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Human element [ hs235 ]


Position: chr11:15,623,970-15,625,123 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: INSC - SOX6

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
agggGCTTCTTCAAATCCCACCAGgtgaccttaatgtcccaaaatattcttgacactgaa
gcactcacttgcaaagaaaagctggaccccaacccctgtattggacagaccctacaggaa
gtttccttaatagcctaaggagcagagacagaaaatgaaaaatcactaaggtagtggtag
gcttacatcccagggagggagtgccatggcgcacacctcctgcatgggctagggttcgca
tttgtgtttaataacaactttgatctctctgccagcagcagcggtcattgagaaatcctg
acataaagaaggtatctgggagataaagttctgcaagcgatgattactagcacttaagtt
ctctttttgttgaaaggaagaatcccagattgaacagtgcattgaatgatatgacaagca
agtacttgatccctgtccagctggaggggggattgtacccatcagcagctttttagctaa
ggacgtctttatatttcccttctttgtctgggcctctctagttcctccttttacttcatg
tcagcactatatagtaggtatgccaagtggttttgaaatttaaattacagtctctttttc
agatgttattggaaagaacatgtaatttataaccacgtgttaggagtacaggatgttcat
ggtccactgccaatttttcttttgtaaagttaataaaactttcataaaacacttatacac
ttgtatatttcatggtaaagattttttttatctcataggctaatgtaaagatttattaaa
accatttttttgttaagaggagtgtcaatagtttgcagctgagtgtaattttgaatccgt
ggaagatgacggtctgatggaaaatgacagtaacagctttgttgatttagcactaaaaat
aaaggagcctttcaaatgagagaatgctctaatatccagagtgggctaggacgcactcag
gccaatggaggcaagctggaggagccggccaccagcactccagcccctggcaagagcaga
gcaggattggatggagcccaggtgggagggtgagcagagccccaggggttagtgggaagg
aaggctcttcttgcaggggagggagggaggggccaggagtagtgtctgagtttaCTTCTT
GTGGCAGAAAACCC
Primer
(+)CACCGCTTCTTCAAATCCCACCAG
(+)GGGTTTTCTGCCACAAGAAG


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