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Human element [ hs2581 ]


Position: chr10:105,842,616-105,845,323 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MIR936 - SFR1

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AAGGATTCCCCAACTTCCAGagggcacgaagtgatgacctacttagaaatcagatgccct
ccttcagggcctgggagctcctcattggaaggtactttgtggatataacctgctctaggc
aggtacaggtagatgcctctctctcaatctgaagccaccccaatgtctgtgtgttccaca
gccaatctgaggtgccaggtacgtgttgggagactgattcctttaaacgaaatataagag
gaaactttctataagtgagttatattaaatggagagtgatggcgtggggcacaagcctgg
actaggatcttcccatgggacctacagtatattcccaggacttcttttttacacatttta
agtgctgatagtttttaaaagtgtaacgttaggaaaaggaacgtttcctgcccaggtaca
tgcctgagcctgcctatcctagcaggattcaccagtgtgttcgtcttccaaaagtcctac
ttaatggaagcactgtcaaataacataatttttaacttctcagatatttgggccacttca
ctagaactaaggatgagcagatcattcaagctctcaattccaaaattatactaatttcca
tccatagttctaaagggttgctttgtggctattttatagctaatgtttttgtgaaggtgt
aaaaatgatcccatgtcacttttctaggcaagaagacacaattttaaatcatttttctac
ttttatgagttttttcctttcccctttatttaaatgatgtcacgtctacctaaaactatt
cagccgatgctgtctaacatacacacacacacaaggacaaaccactgttagtgacagtga
accatcacgttaactgtttaccatcacatttgggtttaaaccatcacattatcttattct
aatgacacatgaaacactttgttcttgatacaacaaagagaatgaagaagctaagtattt
ttctctttattttttgttttaggaacaaatgttcctatactctgctaatgaattgcaagt
atttttatagcttctattactccattctctatacactttaaattttaaaacaaaacaaca
acaaaattgccaaagtattagaaaaccatccactggctattgacctgataacacagaagg
gcccagcagtttgatgccaggaaagccaatgccctggaattgaatatcttggaggctttg
tctctgtaacaccccagagatgggtttgctgcttgctacatttggtatcctggattttca
tgactgggagcaggtgggatagggcaccagctttgtgattatgctggtttgtttacagtt
gtgtcctgccactgttaataatggcaaatgaaacaaacaatgtaaacacagacttctttt
tgtttttcataatctgagaaggtggttagttcttaggtcaggtcagtttcaccaatctaa
ggagtttttttcctatttttttttttttttgagacggagtcttgctgtgttgcccaggtg
ggagtgcagtggcacaaccttggcccactgcaacctctgcctcctgggttcaagcaattc
tcctgcctcagcctcacaagtagctggggttacaggcatgcaccgccacacccagctaat
ttttgtatttttgtatttttagtagagatggggttagtataatatgagtgtgcaccacta
cgcacaccactatgcttggctaattttgtattttttgtagagtcagggttttgccatgtt
acccaggctggtaagtttctatgcaagcttgaagcagagctgttcactttaattgttcct
gtgtaaactctgatcagactcatgtgggtgttttgcagggcctaagagtaaagcaggaca
cctctgaatctctggagtgagtctgtgtggggatagacatctgtcttaggttagttctga
cagatctgtgccttagctctaagagagaacatatggaggtaagaagaaactattctgctt
ttcaaataaataccttttaaaaatcttgctacgttttacaagtccactattagactactg
attaactacttctcaaaagaaggaatgtgccagaggttttgcctccacttcaaaaagtaa
agtgataatttgtcagttaccagttaacgtcatccacttaggtatctaaactggtaattc
tcatctcaaagtgaatttttttggcaggaggaaacccaagttgactcgtaagagtatata
tacgggaaacaattttcttcctttttttattcctctaagtttgatcctgcaaaatttctg
aattaatcagatcatttcaaagatcctacttttctttagattagaggtaattttacagca
tggtaaacagcaatgtttaccatgtatggcttaatcagagaggcccagtcagcacaggga
ctgtttctagaaggcccccttacctatactttaacttcacccctgagttgttctcttcgt
ccagtggagagagagacagagggaagaggagagcaagatagcgagagaggaaactgagat
tgggagagaaggaattataagcccgtatcatattctaaataaaacaacacaagtcatttt
acaatgctgaaaacaatgcagaaaggtcttgcctctaaagcccgctatattttaaccagc
cttcagccttctccttttccccacttatttcctttgaaagacctacCCTAAGCAGAATGC
ATACACCA
Primer
(+)AAGGATTCCCCAACTTCCAG
(+)TGGTGTATGCATTCTGCTTAGG


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