Human element [ hs2575 ]Position: chr2:221,966,861-221,971,455 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: MIR4268 - EPHA4 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GGCAAATGCAAATCACATTGagaaaaaaattcaggtatctacattgcaagtataatcaaa taggaaaagttccagctctcaattttgctgctgtaactatgatcccatagtttgatacat gatccataatctactcaacactgagtaaatatactatgaattttattatttatgccagta ggcattaaaattccatcttttttttaccctgccagcattgattacaacacaaggacacac acatacatacacacactctactatggagcagctttagagctttaagaataatttgggctg ccttggaataccagaagccctagaaaaccagcaactacactggaagatcttgaataacac gggtggttgtctagaaaaagctacaggctctgaagtcaggaataaatctaggttagtatc ttgactctacccttcagcaatgtttccccttaagcatatctgatgacatctgtaagctct agtctaaaagagtgccactaatacctaattcacaaatctgcccatgagaatggaatgatc ccatatataaactgcttaacagtgactggcacaaagcaagctcctcaaaatacttagttt cctgctattttcctctccttgacaagtgcttcttctggtgaatctccagggggttaagtg agcatggatacactcctgagaatttcaaaatccttgggatttttttttttcctcctgaca gtcagtactgttggctggtttggaacctcctagtgaattttctttttctcaaatcttgga gtatttttggagtttatagatcagtgtcctcccaaaatggctcagcctgacctaccccca gcctgtctccaacactcagactgtgttttcttgaaacgtgcgatgctttcctgttccaac tacatgttcctattcaatcatccattcattcaacaagtatttaccatatgccaatcctgt actgggatacaaggtgactaagacaggcttggttccaccttcaaggagatcatgttcttg cagaggacacagatgaaaagtaagagggcacataaatgctttaattataactgataccgt ggcaagtgctgcgatgggggctctagcagagaacagaagtttaagtatcggggcagtggt taccacctgacatagggcatcaaggaaccctccttgaggagggatgagttaggcagaggt gagctgcatagggagtgagagacctcaggcaggaggaacccagagaggttgttcaggagg acctctgaaataaaaactattggcaacagggcactttgtctccctcccctccccttccct tccctttctttcctttctttctttcactttccttttgaggcagagtttcactggcagtca ggctagagtacagtggtgtgatctcagctcactgcaacttccacctcccaggttcaagtc attctcctgtctcagcctcccaagcagctgggattacaggcacccaccaccatgcccggc taatttttgtatttttaatagagatggtggggggggggtggggttcaccatgttggccag gctggtctggaacgcctgaccatattcttgtttctagattcaactccattcttacaatcc tgaactcctagctgaggatttaattgcatatataatcttagatctctgtgacttccacct tatttatttatttatctattcattttaaattttctgtctatatgtcaagaggtctactta ataaactagattcctttccttttaactgaaaaaaattagagaggagtgctgcctggagcc ctgcctccttggcgttgtgctctacttttttatctacacaggatttgtgtcaatttgttg gtcttacaaatgctcctattgtggggaggaaaccatgggctgaacacaagggctgcctga cttcccagtttcactccaggaggcccacgtcacaccagcttcttcttgatgctgtagaca cagtcacaaggtgtccacatgtgtcttcctcacacccatccgctgggaaacaggactctc cttcctgctctgcagggtggaaaataggaccccaggcagtcgctcccttggagatgggtc ccacactccatgaagctcagcatgggcataagagctgtctggctgcggtggctgcctgtt ttcagcaggggtcttcagccagcattttctccaggagggtagcactgagctttcttccag ctgatggctctggaaggcaccacaagttctttttggcgcattttccactttcctcctctc ctcccactcccctctctctctcctcaccccacacagatcctgtgctgattgactgccatc gccaggcttctgtaccaggcaatcaatcaaaagagaccttcccacgcggaacagccgccg cccctccaccagactgctgcctctgacatttcctgcgtgcctggactgagcctgtttgga aggctccagccccctcctatcagtctgggcactcagggctctggcagcagctgccgctct gaatgttgacaccctgccacgtcaacactggccgggccgatgttccttcacactcatctg ctgagcccaaacttcccctgctggcgggctctccctgtttgaatgggctcccagtcagaa agggctccctgaagccttagctccttgaagctcgccccttgcagccttccggctagagga tgtggctgttggatcagctcagtgatccttggttcacaaaaaaagaaattcagttcaatg tggcctttcccaaaccccctcaaacagcgttctgtgcagattgagcctgctggccttggc attgtccttccgacttgatttttcagactatctttcctcttgtctgtgagaataccatgg ggctcaaggacctgaaggaattggtgcatgtatttcatttgaactttgctgaagcttttt tattttaagccaagtgcttcctctgctgtctagaaaaaaaaaaaaaaaaaaagaaagaaa agaatccttctcttgccgtcttttttgcccacacagatagccgcttctcatttttctggt tccaactttcaggtagttgtatactaattgcaactaattcttgaatgtagactaattagg aaatacagaaaactacaataaggtaaattttaaaaacctccttaggccgggcgcggtggc tcatgcctgtaatcccagcatgttgggaggccgagaaggatcacttggggtcaggagttc gagaacagctggccaatatggtgaaaccccgtctctactaaaaatacaaaaattacctgg gcgtggtggcgggcgcctatgatcccggccactcaggaggctgaggcaggagaattgctt gaatccgggaggcggaggttgcagtgagccgagatcacacccctgcactccagcctggat gacagagacagactccatctcaaaaaatacataaataaattaaaaaaaaaaagaaaaatc cttcctatctaaggagcaagagataataatgattaacatgacaggatatatccttacagt ttagttctctgcattatacatgcaaagaataaacacacatggatgacttttctatgtaca ataaaagcaccatcacttgacacatctcagaaccatttctgatttaatgttttatgaatc ttaaaggcaggattactctttgacccaccaatttccccctagaatggaaaagtacatcaa agtgtatgtactaggttgttcatcaccaccattatcatattagctccaaattagggacaa tttaaattcccagcaataagagattggttaaatagattgtgggataactgtgcaatgtaa tactaggagaatataaaaaatggtgacatacatatatacataaatatatatacacaaaca tgggcatggaacactactaataaaaagatcataaattagtatgcataatactttacatgt atgcatctatctcactttggaagatgatatccaaaatattaataatatttatctctggcc gggtgttttgtgagtaatatatgactttagattgtctgattatgttagaattcttgtaat gaactcttatgcttgcgttaaaaaagttaataggagaaaatatagtagagatgatttttc catatcattgtttttctcaaaaatgactgttaatggttcgctaccaccatgtgtttattc atttccctatctcaactgccttattgtatactgaggatgggagtgggagggaatgaggct tagagacaagaagtacctgactaattaggaacaagcctgggctagaatccaagcctctgt ttcaaaggtctaaatcctatgctgctttccagttgttatagatctaggagtcactgatat atcctacaagtatgctgtgtgtatgtgtgcgtgtgtgtatgtaacacatatacacacaat attttcatattatttttaaagggagttgaagttggcgcataaaatggttatacttttcca gttccctattggcttccctttgaacttggaaaccatttcctacttgaacctcatctcaat tttctctacaccagtTGTTCTCCAACTGAGGTCCCPrimer
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