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Human element [ hs2279 ]


Position: chr1:204,261,217-204,266,185 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: PLEKHA6(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TGTCCTGAATCCGCCTTTACtcttctcatggatttagatcaaataatgtagcagacctga
atacggtcttcatcaaaataaaggattatttgttctatgacagaagggaaaatatcaaat
aaaatcctttacggttcacaaagcactgtcatcattatctcatttggttcttttatggtc
ttatgaggtgggcagaaaagtgttagtaccaccctcattttacaaaacaaaatccctgaa
gctggaagtgttggctgagataacccacctcatatacctgaggcaaggctaacctcgggg
tctgttaacctcagtccgaagctctttcctcagggccacaaggagctgccccgacctggc
tgatgcccacctatgtccctcacccgggcactatgtccctcaccctggctgatacccacc
tgtgtccctcaccctggctgacgcccacctatgtccctcaccctggctgatgcccacctg
tgtccctcgccctggctgatgccccccatttccctcaccctggctgacgcctacctgtgt
ctcttgccctggcttggtctttgtggtgctcacccagcactgggtggatgctcctggagc
atgccagtggcagcacctgagaggctcctgggatggatggaaacagggtcaggacaggtc
aactaaggggagcactgcaagacagggaagactgatagaaatgagggatgcaaagtctaa
ttagatcacacatttgatgtgaagagggggaataaaaaacaaccaaatgccactcccaag
gcggaagtctggaggttgtggaagcagctggaagctgttagtgttagagagctcccggcc
agacacacggctgcgttccaggcctctgtacccctcccctcccgcctcttctctgtaccc
taaccttcctcctcctggcatggagccccacctctctcccccaacttgttctgagggtgg
gatgactgggagagggagcaagcccacactgtgggagtgggggagggagcacaaagttta
aggggcatccaaaaactcagtaatcagactttttttttttctttgagatggagtcttgct
ctgtcacccaggctgcagtgcagtggcgcgatcttggctcattgcaacctctgcctccca
agttcaagcgattatcctgcctcagcctcccaagtagctgggactacaggcatgtgccac
cacacccagttaatttttgtattttcagtagagatacggtttcaccatgctggccaggct
gatctcgaactcctggcctcaggtgatccgcccgccttggcctcccaaagtgctgggatt
acaagcatgagccactgcacccggccaatcaagacttattttaacgcgatatttttgaaa
taaaaattaacattaaaaacccatgatgaatgaaataccaacatttcaaataaagacagg
accagtatcactggaatttcccttttgcctcagcctccaatttggcttaaagtggcactg
ttttagatcctgtctttatttcgaatgttactattttgttcttcatgaaatgtttgcatt
gattttgattttaaaattattgcattgaaatgttatttatcttgactaccgagttttttg
gcacccatttggcaaaatgtaagacattttgtacctaaggggagtgtattaatcgcctta
ctctagtcagccctgctcagggatgaggaaagacgggacaaggctcaggaagtcccagag
aggatcctgtgctctttaagaaaacttcaatgcatgaacatggagaaaggctgagggact
ttccagactgagaggaaagagacatgatcctgaattggattctaatgacattattaggac
aactggtgaaatttcaatatggactgtatattagataatagaattgtatcgatgttatta
agtttcctgagtctgttaattgtactgtggttatgcaagagaatgtccttgtttttaaaa
ggaaaaagagaagatgaagaaagagtggccagagcgtgacagctgcttgacagctgcatt
aagccaaacacccttctctctctccagcctgtctctcctccaattccaggccatcccagg
aggatacggaggaagtggtgatggcagaaaatcttgtccagaagatagggtactggaggg
ctggttggacatttcttcttgaaataagaattaaccttgaattcccaaattacccttcac
cctcccaggaaaacttgacaactagcacagaaagaaaaaggaaattaacacctatccaag
tcatgatgctagcgaattctctgcttaacccccagagataggtattgctgtcctgctgtt
gcggaggaagaacctgagaggtcaggttattagcccaaggctacacagttaggaagtgag
agaactgggattagagcccaggacacagcccaaagccagctttcttcccactttatgtta
gttgtgcaacttgaaattcccttctcatgcatcatcctaccagctaatcctttcgtttat
ttttattgagttgaatccgcattagccttgagatttttggctctggagtcagactgcccc
agttccaatcccggctccaccagttactacctaagggactctgggtgagaaacttaacct
ctctgtgctttcattcccccatctgtgaaaacgggataatatacggaccttcttcatggg
ttcgtcacaggagtaaataagattatacccacaaaaaatttatgaaaggttctggcactt
agtaaatgttcaataaatgctaataatcattattatctgtctgcatctgccttgggctat
ttatccttaggagacagtggctcaattttgattataaaacccctcaccactttgacacct
acagatggatgcttaataatttgttcctaaagaagttatccatcaagatagtaccctctc
ccctgtacttaggagccagaacaattaacacctgagaatctcaaatgtgagggaggtctg
ttcctagtgtaatttgaactgggaggtgggcttttgtgtgtgtgtcatggttgaaagcag
tccattctaagggtcaacattactggacagaaataattgactaagaagacccagttctag
gccaggcgcattggctcatgcccataatcccagcactttgggaggccaaggtgggtggat
ttcctgaggtctggagttcaagtacagcctggccaacataatgaaaccctgtttctacta
aaaatacaaaaagtaaccaggtgtggtggcacacgcctgtagtcccagctactcgggagg
ctgaggcaagaggatcgcttgaatccgggaggcggaggttgcagtgagctgaggtcatgc
tattgcactctagcctgggtgacagagggagatgccatctcaaaaaaaaaaaaacaaaca
acaaaaaaaaaattctagtgcttatgctgctatccagctgcataaggtgtgtagcaagtc
cttgttctcttggggcccactagacctgagaaagggaatctcattgcccctagagcaaat
tataggacaaaagagctgctcatcctgagcaccttcggttcttcagggaagcgctccttc
caaaggctgcctgctcatctgaaaccctcaccccattttagaggcttagattcagctggg
gaggacccacagcccgagaaggaaaacaaaccaacagattatgcttctaggtggccacag
caggccaagccaagggcagggtggccaaaggtcaagcagcagtcagactgagccaacacg
agcctcctgtgacaaagccagagccagagcagggcaattttctaactataaacggaaact
caagaagctccaaaccgcacaaacacacgtgggaagatgagggtaaggcacagaagctac
ggggtgcacgcacacagaggaaggggcttcatgacgtgcaggcaggggaccaaggaaggg
catatgtgtcagtgtcgtggatgcagcaacagatctgggtttctggggtcctggagctta
tacaatttgtggtgggaggggcgtggtcctcattaaggaaaagactataggtacaaattt
gtgtgttagatatggccttggaaggggccagtgcaagtgcatcctcctccccatgtggat
ttctgaacactggtgcacactcacatgcgtgtgtttcgtggcctcctggttcccgtctca
gcactatttctctttcctctcacacagtgctttgcctgatttttttttttaatggtagag
agcatgaattaaagcccccacatcttggaattcaattcctctccaacacatgtgatgggc
atttagattttgcatgccttcccctaccctcctccaatgccctgcaccatcatcactatc
taccagagcagagtccctccccactcccctactcgctttgctgtgggtagccccaccttc
cccaaagggcattgcagcggccctctaagctgccagaagggtgaaaccacagctcccccc
agctgcctggccccttgcattccagccctgcacccagagtttgaccttctctgtggctgg
ctgcagacgtgctctgtgttacagaaatagtccagctcctgagcccctccaggcctccct
ctgcttcactcagagacacccccacctcactggctccaaaagagtcaggcaggatcagag
agaagtgctccgttctccagcctcctcccacacactactcagagcagagagtggggcctg
cctgttcccctccttctccttccagcctcagtactctgtgtctgcagagagcgtggacgg
aaatgagaggtttgaggcatcgcagagtgaaggggagcagaaagaaaaagcctgcagtac
catggaaagcttatgcaatttgacctgaagaaaggagttcagatttaggaacatggctat
taccaccataaccagagagccaccgTACCCATCCCTGCAGATTTCttTt
Primer
(+)TGTCCTGAATCCGCCTTTAC
(+)caccGAAATCTGCAGGGATGGGTA


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