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Human element [ hs2491 ]


Position: chrX:153,593,214-153,596,578 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: FLNA(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
agCCTCCACCACAAAGTCTGCTgacttgccaacgacgccgccctccagcccagggcccca
ggcccgtaccttctgattgccacactcggtgcccaccttcacttcgaagggactgcaaat
gcgagagccacacagggaacaccgaggatcaccacatgagccagcgtgggccccactgtg
gcggccaggcaggaagagcccatgtggcccctcatcatcaggtggggaggcagaaggaag
agaagaggcagagtgtgcagagctgggagagggatgcctgggggcctcacctgcgcccga
tgttctgaccaccccacgtgatggtgacgatataggttccagggaccatggggtaatact
cgaagccatacacgccatcccccaggtccttctgcttcacgcgctcctctccctctgcca
agacaaggagggcctcaggcctgcccagcagtgaacccgggggctgccgccacccatcct
ggcctggctccaggccaacttactggggcccttcacggtgaccttcagctccccactgcc
agcgccctttgtgtacaccttgaagtcagctgtctccttcacccgcacacccttgggctg
gaggccccggccaaccgcccggcaggcactcgggttacaggctgcaggcagaggggccag
ctgagcaccagcagctcggctgggcgacccctcccttgcctcccacagggccgggctgtc
aggattggtgggtccctcagagcttggctggagggggacatgcaagacagaactggaagg
gactgtgaccccaggaactgtggcctgggccaggacatggcaggcctcctcccacctgct
ccacaccagcccagagccccccagcctcccccttccaagaagaagacagatccaagtacc
gttgaccctgtgggcagagcagagagcagcaggtttctagacagctggagcgagctcttc
cgaaggtgaaagcgtgaggagagagatggagaggggcgaggagaggagaggaggaagggc
cccagcaggggaggaaaaacagcatgtgcccagacagtagaagctcaaagagtaggggcc
ccggggcgggctgcagcgggactggcccagggggtccccctcctgtgggaggcccagact
gcagtgccacagcagagggcagtcagggccgggcctaccttggccaacagtgacagtgta
ggggctgcgagggatgggcacgccggcaaacgtgacgtgcacggtgtggacgccctccat
ggtgggctggtagctgcagcggtatgtgctgtcgccccgggcctccagctgaggctctac
cgtgcccttctgtcccatggggtcctggatcacaacctcgacctcgcccgtgccagctcc
tgccacgaggcacctgctcagctcccaggccccagtgcggctctccccacagaccagctg
ggccttggcagcctcccctcacctgccgtaaagatctcaaagtaggtggtcttgttggcg
atgttgccactgggctccaggccgggaccttgggctgtcactttgctggcgtcaccctgt
gacttatccacgtacacctcgaaggggctcttggcgatgtgctggccagcaaagagcaca
gtaacctgtccccagaagggtgggccgtgagggtagggctgggggcctccagccactgcc
tgaggtcacaagcctcccccctggccaagggctcaccttatgagtccccgtcacctcggg
gacgtaccagacggagaaggtgcggttcttgtcgttattggcggtcacttttgcctgcag
tgggaaggagcctgtgagcctttgctaagagcagccccactgaaagggagcgctgcgggg
cctctgctgccagcagctggccctacctcctcctggtgtccggccgggtcctccacgtac
accagcacctctccctggccagcacttctggtctccacagtgaactctgcccgcttcttc
accatgttgcctgtgggctcgatgcctggcaggggaaggcgagccaaccacgggccagct
gttaaggccacagcctcacccctccacccttcagccctccctgctggcccctaagtcagg
cacattccaaactgggcaagttcattcagtgtgagctgtggcacagagcctggcctcggg
acccagctgtgctctcgctgctgggacaggcccagcctgttttctggaacattcattctc
accgcactgggcccttgttcctgtgtgagccttgactgggttttaggtccctgttactga
gacctggttcatctccatttataataaagaccgctggatgctacctccccgaggacccca
cgcagcaggcttctgtgactggaggaacctattagcattctcttgtcgttttcactagtc
ccaaaaacccactcttgtctgactcttgggtggcttgatcacctagccgccatgtaaccc
aagacccctggggaccgccacgtttagatgggacactgtcttatggggaagacgttggca
cacgggtgcacccctggtggggctccctcacctggcccgtaggcacgggctttcttcggg
ttcagtttgggccgcaagggagcccctggcttcagcttggccttggggaactgggacagg
taggtcatgacagagtgctcgtccacgttggggtccacaatctcctcgggggtgatcacc
tgtcacaggcagaaaacaggagccatcgggcctccgagtctctcccaactgccgatccgg
tcccctacagctgtagccaggccgggcgggtgtacctgggggatgcccagccagtcatcc
gcctgctgcatggcctctcgcgcattggtaacgggcttgctggcgtcccaagagtcccag
tcaggacacaggcctgtggcgcaagggaggctgtgagtctgggggccgcagaaccccctc
aagggccacccatgggtgaccccagcccagtctctcctgcctctgcgccccctcacccgg
ggcacagctgtccaccagggcgcccagggcccggccgctctgccagtcccggctgaagtt
ggtgatgggcagctgcggcagcttgttctggatccagcccaggagcctctgcttgggggt
ctgcttcttggcctcctcatcctcctcctcgtcccacatgggcatggagatggagtagtg
caggatcagggtccagatgaggcccaggatcagcttcaggttcccgtccacgatggcctt
gctgtctgtgagtagaagagtggccacgctgggcacacggctgtgcgggaggggccgatc
ccaggctttggggtcagggtctggcagcacggggtagcaggggccAAGGAGGAGGTAGAC
ACCCC
Primer
(+)CACCTCCACCACAAAGTCTGCT
(+)GGGGTGTCTACCTCCTCCTT


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