Human element [ hs2504 ]Position: chr4:120,655,056-120,657,889 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: PDE5A - LOC101927007 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence AGCGTGATTACCCAACATCCcaatgaggtacagatactactttgtagaggaaagggtgat ggagaagtgcagatgattttagcggggtagtaaatgatttttaggggaattcaatggact tgaagagcatacaatgaactgggacaaagtctactgggcccacagagcgggcagtggttt gtgacaaaatcgtccaggtttgttgacagacttcagtcttattttctgtgatgagctcag ttaataaaatctccaggaagggaccagaggtaattattttttatttgtcaggtccagact ttaggcaaataagaaacttcagagaacagatttatcctgtgctttgggagacccagaaga ttgagagataggaggagtggggaagagtaggtcagagagaccttgagcctcttcttcagt tcagcatgtcagagtgccatatttcggggtgttggtttcttttttctttctttttttttt gagacagagtctcagtcgggagtgcagtggcgcgatctcggctcactgcaacctctgcct cctgggttcaagcggttctcctgtctcagcctcccgagtagctgggattacaaacacaca cggccacgcccggctaatttttgtatttttagtagagacggggtttccccatgttggcca ggatgatctcggactcctgatctcaggtgatccatccgccttgacctcccaaagtgctgg gatgacaggcgtgaggcacggcgcctggtcctgggatattggtttctaaaccccaacacc atttataacttttcagagttaaagtcaatagagtagaattttagcaaattgaagaaagat aatagggtcataatgtctacaatcttacaatgatgcttgattcatatttttattaaagag ccaatttagagaaaaattgtaaataatggtgatgatgtgaatcagagaagaggcaagagt tcagcaatatggaggcctttcactgcctatcctagaaaacgctgttcttatgtgttacgt ggagtatagataccagtgcttggggggctggagtctcattctgatgccataaatttgatt ctgagcttagattatttgtttaaagcagttatctgaagtgagacaacacttacaaataga taataggactgctgtttaaagtttctgaaaaaccacagagcaagacaatgtattgaaaag aataaacagtggggatttggaatgatctttctgaatcaataacaagtgacagaaaagaaa agaattactgattttctagctatttggaatgacacaacactttctgggattcacatttca tatcagagttgtttaaccagcaaaatgctgaagtgattaaatatgaaaccacacagctga ggctggagaaaactgtttgttcagaaatgcttctggaatgagactgcagctttagttatt tttgtttggttggtcagtgatgaaatgtgctatactcagcctgctaaagttatccacaga ggaaggtttactttggtggttttaaggtatctcctttcagaattttgatgtggcagcaca tcccttaacagctcatttcctgagggatactaaacaagaggcaaacctaggaaaaaagac aagtttaagatctctcacctcactgtgaactctttccatgtttaaacatagaagacactc tcataatctggcttcaactgaacactgccacaaaggccctaactatttattacgcagtga cccagggcctatttttcaacggaaaaataagagaagatatctttactagctttgtgcctt gcccaaacatgaaactgaagccaaaatgagtgaaaagactttgttttacctataacaatt tagttaagagaaaatttttataggtttatgtgcagagtcaacatcttttggttatccact ggaaatttataactatactaatttatatcactttattaaatatccataaaaatcaggctg ttagttattgaaaaataaggagctcacagtttgaaagatgataggcatgtggtattttta attatatctaaacttataagtcaggggaaatggtagatacagtttctagtgtaaagctta ggttgggattgaaaaggtagcatgattggtcagggcacaccttggggtttaacattttgt ggtgtctgttgggtgccttggacactggagatcttggaactgtatcctggaattgcatat ggaatacagtcatcacatatgcttatagagttagcacttcatggcatgccatggatatgc tattgtcatctgatgttttcgtcttacttcaatggtataatgcaaaatggttaataatgt aagtttttttcaaatataaaattacttataagacacacatattcttaagaagccatatta ctaaaccaagcaagctaaatcctcaaaacaggagacgaacagcagaaagggattgcagct acatggagagactcaccaatgtgtctgaataaagtgaatatatatttttgaaagacatca tttccaacggtaaaatgaatggaattatttcctgctaagactaatctgctataatttgat tggtccatattctttaaatgtaatagcaccagttcctgaaattcttaagttattaaagat acaatattttaaaggttcttttgatcacagttaaattacagatgatttttatttttcttg gcattttataaaaaagttaaaaaatagctcttaccagtggtgtgctggtaaacgttcact acccagctttcagggcagggttggggatgtcctgattcaaagtatttgatgatcAACCAG TATGCAATGCTCCCPrimer
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