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Human element [ hs2280 ]


Position: chr18:32,695,989-32,699,504 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MAPRE2(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TGACAAGACCTGTTTTCAAGGAaattaaatcctagtgggaaaatctgacaataaataggc
aatagtaatagaggcgagttccttcagcatatataggaagaacacctgagctagtcttaa
acatcttctataaaggtttctgaaaaaagtaaagttcaagcaaaacttaatgtataagta
gatgttagccaaacggaggtgtattgaagagcattccaggcagaaagacaaggatgctga
agtcgaaagaatgtgctccattcagtaaagtgcatgttgttcagtgtggatgttgagata
tgagactgcagagggaagcggggctcttcttattgctacaaaaataaaatcacaaactta
gcagcttaaaacaacacttatgcattatttcacagattgtaaagtccaggcacaacttag
ctgggttatctgttttaggatctcaccagactataatccaggtatccactggggtggtgg
tctcatgaaaggctcaactggggaaagatttgtttccaagccccctcaagttgttgactt
gaggctgcaggaccaaggcttcagtttcttgctgaaggccaccctcagttcctagaggcc
acttgcacctccatgccttatgggcttttccaacaaagatgcttacttcgtggcaactgc
ttcttcagggcagtaggggaagtctctctggtgtgtttgtcagcaagacagtcatgtagt
aacataatggtaagagtgactcccattacctttgccatgttctgtgggttagagtcaggt
cacgggtcttacatacactcaaggggaggctatcatacaaaagcatgaacaccaggaggt
ggagatcattagggtaaggggattactgtaggatttgtttgtcacaccgaggttacactt
tatagtagggagtcatggaaaagtttaagcagaagcatgctttgtattatagaaaacaca
ttagagagggcacagttagaatctcgtaaaccagctgagaggctttgcagcaaaccacat
gggagaagacagtggccttaatgaaggtagtagccatgcgggtgatggagaccgtaagat
gatacagtcagcaaaattggatgattaatgggttatgggaagtaaagcagaggaggaaat
tggggataaatgttacacacatttctgacttactagtcggttgatagaagcagagatccc
tggaccaggagcctggtgagcatggggctcaccgcggagttctcccttccctgagctcca
gcctgccctgtggcctcagaccgtgtctctcttagccggagagtctggataagatggtgt
taatggagcacaacaggctggccaccatcggaaaaacaaccgcaaacactggtcttgcta
gttattggtcaacaggggttctgactggagtctaaaggacaagacattatacacttagta
cttacgttcccccaaaatgaccatcctagtgtataagctttttggttaaatgactgctgc
gtaccaggagtgcagtagcacccttgatgtagttgttgtggtgacacctgtgtgtcgtct
gcccagtgggctggctcagtgtgctgggctctgtcaccatcttcccctgttactgcagta
aacaggcgaaggggtactctaaattattgttgggatgtggtgtgtgtggttttcactggg
gtgagtgagcctcaccatttctgttgctctgagaatgatcaaaaaataatattttaaatg
aagatagtgattggatgtgatacttaatgtgttttaaaggacacttaatattttaccaag
atgcgaggggtgaaaagtcagtagtttattgaaagaaatagcatgttgaaataactattt
aaaagagaatttttaatacttgagccattttaagatgcagaacgctttcctgcatttttc
cccctgtgaaaaattctctgaaaccagagtgactcaagcagagcctggaaaatactggag
aatcacatgatggctaaaaataagcacatcatctctagcagggacagtaagcagcagggc
agaagtaactcagtatgtgaaggctcacactcactggacatgctcaggagagtcaggccc
tctgtccttggcaggggcaggctttgagaccacaagtcagcgaagagaggtcctcaggtg
ctgaccactctgtgccacagcagccatgccctcggcaggcccctgacctgcagcgcgcct
cactcgtatgctctcccaggggcagagccatttattttacactgtaatgacccctaaaca
gtggattgggagggaaaccggctgactcagctcttgttactgcccgttttctcttaagcc
ctccccttggaatcaatagcacatgccattgatcacagaatgtctttttgttgctgttaa
cattgcaaaacataggttttgtgaaattgctggacatttggcgactgttctgtagggtac
agaatagggtttcctaaactatagcattgcaagtactttaccaaaaacacaacaaatctt
gtcttgggcactcactgctcatacccaggaaaattactgcagtagccacctagctggcct
ttctgcagctcccctcagcctccctggtctgttctcaacacagcaggcagagtgagcctt
ttgaaagcaaagctagatcatgtcactctgttcaggatcctgcagtggcttcccttttcc
cttgcgaggtcctttgcgacctgcccttccagtacatctctgaccttctgtctacctaca
gtatcccattcttaccctgcctcacccacctgacctggctgctgagtggagaaggcaggg
cctttgcacccccattcagcatttcctctgcctggcctgctctgctcctggctgtccaga
cggccaccttcctctcctgcctcaggtcttggctcaggtctcacacttactgtgagagac
cttgaagaaggaagggttactcctgcaactggcatgttccctgcagggaacctggaagtt
gtgactatggagtttgttacaagagctgttggcttctgtgaagaatgagacttttgagcc
ccggtgaccccacacaggaggttctcgcctcccgtgtctgtgccagactgctgcaggact
cccagcaagaggggcacctggcgctgccctgctggtgcttggtgttctgacctccgttct
tctgggtggccttggtgtgagtgtggcccatgaaagtaccatgggtctgagttgaaaaac
tgaaactggaggaagacccatagtggatgcttaggccttccatgaaattcctatttaaaa
ttgctacttacccacccgcagcccagctctgtctacccaaccctgctctgcttttctctt
gttcatagcacttattttctagtttattctatcacttcctaatttatgttattatttatt
gtctagttcccctgttagaatgttaattccacaaggacaagcatcttgtgcgtttcattc
attgtttgatctCAACTGCCTGTTAGCTTCCCaGgt
Primer
(+)TGACAAGACCTGTTTTCAAGGA
(+)caccGGGAAGCTAACAGGCAGTTG


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