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Human element [ hs233 ]


Position: chr12:24,291,012-24,292,724 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: SOX5(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
tAttTTGGAGGCCACAAATAGGTCgagctctgctttgtatttcattcttaaacataaact
tttagccaaaatttgccacttagaacaatgtcagaggaagtttcagtgcaccaactcaga
gaacggaggcgtatccacttgatcatcagctgttcattcctgtgttctttagcagaagta
cgaaagcaaaaaaggaagtgtgcttgcttttgagaactgattttttctcctgttcatctg
cgaatattctcttacatcgcagtttgcatttattttacaagggctatattgatcttatac
acagagatgacaccactcccctccaggctggcctgtcaatcagtataccatctcttcaca
gacaacatgctccagatttctttgctaacatagccagtggtgttttggtttagttgtgca
ttaagctattgctactttttcatgaacacttaaacatcagatctagagaacaggacagtg
ggcatgtattacagaagcatttgtgtagaggaggttggtgggggatgggtatggggaaaa
tgtttgcaagtcaggtaagaaaatacacgtttttcattttaagcatatttattcttaaaa
attcaggtctaaaatgctacttttacaaaatgaccttcagttctgagtgtacccaggcga
aagagcagataataataggtacacttcagagacataaaaatttctctcatcaatttcaac
tatgcacatctacaggatgatcctagagatattccccccattttaaaaattgctgaagga
tgttcaggctattgcaactgtgtgtatcccaggcaacataatagattatacctcgactcc
ttaaagagcaccctgataatatgcaaggctaaaatgtcagaagctattagaaactctcag
atgcacaatatatctccatccctgagttttccccttacactgcgaaacaccagacaacag
gcatgcaaactgctcagctgcaactgttccaagtcgaataattacaacttgtcagaaaga
attaattttcccatttctcataaagatgtgtaaaaattaccatgttattatacactgaca
ctctctggaggcagacacaaaatttaattttgaattctttccgcatattaaattaaatcc
ataaataagagtttaggcattcactcggaagcatatgttcattgggagcgaaagccaaca
cgagcccggctgtaatggctgttgtaatgttttaatagctcgcttcttctaagcaccaat
aaatttacatgatcataacagtcgtgggcatacaagaagcccattactgtctgggcacag
gcagcagcgttaacaggatggaaaaatggaaaaaggaaaaaaactcttaaataaaattag
gcaactgcacttgtcagctgctccatgattgaatttttaatattgtggtcaattggtcaa
aacaaatgatgggataaaatattttaatgtatttagaacctgtttctgtggttagcccac
aaaaatagttttttgtttgttggttttgcaaagaacggaccgcttcaatcttgctgtttt
gaacaattttatttttaaagacacacactgcaatttttaatctcttagaggacatttgct
ctccatattcccatatttaaaaattggagcattttaaattcggcttaacgggtgattctt
tttagccaatggaGTCTCGCCTGAAGGAAACTG
Primer
(+)CACCTTGGAGGCCACAAATAGGTC
(+)CAGTTTCCTTCAGGCGAGAC


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