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Human element [ hs255 ]


Position: chr3:114,611,706-114,612,491 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MIR4796 - GAP43

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
tgatCATTCCATCACCAGGCATAAgagaaaagatgggggaatgcgatgttttattcactt
taaaattaaaaattggttgtagaaagcacaaaggcctctttgaatttaaatctgaaagtc
actgaggccttatggataccttgaaacagcccaattttgtgagaacatttcccaaatcat
tgggtacagcacatttacagaacatgctcttgggatccggacttcgctccaaaccttgtt
tgtacaaaaaggggccaagcagctgggaaattctggagtggagtgtgtcctcattagttc
ccatctgccaccagaactcagctcatggcaagtttggctaagtcctggtgtactcaggtc
caaatgtgggtcagttttaattacactatacagaattcagtgtgcagttttaaaatcctt
attatacagcacagaggaatgggcagctctccagctaatcaattttttaaaagtacatat
ataaaaattttaaaccaggctagacagacagtgggtggaaaaaaatctttaaatgagtac
aagggttacttaagagaattattagtgaaattgggtcatgaacaagttctgctttaaagc
ttcaaagtgagaaacaaactgttttcattggtaagttggaaaacttatgtaaggtacaca
atgtgccttgctctttagtgtgaataacaataagcctgtggccagggtacacaacacacc
tacaagtgaactggctttcatgtaagcagaaatgttcccaattttgTGACTCTTCACTCC
ATCCCC
Primer
(+)CACCCATTCCATCACCAGGCATAA
(+)GGGGATGGAGTGAAGAGTCA


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