Human element [ hs2725 ]Position: chr2:43,537,731-43,542,588 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: THADA(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence CTCAACATGGATCATAAACTTATGaactaaaactgtaaaacttccagaggaaaacatggg tgcaaatctttgtgactttggattaggcaattattttagacatgaaatcaaatgcacaag caataaaaaacagatacaatggacttcaaaactaaaaacttttgtccatcaaaggatatt atgaagacagtaaaaatccacagaaagggagaaaatatttgcaaactgtgtgtcttataa gggattaatatcttggacataaaaagaacccttacaacttgagaataaaaagagaaataa ctcggctgggcgcggtggctcacgcctgtaatcccagcactttgggaggctgaggcaggc agatcacctgaggtagcgagttcgacaccagcctggttaacatggtgaactcctgtctct actaaaaatacaaaattagccaggtgtggtggcacatgcctgtaatcccagctacttggg aggctgaggcaggagaatcgcttgaaaccgggaggcggaggttgcagtgaaccgagatcg tgccattgcactccagcttgggcaacaagagcgaaactccgtctcaaaaaaaaaaaagag acaactcacttaaaaatgcgcaaaggatctgaagagaccgttctttgaagatatataaat gaccaactagcactagaaaagatgctcaaactcattagtcattagggaaatgcaaatcaa aagcacaattagatcccagcactaggatggctattaataaaacagatggacagtaagagt tggtaaggatattgagaaactggaaacttcatacactggtggtaaaaatgtaagatggtt gcagactctttggaaacaatttagcaattcttcaaaaagttaaacttagagttggcatat ggcccagacattccacttctaggtaaatacccaagagaattgaaaacacaggtcccagca aaaacttgcccatgaatgctcatagcagcattattcataacaacccaaaagtgaaaacaa cccaaatgtccatcaaactgatgaatggataaacaaaatgtgatatctatatactggaat attattcagccataaaaagaacaaaatgaccaacatggatgaaccttcaaaacatgttaa gtgaaagaatccacacatgaaaggccacaaaggtatgattccatttacatgaaatatctt aaatagactttcaaaaactacttgcataaaaatttaactgacatgggcaactactttcag attatctgtgatgtgcagctggagctgggaaaggcaggaacagagaacactgaaaccaca gatgtggccggtcatggtggctcatgcctgtaatcccagcactttgggaggccgaggcag gagggctgcttaagcccagaagtttgagaccagcctaggcaacaaagggagacctctctc tacaaaataaaaaattaaaaacaaaaacagaaaaaccactaggttggcatagtggcatgc gcctgtggtcccagctactcaggaggctgaggcaggaggatcacttgggcccatgaggtt gaggctgcagcgagccatgactgtgccactgcactccagcctgggtgacaaagctgcaaa aaaaaaaaaaaaagaaatcacagatgttctgagaccctagttgttgactagaacggtatt tcactcctgggatgaaaatgtgggtagaaggtttcatgacacggttctgccacagacaca actctcacattatatgaggcctttaactttggtcacagaaatggacggtgaataaggagt caggaaagctcacccttttcactaaccagctgtgtaaccttgggtaagtcacttaaattg ctatgatctctaaactaaatttctaaagaattgtgggcctctgtactcagcgcaaggttt ggaattaacagagtaaacaccacgttaacctagcaatctaataacataccctatattatt ctgacttctgaaagacagctaggaaacatctgttattttagaagctcactgttccctggg aaagataattttggcacaagcagcagttcagatgacagttccagtgttttggggaaaacc aactaatttattgacaacaaagcctgttttttttttttttttttcctcctccttaaaatt tcatagctataggcccagaaatacaaagtccccagaagactctaaaaatatgttctcaat tcctttttatttcccacccagagctgagaaaacagcaccaactgtgtcatattaaacaca tctgcatccaacactgataaagtgcatgaccgaggaacagataagaattgttttcaattt gttaaaatggaaatagttaaatttaacctaattagactaaataatcaaagcaggtgtttg taagatgccagtgtttgttttcattctctgtaactgttcagctatatttcccaggaactt aggtaaaggaaattaatatgtcataaaaatcactgacctatttattcatgtcacatatgt attattcatgttttaggcagtacaaatacagggcattgttgaacatttctcacagatttt accatttaagcatcatcaacatctaagctggcctgcagaggggcttatgttacctaactt ccattatataaatacaagcagtaataaatgagagcctgtcttctaatcgtacactgatct acagagcgcactgacttggcatttagactatttagggtttctgactccacttgcacaagc tggtcacttttaagacatttaaaagaaaaataataacaataataaaagttatattccagt taaaacaaaatgtttattgcacttggcagagtgatgagatctgaactataaatatcaact aacaaagaaaccacaaaatgtagtcaccagcagcctggtattaggaagtgttttgtgcag tggccataggtccactttccaagggaaaaagggcaggtggctgcccgggagcagtcaagc gtgctctttctaataacacgctccagtcctgggtaggctctcgtattattccacacaccc tcaggatgggaaacagaaacacaagcaagaaaggccccataaaagcctgtgacgtggaag ctcctgaaaagggggtcccttctctgtggcctgggcattctgcagcagggacagaagcct gggaagttgagtccacgaagagggttctcggactcccttccctaagcgagtgactgatgt ttcctgactctggccctggttgtgccgtgaggccacagtgagggcagctggagagctcag gatctgccaccttagcacacatgggatgttaggcacttctcgctatgcctcagtttctgt ggtctcatgaaggaacattagtactttcttcattaatgcacataaaatgcttggcagtgc ttggcatagagatagatgctcaataaatgttggccactattattatagccccctacaaac aaggaggatgagattgaaaacaatgtttcctaaaaatgagggtataatgggcatcctatc catatttcaggggtaggaaaacagccttctgagatcatttatatgaagggtatggatgga caaatccttatcagcctaaaatattcctatctatgattgctatgtcatttctttaggatt aatcaatcctcttcatgagtcatatttcaatatattacacatacacataattataaaaga accaagttgcataaaatttataaaatggagactaggaaaggaaaaacccgctcataaaac ccaacaagagagcttaaaatgaaagtctgaaagtgcaggtaggtatttgtttaaatactt ggtaaaagaaaatgttcaattcagtagctgtgacagacacaattttaaggggtgtgggca taagagaaacagaaagtccatctgtcaaaggggtaaatatttagtcaagtcttagatagt atttctgtaaacagaaatttcaatggaaaatttccaacctgagagcaaataatttaagat gggtttaatataagaattaaagctctcgtctccatgtcgctgcccagcctgagacccagc cctctgagtccttgtggcagttcggctttttctttcttgttccctcaactacaactggac actataattttctttccattccagcaggaaactgatatatactaacgatgacagctggat tcagaagaggaaaaagaacacaggggacagagagtccttgtgggtttaaaactcaaaaca aaacccccagatttgaaaattttaaaacctagttaatggctgaggaaacctcaaatgatt gggaagcactatgataaaagtttgaattacataaaacacatcattatcttaagcttttct aataatcttctctgcctttctccttcactgtttttctccttgtttatgcccctagccctt caacgtggttctaagacatgtcagaggtaggaacagccttccaaggccatttacagaata ggactggagaggggatgagcagatgctcctccatccaaaaaagtttccctgaaattgtta ttacttcatttcctttggaataatcaatccctttcaagattcatatttaaatacatttaa tttaaacatatgtatatacacataatgaaacagaattatgtttcataaaattagtaaaat agaaaataagagaaggaatctcccctgtaatcccaccattctaaaataattccttttacc atagcgctggctttcattctagtctgttcctccattccccactccctacctcccaaaccc ctaatattgattatcaaaatggctgggcaaaagcaaagTGAATGCATCTCCAGTTACAPrimer
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