Mouse element [ mm2216 ]Position: chr9:63,756,438-63,761,120 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Smad3 - Smad6 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence AGCAGATGTCTCCCAGGGATcctgctttggctacagcaagtcagtccttgaagcagctac tgggttcagcccttccttctgggcagccagaatcttcccacaggcagctcagtagatgga gttttgtaggtaccagctatctgcttgctgaaagggagactccagtacaggtctagccca ggctcctgtagaggaggggtagtcaacagtttggtaaagttgcaggagtcagcctgtagg gggaagtctggggagtagtggggagttctgtgttctgttttttgggggtttttttggttt ggttggtttggtttttgtttttttcggggttttttgtttgttttgttttgtttttgtttt tgagacagggtttctctgtgtagccctgactgtcctggaacttactctgtagaccaggct ggcctcgaactcagaaatccacctgcttctgcctcccaagtgctgggattaaagaagttc tgtgttcttgaaggaactaggaatggagccactacggagctgctgcttggcatccaaggg acatatacagggcattttcagctcgaggtaaagtctcaattttccacatcagggaaaatg atgtggacaaacccttcccacgggaagtgctgtggggactgaaggcaggagagggaaact ctggaagacagactgagcagcggcagaggttggaagaaagatgagcaactgaagtggcta ggagggcagagagaagtaggcagatgttaaaacatgcagagtcagaagtcaggaatccag tagagaggcaccaggtctagttattgatcagagcaatgctacacaagtgtatagcactgt ccccaaactggacatcccagaccagacagtgctaggattgcttttgttttaaatagctcc cacctttctgtccatgtttacagaaataactgagaattgaggaaactcttgtttcttgag cctgtcacataccacatccaccccatcatagtcacacacatgcacacacacacacacaca cacacacacacacacacacacacacactcaggagcagcaaggagcagcaccttaggtgca tgcccaatgttgaccaccagagggcgccgccaccatccagcaatgagctgtttaagaacc cgcccaagcaaggacagccaagcaacttagaaaacctgtctttctgcctcatttcatgtg ggtgggaaatgagactcaaaaggagaggcgtgagcaaagcaagcaaggaaggaaggagtg gccacttcaacagttggatacaagcagtgcagtttgaaagtagacactttaagcccagga atgaggcacctgccccaatgcaggcacacacttgagagttgagactatccttgcttgctg ttgccgtgatacaacactaatcaaaagcaacttgggggaagaaaagggtttattttgctt acaggttatagttaatcattaaaggaagccagagcaggagctcaagacaggcacatagag gcaagaactgaagcagagatcatggaagaaagctgcttactagccttatacagcagggac ccacctgcctagggatggtaccacccatagtgggcctggccctcttagtcagttatcaag caagaaaatgccctgtagacatgcccataagccaatctgatggaggccattcttgaattg aggattcttcttctcaggtgcgccaagatgatgactgatgctgaccatcacagccccacc tagacaaggaccttcttaccacctgtcaggtcccggcattcccactagggggatactctt agtcccagccatgcctcggcttttaaactctctctcaacctctcactctctctccatcct ttaaaccagcccacccaccttcctctgttttgtggctcaagtgagtccctgttccagtgt cctccaaagcagccccgcaagcttcttgctgcttacaccccccattggcatctgccaccc ttagagccagcaccagggccagaacagggagaccgaagcccaaactctgtccggccacca gctcttgtggtggtagggtgtcccactgccctctgcactgtcccttgagactaatagcct ggacatgcacacatcttgagtccacaatgctcttcctaatatcccttcaggaatagccct tccttctgtctttgtagggtgctttcagagcctaaaatgaagacatgtagctgctatgtg ttgcctagcaacaaaccagtgtgtagtccagcatgtacatgatgaacacatatacacaag ataagtaagatgttgaagtggaaacctaagggttctttggagagtcagttgtgttggatg gtgttttactggggcaaacacatgaaggagtgttttcctgaagtagacacaggtgaaagg ctaaggcagactcatgacttactcttctttaccaaagcagacaagatgctctctgaaagc aagcacgtgaaaggacacgtgatgaaggagtcttcgctaatgatgctcgcgcatggattg gtctgctttacgttgcattgttgagctccatttgtcaggactccatagagagaaacacac caaaaacttctggtggtgtgctgcagaaacttcttgccgcttccttggacttcttcatca ttttccctttggtcaagcacataggccattggaagatcatatgggtagttgggggaaccc tgggctggagtccagagcctcagacctggttaccatgcatgtcatcaagtgcctttccag acctcagtgactcatcagactgggacaagaatggccacccagtcaccgcatccaaaccac acttcacactcgcagtcacaaggaatatatccagaccccattccatacttgcccttacaa ggaacatacaagggacatgctacgatggcccctattttataggggaggaaactgtcgagg ggaggttaagtgagagagggaagacatgaccgtcatttcatttcacgaacagtaacatta tggcactcagagcgtggaggcctttcaagggatggctcagagggaaagagtgcgtgctac acacgcatgaggccctgagttcaaatccccactggcttgacctcatgggcacctgtattc ccagtgctgtagtggcagagacaggaagagcttgctggctgccaggctagctccaggcta ggtgagagatgatgtctcgagggaataagacagagagtgacagagcaggacagctagact ccgcacgtgcacatggatgggcacacgcgcacacacacacacacacacacgcacacgcac acgcacacgcacacgcacacgcacactatgcaaggctaggtgtggctcagcacttaggag gctaaggcatggcaagcttaggtcaggttgaggtgcataacaagattctaccttgaagga gagagaaggaaggagcacaaacaagatcctaaatactgtatcagacgattcacaggagac atgcaaatggtcctccaccctctgaaaggcttcctttaacacagcacaaataaaagaaat gcaaattcaaattgctccaagagaccttctctcgaatatcagattggcataaatccaaac gtttggcaactctccttcctgggaaggctgcagagaagcaggcactcttacagagagcga tgggaatgtggaagtggttctgctgggcgtgggggaggcagacgcagagctgttcttttc tttgcaattatggagactgaacccatggcctcgggcacacgacccaagccctctaccacg gagcatatccacagagatacatcagccaaatgctcctgggtcaacagaaagcccctaact gggctggcgagatggctcagcaggtaaaagcactgactgctcttccaaagatcctgagtt caaatcccacatggtggctcacaaccacccataatgagatctgactccctcttctggtgt gtctgaagtcagctacagtgttcttatgtacaataataaataaatctttggagttctgag caagcagggttgaccagagagagcagatgtcctaaaaattcaatttccaacaaccacatg aaggctcacaaccacctgtacagctacagtgtactcacataaaataaataaataaatctt aaaaaaagaaaagaaaaagaaagttcctaactgtggtcttatttgtagcagtagctagaa tcacatgagggacacccatgcgcaggaggcttggcggacaacacaagagaggactttgtg gctacaatggaactcagggccgggggtggcggcgggatgacaaccaagaaaacaagatat aggaaagtgtgactacaccttggtctaagaaaagggtcaggatgcgttttaacttgtgtg tgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtaagggcatgtgtgagtgtaagtccttc ccctggagctggagttatagagagttctgagtcacccaacaagggcactgaggatagaat tgggttctctggaagagcaacaagtgttcttaactgctaagccatttctccaatcctagc aattacactttatagataattaaattaatataagttgaagtaaggataaagtgtaaactt ttccattaaagtgatgaggaggattgggtaaaggggacgtaaaAAATGGGTCTCTTGGGC TGGPrimer
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