Mouse element [ mm2191 ]Position: chr13:15,326,333-15,331,630 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: AW209491 - Gli3 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GCAAAGAAAGAGAGACCTTGGAatgctcaactcaaaaatagatgtctccataaaatccct cctctaagtgatagggaattctgtagaagaggaggcagaaactatgtaaaagccagaggg gttggaggacactatggaatcaaggttttgtagatacaatagggctggtgcacatttgat ataatagggctggtgcacagagactgaaatagcatgcacaggtccagcatggttctacac taaatggggtcctagagcttaaaggagaagtggacatatgcccccctcctgaacccagaa actatttctaattgatagtcacatttaaatgaaaaatttgttttctccaagtaagtctca caagggaaacaaacaattcttaagtgtaggactcatgcctagcagtagttggccaacata aaatgaacccactggcatttttggaggttcttattttcatgattttatcagggcatttta attttgtacctttcatgtcctttgcatttatactatgacttctagtttggatttttgtca ggctactctgtgtgtgaatctgtgtgtccctgaatctatgattttcttgtgccactgcct cctcctcctcctcctcctcctcctcctcctcctcctcctcctcctcctccttctctcttt atggctgttagcttgatgctttgtgggactagcagcgggaataggtgtacctttaactct tttgcctgctcatggtacttttttcctgtactgggttccattatccagcctcaatatgag agtgtttgcctcatcttaatataacttgttttctcttgtttggctgtcatatattagagg cctactcttttctgaagaagaaatggagggtaatagatctggggaagaggagagctgtag aggggctaaaaggagtggaggagggggaaactgtggtcaggatatcttatatgagagatg aatgtatgtttttttttaaaaaaaaaacctacaaaaaaacacaaagaaaaaagaaagaaa ataaattcttttctcatacaatatattcagatttcagtttctcctccatatactcctcca agattctccccacttcaacaacccatcatatccactgttctgtgtctctcagcagaaaag aacaagcttctctagagactttgaagcaatgacttatttcaatgagcattcacaagtgaa gatatgtggacaaagggatacattgtgggacacactgtgacacactacagcttccacaat gagatatttcccatgctttgttttgttttgtttgtgcatgagcatacatatgtgtgtgtg tgtgtgtgtgcatgcacatatgtgtgtgtgcatatgtgtatatatgtgtgttttattttg ggaggaatgttgcaagggcaagggacaaatatgaggagaatggaaaatgatcaaaacggg ggtacatcatatgaaacacaaaaaatcaataaaacatttttaaaaactggattctaatat atatcaatgaatcatagcaaaataaaatataataagataaaacaaaagctagcacatcca agccctcagcttctgtagagcaaaggacactgctattcaaagaagcatcctaaataatgg attttttttttacctgttccacatccaacagatgactaatattcaacatatgtaaaaaac tcaagaaactagatatcaaaattccaaataatccaattaaataatggggtacaattttaa acagaattctcaatagagaaatcttaaatggctgaggaacactaaaagaaatgttccaca tctttaaccatcagggaaatccaaatcaaaactactatgtgattacattttacacctgtc agaatgactaagatgaatagcacaagtgacagttcctgctggtgaaaatgtgggacaagt agaacactgctcccttgctggtaggaatacaaacttgtacacagccattatggaaattaa tatggcatttcctcagaaaactgggaatcactctacctcaagattcagctatatcagctt agggcatatagccaaaagatgcttcatcccactatgatgacatttgctcatgttcattgg tgctctattcataatttcaaggaactagaaacaacctagattgttcttcctcaacagaag aatggataaagaaagtgtggtacagggtcgccatcttggttccgggactcagcagaactt aggaaattagtccgaacaggttagagggtgcgccagagaaccagacagcttctgggacag gcggaagcacagagccgctgaggcagcacccttggcgggccacagacagctggccaccgt ctggaccagaggacaggtgtccacctggctcgggaggcagcctcagcctcagcaacagcg gtcgccatcttggttccgggactccgccaaacttaggaatttagtctgcacaggtgagag tctgcaccacagaagctgacagcttctgggaactgccaaagcaacacagcttctgagaaa ggccctgttttggggcttcttcttcgaccaggaggaggtccaaaaacaagatatctgcgc accttccctgtaagagagcttgccagcagagagtgctctgagcactgaaactcagaggag agaatctgtctcccaggtctgctgggagacggtaacagaatcaccagaagaacaatctct aaacagagtcaactataacaactaattccagagattaccagatggcaaaaggtaaatgta ggaatcttactaacagaaaccaagaccactcaccatcatcagaacccagcactcccactt cgcccagtccagggcaccccaacacacccgaaaagctagacctggatttaaaagcatatc tcatgatgatggtagaggacatcaagaaggactttaataactcacttaaagaaatacagg agaacactactaaagagttacaagtccttaaagaaaaacaggaaaacacaatcaaacagg tagaagtccttacagaaaaagaggaaaagacatacaaacaggtgatggaaatgaacaaaa ccatactagacctaaaaagggaagtagacacaataaagaaaactcaaagtgaggcaacac tggagatagaaaccctaggaaagaaatctggaaccatagatttgagcatcagcaacagaa tacaagagatggaagagagaatctcaggtgcagaagattccatagagaacatcagcacaa caatcaaagaaaatgcaaaatgcaaaaagatcctaactcaaaatatccaggaaatccagg acacaatgagaagaccaaacctacggatataggagtggatgagaatgaagattttcaact caaaggaccagcaaacatcttcaacaaaattattgaagaatacttcccaaatataaagaa agagatgcatatgaacatacaagaagcctacagaactccaaatagactggaccagaaaag aaattcctcccgacacataataatcagaacaacaaatgcactaaataaagatagaatact aaaagcagtaagggaaaaaggtcaagtaacatataaaggcaagcctatcagaattacacc agatttttcaccagagactatgaaagccagaagagcctggacagatgttatacagacact aagagaacacaaattccagccaaggctactatacccagccaaactctcaattaccataga tggagaaaccaaagtattccacgacaaaactaaattcacccattatctctccacgaatcc agcccttcaaaggataataacagaaaaaaaccaatacaagaatgggaacaacgccctaga aaaaacaagaaggtaattcctcaacaaacctaaaagaagacagccacaagaacagaatgc caactttaacaacaaaaataacaggaagcaacaattacttttccttaatatctcttaaca tcaatggtctcaactccccaataaaaagacatagactaacaaactggctacacaaacaag acccaacattttgctgcttacaggagacacatctcagagaaaaagatagacactacctca gaataaaaggctggaaaacaattttccaagcaaatggtatgaagaaacaagctggagtag ccatcctaatatctgataagattgacttccaacccaaagtcatcaaaaaagacaaggagg ggcacttcgttatcatcaaaggtaaaatcctccaagaggaactctcaattctgaatatct atgctccaaatactagggcagccacattcattaaagaaactttagtaaagctcaaagcac acattgcacctcacacaataatagtgggagacttcaacacaccactttcaccaatggaca gatcatggaaacagaaactaaacagggacacactgaaactaacaaaagtgatgaaacaaa tggatctgacagatatctacagaacattttatcctaaaacaaaaggatataccttcttct cagcacctcatggtaccttctccaaaattgaccacataataggtcacaaaacaggcctca acagattcaaaaatattgaaattgtcccatgtatcctatcagatcacaatgcactaaggc tgatcttcaataacaaaaaaaaaaaaaaaaataacagaaagccaacactcacgtggaaac tgaacaacactcttctcaatgataccttggtcaaggaaggaataaagaaagaaattaaag actttttagagtttaatgaaaatgaagccacaacgtacccaaacttatgggacacaatga aagcatttctaagagggaaactcatagctctgagtgcctccaagaaaaaacgggagagag cacatactagcagcttgacaacacatctaaaagctctagaaaaaaaggaagcaaattcac ccaagaggagtagacggcaggaaataatcaaactcaggggtgaaatcaaccaagtggaaa caagaagaactattcaaagaattaaccaaacgaggagttggttctttgagaaaatcaaca agatagataaacccttagctagactcactagagggcacagagacaaaatcctaattaaca aaatcagaactgaaaagggagacataacaacagatcctgaagaaatccaaaacaccatca gatcattctacaaaaggctatactcaacaaaactggaaaacctggacgaaatggacaaat ttctggacagataccaggtaccaaagttgaatcaggatcaagttgaccttctaaacAGTC CCATATCCCCTAAGGAAAPrimer
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