Mouse element [ mm2203 ]Position: chr9:63,733,907-63,738,653 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Smad3 - Smad6 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TCAGCTCCCGTGTGAAAGACaggccaaacacggtgcacacctgtaatccccatgctgggc aggaggaagcaggagaagcccggagccagccagctccagattcagggagagaccctgtct caaataacagtgtgaagaacgatagaatacactcactgaccccaggcctctacacacatg tacccatgtgtgtacatgcacattccacatccatagtcacacatgaacacatgcacacat aacaccacacacacacacacacacacacacacaatttaagagcacattttaagggtagcc aaaacccccatgattgaggcagagatatgaaaacagaatctggggttagtaaacaacgct tatgaaatggaaccctcaaataattattcttccagtctcaatcaagaaacccaaataatc tagcttctcagttcgctccagcagtttctttccctggacccctgctgctgatcagttcgg gtagcagctaatggagtctggcatgccaaaaccattcgttgtgtgttcttaggggcatcc ccagcttggaaatcacaaccaggaggtgacagcttccgaagcaccatcagtgcagggacg gggacatccttccagggagtcaccatcactggaagttcccccaaggatggagagtgtcag ggtctgaatctgaaatgtcgtccccccccccccccgccccaccacaggttcacgctttga ataattaattggtcctggtggccaggagatacttggcaggtagagagaggaggataagaa attctgtgtagtcctctagtcctctgccatatagtgagtttaaggctagcctgagctaca agaaagtctgagctatacaagatcctttctattaaaaaaaaaaaaaaaaaaaaagtggtc aggtaaggtcagaaggttatcagtcgcctcagtttgtcccaatagcctgtgctgatgact tctacccatgtccgtggatgtgcgtgtatgccacagccacagtgtatatagagaggtcaa agggcaacctcatgcattgtctctcaccttctaccttgcttgaagcagagtttcttgttc atggctatttgcacaaggcgagctggcccatgagctcccgaggaccctcaggcctccact ggccatctcattgacgtctcattgatggctgggaatgtagacacatgtgccacttttggg gttacgtggtttctggggatctgaactcaggtcctcaggcgtcctcagcaggccaagtac ctgctgagccatcccctagtccatactttgatgaccaggctgggccatttggccacctgg aaagaaaaagggggtctctaaggtgaaggacagtgagccatcttatcctcagggctgtgt ggtgactttcatcctgagtcaccagcagggcccttcccatctctgatgagtgacagctgc gtagtgttctgccctgaacctgctgtgcaatctcaggataggcacttccggcctctgggc catcctggggcaatcacacagtgctctctgcttaaagggcttagggacccatacgctacc ctcataatagtcaccatgacattggtcatgaattgtcctgggaggatacgtaggtcctgc aggggcatccagatgatgcctaccagaggagacactgactatgaaagatgagtagctgcc aggctctgggttagggtaagaagatggagacttacctatggggacaccaaactctcaagc cacaggattttaatacagtatttctcgtgtccgtgtacatgtgcgtgtacgtgtgtgtgt gtgtacatgtgtgggtgcatgtgtgtgtgcaggtatgaatgcatgcgcatgtcagtgtat ctccgagtagaggccagaagtcactatcaagtgccttcctcagcaactcctgcattttat ttttcctgctgaccctgggcctgctggccagtgagccccgccatctctccacctcctcag cactggagtaagagacacatatcaccatacctggcctttcgcctggatgatagggatcta agctcaggtcttcacacctgagcagcaagcacttcactaagtgcgctgtctccccagagc tctgatgtagtatgtcttctaaaaaaaaatcaaaatgaatgcccctgaaagctaatacaa atatcaaaattttagaaccaggtatggtgggtgggacataaccatggccccagaacttaa atgaaggcaggagaatcaggagtttaaggccatcttggctatagtaaactgaaggccaac ctaggctatatgagaccctgtctcaaaaacccatgccaaaatctaacaaaagcatctttt tgttgcgtttatttgtttgtttgtgtgttagtttagtgtgtgacacacacatacacatgc atgccagagcataagtatggagatcagagaatactttcaagagaccatggggtcccagga ctcgaactctggtagtcaggcttgctggcagaacctttatcagctgagccatctcaccag ctcaaaatttatttttaaataagtacaagctggatgctggcaaattttccttttgcctca gacgctaagacaatactttatttctcagatgggcctcttgctgaccctggacctgctggc ccagatatcttcctttctctgtctccttggcactgggataagagacacatgtcactgttt tgttaacaagacggcaaaggcacttgcccctgaacgtgatgacctgagtttgatccctgg gacccacaaggtggaaggagagaaccgactccctccagttgccctctgacctctacatga gcactgtggctcatactaaataaataaatgtaacttcatggtaactgggtgttttgtttt gagacatagtctacaaagtagcctctgctggcctcaaactcactgagatctgcctgcctt tgcctactgggtgctgggaattaatggtgtgtgccaccatacccagtgtaaagtgtaaag attttacaaagctagcacaggatatagcaacaacaaaacaaacaagcaaaatctgtgatt gaaattctctcacttacttgaatcatgggatttgaaaacacttgaagtattgctggcctt tgttcccctctcagggcctctcagcccttgggagtgacccctgcaggacacaagccaacc tgctcaggccagacatttgccacatctggggactcgggaggctggctgcacagtggacat gtgtgattttctagtccttggctgtgaggtgcttggattaaaggccctaggattctttta atatgaaaacaagctgtagccattaaaaatttagccctgcgaattcgtaactcctctccg aggtgctgtatttatttaatcatagtacattcgggccaagttctctcctggggctctggg tacctgtctccatgtccctgaggggcctgagctccagggccaagctgctcatacactccc ctgtctcccccacccccagccccagctcacagggagataaagcaccttagcttcattatt cagatggctaattgctaactgatttaattaaagtattcagatatttccccaagttagtta catttcatatcgctccgttaggagcaattggctggaatgaggagttgaggagagggcggg agagagggaggctggccaggtgggcggggactggtgtcccctggttggcagaggcttgct cactatttgaggcagcctgaggattgcctgactttctcctagagagcaactcacccgcac tcagaaacacactgagatcatgagcgtgtgtgtgtgagcatgtgtgtgtgtgtgtgtgtg tgtgtgtgtgtgtgtaagagaaagagagaggagaggttatgtgtatatgaatgatatgtg taagtgtatatgtatatgtgaaagtatgtatatgattgtgcatcatgtgaatatgtgtgt gagagtattgtgaatgtatatgtatgtgagtgtgtgtatgtgtgtgtgagaggggaggta tatatgaatgtatatgtatgtatatgttcatgtgaaaatgtatatgagtgcatgtataag tatgtgtgtgtgtgtgagaggggtgtatatgactgtatatgggtgttcatatgagtgtat atttgtgtgtatatatgtatatgaatgtatatgtgtgagtgtgcatagtgtaaaagtgtg tatatgaatgtatatggtatgtgtatgtgtgtgagtgtgtgaatgaatgtatacttgagt gtgtctatgagtgtgtgtgtgtctatacacttgagtgtggagcccagaggataatcttga ttgccactccccagcaccatgagacaggatcttgtactgatgtggacttgccaggtaggc tagctggctggtcagcttctgtccccaccactggggtcattagcatatgccagcacacct ggcttttttttaaatgtaggttctggggaatcaaacttcatgcttacaaagggaacactt caccagctgagcatcccagccccacccccagcctcacagaggttgttctcatcactaacc cctaatggacctaatggatgggttgaggacctgggactggagcccgttctggtacaaact gctctgagaccacagcaatccctaaaggacagcctccgtttctgtcaatgggatggaatg aagcaagtggagaatgcctgactacagccccttgcacagggctgcaaAGGCCGAGGACTA CTTAGCAPrimer
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