Human element [ hs2227 ]Position: chr11:2,610,931-2,614,716 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: KCNQ1(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence CAACTTCTGCTGTTCCCAGAagtgcagctggctgagcacagacacagcaggtgacctgtg gtgatgccagggcatcagcgcttggcctccccacactgctccagggcagacctggcctcc tggctgcatcaggaggcacacgtgatgcatccgtcatctcgggaccagttttgagacaaa catcctatagggcaggaccgtgaactgggtagctctacagagtgtgaggggctggagacc tcctgcccactggccacggatgccacggatgcccttgagagttaggagaggtggaaggga ccccacttctccctgggagctccaacttctcttgcatgtgacttcagtgcccacttctgg ggactgtgatcccaaactgccaaattctctagcagctctcttaaaaccaaatgtgaagga ttctggctttttctgtcctctgagcccagcgtggggtatttgagatagctgtgctgccac caggacaggggcgtgcaccttgtcttcccaggagaaaacacagcaaagcctgtttgctcc ctagtacctcgtgcaaaggtgccatctgtggccctgctcgctgcttcctctgaggcagac atagtagtgactgtgttctgatacaaagatggcacttgcccgatgctgcactgcagcacc aatgcagaggccctggcaaatgttcgcagaagcccccgtcccagggcgtggtcccccttg gaggaaaagggcacctgttttgaaaacacttggttatcatggtgccagaaagcactagtt taggccccacatgaaacagtggccttgccctcccagcatgggagagccctcatcttccag gtggtggggggagcacagcatttgaagcccactcccctttgaccacttgctgttgtccag acagacatcacgcacgtgggccttgcattggttcctgcttactttgtctctgctgacccg gtttctgctccttgaagtctcagcccccttcttccatgattccaaggcatgtccctctaa ggaggcttgaccccaccctggagggcctgctccccactctctgccttccatgccccatgg gagggctccatgtggcccagtcccttggaggctggaccccactgttccggactctgggct gttggaagccaggccctgcctcagttgtgccaagtggccagggttctatgcctggcagag gaggtgtgggaggccaagcgctgatgccctggcatcaccacaggtcacctgctgtgtatg tgctcagccatccaagtaatatgatccattcccttctcagcagccgtccctttacccttt ggtcatttctgcatccatctctccctctcggacctcttcccagtgcctgggactaacccg aatgtctcagcttaccagcagttgtaaggatgctgtcacatcttatgcatggcagagagg ggacaaacccacagtgttcgcaaccctttgtgctcgcaggcaccagcccagcccgccaag ctccagttcattcaggatggctcacctgcatccccacctccttggcaggaggctgggtgg tgttccctgccccttggggctttcctgggcatcccatgtggaagtcagcaggcaagacca agtccaggctcccgctccactgggcaccaggctccccaggacttagatgccatcaggatt gggctgggacccgaccacatctcagtgagcccccgggacgtgctcccgcctgcccgctgg agccggcacaaagggcgattgtgccaaccaatgcccagtgcccccacagctccggattca gggctgagaccattgtctccatgcaaatgggggctttctgcgcatgctaacgggcaggtg ggcgtggccgggggctggggacccagcctgtgtgcggcctggagaagcatcgcttgctgc ttgctgaatgcggggcagcccgaatcgatgccgtccattcagcctggttagtgttatgaa gtcattagtgctgtctgcggaatgttaatgaggggcaagtgcggagaaagtcaggcgcct tttgtggaatgctcctccacggagaagatttctctttcctgctggagtttgttctttcct aacttgataagtcagcccaggctggaaccaccagctttctggagcctgagctgttggcct ggcccccagggacagagcgacccctgataatccggagtcccctttgcccaggaggctctt ggagagccctggcttggggagaaggagatgctgatcccagggcagatgccactcctgggg aagccccacagccccactcccgcaaggcgggtacgaacagccacactgagtcccccgcaa agtcaaacccaggcctcgaccttgtctggctgtgctttctgttgtgtgaacctgcaggtt aggcagaagtccccttgaccctgcctcagggctgctacctgtctgcgccatccacctgca cacaagccccttcagctcgtgctctagctggtgctgtgcggtgttggccccatttataga tggagaaacggaaggccagggccatgtggggaactcctgaggacacctgtgtgtcctgac acctcactgagcctgtcgagaggcacaggcaggtatggcagtggcagacctcaggggagg gaggctgggaaggtagaaggtcccccatggtagcatcagaacacagactaacctgcagca aaaatgggctgtgtggtccctgtagagcagcctcactgaggggaggcaccagccctcatc ccacgcagcagggcccgctgctgctccctcaaccttctgacctgggtggggagggagggg aatgtcagcagccacccagcccgagagccagagcacagcaggggctggccagcctagccc tagagtctttgcagtgaagcgtcacagactcctcatgaaggatgcatggggacccaaccg catgccacttggcttggcagtgtcaaagggactggggacctgcgaggatgcggtggccat tgtctgtgcccagcctggggagcctgacactcacagcccggctgctgctgccttgggcgt cctgaagatcctctgtatgcttgtggggtgggggcagacacacaggcagcctcctgccca gagggtccaagcgggcatggccatccacatctgcaggtcagaagaggcctgggctggagc cgcatactggccatgctgcctcgctgtccctgcctcagagccactgtattcactctgcct ggaactgtctttcctggatcccaggagtcctgaaggagccgcctccatcctgagctgcct accctgcccctcctccactccagttgtctccacagccaggtcaccattgaccaggttgtc cagtcccctcctcataggggagtggccctttgtctttcaccactgacccttcccaagctg gtaccatgcctaacccatggtgggtacacgataaaaatgcagggctgtgccaccagggtt ttgtcttgacaaagggactggaggcaggttgtcagagtagggctggccggaggtggcctc ctgaatgcccctaggaggccagaggagacttccccaaattcactggtcctggaggccgcc tcagaggccttttgctgctcagagaacactgggctggaaggtcgtggtctgtgacgtagg atcggggaggccacctcagaggccttttgctgctcagagaacactgggctgtaaggtcgt ggtctgtgacgtaggatcgggcagcacgcacctttccacctggcCTGGAGGTGATTCTGA AGGGctPrimer
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