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Human element [ hs2382 ]


Position: chr3:186,123,841-186,125,332 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: DGKG - LOC253573

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AACTGCCCGTGTATCCCAAGaaatattgccctctttagtcaaatattcagcatttctgag
tactctatctgctacccctcaaattgatgagcaagatctgattctcagaaatctcatcta
actctgttggcttctaaactaaatattgggaagcagcacttcatcattcttgatggctct
ttctgggagtaagtgggaccagcagatatcgggtctgtccatggtccattttgtcaggac
cctgtgtcattcctgactgccccccaacaccaaatctcacccctgccagtgctgggcctc
cctgacctgaactcagctctcctcagtccggttcttggcctggatttcaattccattgat
ttacgtttgtttttccccccaattttcatggtgacacatctcaaagccaaagttgcctct
tctggttgaatgaaaaaatgtgcatgactttctgggttcgtatgtgggaccaaggatccc
ggatcaggcgacatttcacatgattttagttctgaggttaaccaggattcttaggatagc
gaacaatgaacgatgctttctaacaacatgtgacctgggtacaaactcattaatttacta
aaagttgctctttctgatgtgtctgaagcctgtagaggcaggacagtcgttccgactgca
aagtgctttgcaaagtatgaaagatactgagttgccggaggcctgcaagcaggaagccag
aactgacggcaggctgtcactaactgctctccaaaaaaacagtgaggaggaccaggagcc
tttgccatacgccaggggattactctctggaaaaaacacaaggttggctgcccaccaaga
cacaaggttcccaaatagagcctgagtccttgagggagaggagatctagctaaaaatagc
tatgacgtcccaaagttgattttcttctccccctcgccttttcattttttcagtgagttc
acctaaaatagcctggcaaaagattttttgccctttaagtggaaaaagaaaacagctaga
gaggtcaagagctgaagttaaggttctaagaagcctgaagtttccgcccaaaagacaagg
gggtgatggccctgtcccgggtgaaggtcgcctccctgaagagaagggccgaggcctgag
gcttttcagggaatgacatgagctcctgtggcccagtggacacatacagggggtgaccag
agcctggacaggcatccaggtcactgaagggcaaattggccttcagcttgagggaggatg
ccaggctctgaggtccagccccaaggctggcatttcaggaaacctaaggataatttgtgt
ttgggaggcagcatcccaggccgtcttactcagccgggaaccccagtgggcgggtcagag
gcaagatgtttcattatcacctgcagaaagagctccaacatgaggaagtagggtttacct
agagatagggccaggtaagggagatgctccccATCCCAGGGGCAGTCAACTA
Primer
(+)AACTGCCCGTGTATCCCAAG
(+)TAGTTGACTGCCCCTGGGAT


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