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Human element [ hs2624 ]


Position: chr13:100,448,047-100,449,351 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: CLYBL-AS2 - ZIC5

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AGGGTGAATTCTTGGAGCCTccaggggtagaagaatgaaactaaggttgaacaccaatag
gcattctgctgagcatttcatctaagccccctgccattttgcaggggaaggctgataacc
tggcccaactggtgcaggtctgcagggcatggggcagggtggaatgttgtcaagagtttc
tctttcctagagctgtggatggataggtacacaggggaaagcaaagtagagagggccagt
aagtggaaaaaaactgttccacatagtcagagcaaagggagcctgaagtaggtatgagag
cccaaatttgtggtcaagacataagaggattaggaagaacagacctaggaacttctactg
gtcagggccatcatggctccctgtgctggcagccatgattgggctgcttcactggagaca
tgctgcatgccatcagccttcaactttgagggccacaggtggggtgggcactaacacatc
tccttcctgggtttggctggaggacagtggcctccttgaattttggcccattcctaaact
gaaggcagttccccaccatagttccttccagcctctccccttcctaagagggccaggctg
tggagctgaccatggtgctgaagatgatctgagattgattccagagaacatttagggagt
acttcctgtgtgctagcactatttctaagtgctctgcatgggttaactcatttcatgttc
atagcaattctatgaaagaggtgctattattctccccattgacagatgacagataaggaa
agtggaatacagagaggttaacaaacttgcccaaggtcacacagctaataagcaaggaag
ccaggcagagctgcactttgatcaccacatttgctaatgaaggttattaacagagccaca
caaaacctcctggattttcacttgtccaatatcatcctagcagaagtgagccaaggttcc
ccatctcaagttgccaggcaggttgctgtggggtggcatcttggagagtcagacacagta
gtgtagactggttgggtacaagcaaaagtgggcagcccacctactccatctagtcagtca
ccacaccctaatgttatgacttacttcatacctgcttccctggtaggctcttggctttgt
gaagagggtgccatcgtatcccccattgcttaccagggtgcccaagacatagtcatagta
ggtattcagcaaatattttatgaataaatgggttacatgtgatcaatatatggtagctgt
tattaatttgctattatttcaaCATTGATAGAAGCACCATGTTGT
Primer
(+)AGGGTGAATTCTTGGAGCCT
(+)ACAACATGGTGCTTCTATCAATG


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