttcagatggggcaggtgttcggggagctgatatgag
ggcctgtgaagacggggaagggcaggccgcagggggttcctgcctccgtccctccagact
gcagcttcttcccctccatcccagggcctgcccacctccaccctccctgggccctgggaa
agaggagcctctgtctggggacagtgtgtgtgtgacaaagcgtgtaggcagagcggcctc
tgggccttgctgctgtgggaagcccctgctcctcctccttccctagttgtgttttttaca
gctgggcaggggtggggggggcggcgggaggatttggaggcatccgccttcttggcgcac
tgcctccgagtctagtcctccatacctgacagcagtgaggccctgtctcagactcagttt
actctcatgtctcgctcccatgtggcccttggtgtcctgcctgtgctgggggcctctcgg
ctgtggctaagtgtgtgtgcagggcaggggctgtgctggaggaggaggctggggggtgtc
ttggcagggtgcacggccccggaggtggcacagtgctgctgtcatgccccggcgggggtt
ccctgtgtttcttcctggctgcagtgcgaggcagccggtcttcccaccaggtgcctgtgc
cctcaggtcctgactcagtttccccataggctgcgtgggttggacgcccgtccaggtggc
gtggtgccctggccgctgtcctggtgtgatcattccccaggaatctggggggatgggctt
cctcctgggcaggccctcctgcggggggtcttgggccggcaacagcagggggtccgggga
gcccaggtgggggtgggataggcctggcgagggggagggaggctgtttgctttaggataa
gcctggctcctctggcccagctgcctctgcccgtgcctacagcgcttgcaggccggccct
gggggccacgcttccggccatccagcggtcatttgtctctgccccctttctcagtggtga
ccacaggcgggggtcccgcctccctgtctgtgcctgtcatcctgcacctcccgcatgcct
ttggggacccttacctcaccaagccttgcttgtatctgcctggggcagggtgggggctgg
gaaaccttcccgaagtgtagctggggctttcagcctctgaggtgggctccctccccagat
gtggggggctgggtcagtgtgctcccacccaggacagggccaggcactcgctctcttgct
tcttctggacagttgtgtgggtgaacacttgtgtgcacccatgaacatgcacgtgtgagg
cctgtgcccaggtagccagccaggaggcccaggcacccctgcaggctgtgggccacaggg
gctgggctggccaggggccttggccactcccccttggtcccgggaggccctgtgggtggt
gggatttcaggcagcgacaggtgctggtggggtcaacctggggtgcgtctgggacctggg
ccggctgggaaggggtcaggaggccccagtgctgtgggccctgcagccgcttcctccccg
ggacaaatttgccagagctgcccccagccctgggcagctcacagaaccacactgaggggg
tggtgggctggcagccccccacagccgccatctcatttcctctccggggagctgcaggga
gggtagggaaggaggggcttcagggctggggcccatggcctgggtgggagggtcctgagt
tgagggagagacgtgagtcagagaagagacccccacatgttttctcagagaggccaagga
ctgggatgggccgggtgagccagggctgagaagaatccctccctccagagcctcagccca
ccctttgtgtttctgggcacagcagggaggcgccgtgtctccactcccagccccagcggc
caatgatgcctgtgggctcagaacctagaggccctgggcaacgccccctgtgctatcagc
actgattgggaggcctccagagaggtgatagggattctctgtgagggtccctgtccccag
acttgggggtcagaatgggagctgctccctgagtgtctgaagacgccagtgggagacgtc
acagggccactgttaactgtgtattggaggaacacgcggccccaggccccatcgagatgg
cctggagcagctgctacctcttggtgttctgcacgcagcctggacgctcccagacagcag
tggctgcagtgcgggatggacactgtcacccatgggctggcgagagatgccctgggaact
gtggggtgcctgcggccgcctctccgacatggagagcagtgagtgggctgtggtcgccgc
tgcaggacttgggagcagcacgtggggagtggggcacgcagggccctcctttcccaggag
ctccatccaggtacaggccctgcggtgccccgagtttctgaggcctgcagggtctgtgtg
ttctaggttcatcttcctggccggcttggcactgcccactggggcTGGTCACACACACAC
AGAA
Primer
(+)TGCACTGGAAAGTTGGAG
(+)TTCTGTGTGTGTGTGACCA


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