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Human element [ hs2196 ]


Position: chr7:151,408,696-151,411,333 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: PRKAG2(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AATCTCCATCCACCAAGGAGgacagcaatattatccccattttatggatggtgaaactga
ggcacaggaaagttaagcaacctgtagatatttatcaaatgtgttgaaggaacgaggaag
agaacaggctctccatcacagaagtgtttaaactacttgttagggttactctcggatatg
caggttcctgagagccgctagcctgtctcaactcagatgcaagttctacaatttagaaat
ctttggaccccaggactcttcctagccacagcctggtgctaactctagacatggggacac
aggtccagtgttggtggcctgccaccgactgggtttggcaccatcacccagaagccgccc
tcctctgggtttcccaggcgccccaggtggctgggctgtgtctcgggtgatggaggaggt
gtacagatcccatgtcagggtgtgactgcccggaggcagtggcggcagaatgtcaggtgc
caccagggactccaggccttcctccgcggcgcgtacgtgcatcacgtggtgtaacatgac
tgggcggctcagtgtggcccccaccctcacgtgccctcctggcacccagcctccgcccga
ccctctgagagctctgaggagtgtgaacaatgggacgggctcaggctcactcacccggga
aggacgctctgctggctcagcaggaaggggcctggcacccaaagcctggacatcctgtgt
ggcctggagggagaatcagatggggtggccaccgcaggagactgggtccctctcctccac
tccctggcagtgactctacactcaggacccacgagactgctaatggtaaccacagggaca
gccctgcaggggccttgctctggcccactgtgaaagggaaagtccccttcctccactgat
gtaacaaaacaaatctgctcttgttggccctgatgagctgacacccggccagagccaagc
ccagggatgggaggtgcccactggcctgacctcggacaccagctcctgggctgcaggtcc
aggaggcacccaggcacaatcaggctcctgttgccaaaaaagggagtgcatgcagagtaa
gcaaaatgagtgtgccccataaccacattcagtcacccggggccacgctgtgctgaggga
gggtgtggaacacaccacaatcctctccagcctccatctctgtctttcctgggagaggtt
ctgaggcctcgtggagatttacacaaagcctgatgcttcagaggggaaaatgctctggcc
atgcaaattcattccgttttccatcactcattttgggtgcaggatattttcagcactttc
ccagcatgcaaagcaaagacaggattgtcatggagagccctgtgtgcttggaacgtgcca
agagcagaggggcgtgagctggtttggggcattctctctgcttccctacaaatgcccagt
ggatggcccttcctcggagctgaagcagttcccatttctcctgtatggcactgtcaaatc
gaggcagagggacgggaaatggtgaacagagaaggaagactcaagggcaaaactcccgga
aggacagggggtcatggaagaaggaattctgagcctgacatcttagaaaatggatatgca
gtttgcagatctacatccctcacactgtcttctgggaatggcgagaattgtggactttgt
ttccttttgctctgatatctttccccccacccaaagcagacccttttgtttaattctgat
gctgtaggacgcaccccggcttgcaagccaggctgtgaatcagcggtttttgaaaaggat
atggtaccacagctcacacatgtatgtaatgcagtattttctttttttacttttttagta
tggaaaaatttaaacacatatgtaaaagtagacagaacagaataatgaactccatttttt
ctagcatgccatttcaacaactattaactcatgaggatggtgtttttgcttttttaattt
tttttttttttttgagacaaagtctcgctctgttgcccaggccggagtgcagtggtgcga
acacagctcactacaacctcaggaggctcaagtgaccctcctgcctcagcttcctgagta
gctgggattacaggtgcaggccaccacacccagttaatttttaaatgtttcatagagaag
gggtctatgttgcccaggctggccttgaacttctggcctgaagtgatcttcctacctcgg
cctcccaaagtgccaggattacaggcatgagccactgcacctgacccccagtgtttaaat
gtagctaccatgacgtatgcatcaaaatcacctacaagtcttatgaaaaatgcaaatttc
ctggatctcaccccagatcaaatatatcacaatttgggggttcaagtccaagaaccggca
catacccccaaaggattctcacagacagtcaagtgtggggggtcactggtagcggttaga
taactcgggctggggtcccagccctgccacttactagctaggtagctgaacaggtgatct
ccattggagtacgggctttacggggttgtcatggggaataaacaagatctcacaagaaga
gcacctagcacagagtcagcgctcattaaacatcctatGCAGTTACTCACCGGCCTAA
Primer
(+)AATCTCCATCCACCAAGGAG
(+)TTAGGCCGGTGAGTAACTGC


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