Mouse element [ mm2174 ]Position: chr13:14,855,687-14,861,144 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: AW209491 - Gli3 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence ATTTCCCCTCTCATGGGACTACtgtattgacaaggctagttcttctcctaaggaaatgta ttccttttcatttgtttgtttggtgtttttccttttatagaaaatagaatttggatcttg gtccgagacccgccgaaattaggaaattagtctgaacaggtgagagggtgcgccagagaa cctgacagcttctggaacaggcagaagcacagaggcgctgaggcagcaccctgtgtgggc cggggacagccggccaccttccggaccggaggacaggtgcccacccggcaggggaggcgg cctaagccacagcagcagcggtcgccatcttggtcccgggactccaaggaacttaggaat ttagtctgcttaggtgagagtctgtaccacctgggaactgccaaagcaacacagtgtctg agaaaggtcctgttttgggccttcttcttcggccaggaggaggtccaaatacaagatatc tgcgcaccttccctgtaagagagcttgccagcagagagtgctctgagcactgaaactcag aggagagaatctgtctcccaggtctgctgatagacggtaacagaatcaccagaagaacaa tctctaaacagagtcaactataactactaactccagagattaccagatggcgaaaggtaa acggaggaatcttactaacaggaaccaagaccactcaccatcaccagaacccagcacacc cacttcgtccagtccagggaaccccaacacacctgagaacctagacctagatttaaaagc atatctcatgatgatggtagaggacatcaagaaggactttaataaatcacttaaagaaat acaggagaacactgctaaagagttacaagtccttaaagaaaaacaggaaaacacaatcaa acaggtagaagtccttacagaaaaagaggaaaaaacatacaaacaggtgatggaaatgaa caaaaccatactagacctaaaaagggaagtagacactataaagaaaactcaaagcgaggc aacactagagatagaaaccctaggaaagaaatctggaaccatagatttgagcatcagcaa cagaatacaagagatggaagagagaatctcaggtgcagaagattccatagagaacatcgg cacaacaatcaaagaaaatggaaaatgcaaaaagatcctaactcaaaatatccaggaaat ccaggacacaataagaagaccaaacgtacggataataggagtggatgagaatgaagattt tcaactcaaaggttcagcaaacatcttcaacaaaattattgaagaaaacttcccaaatct aaagaatgagatgcatatgaacatacaagaagcctacagaactccaaatagactggacca gaaaagaaattcctcccgacacataataatcagaacatcaaatgcactaaataaagatag aatactaaaagcagtaagggaaaaaggtcaagtaacatataaaggcaagcctatcagaat tacaccagatttttcaccagagactatgaaagccagaagagcctggacagatgttataca gatactaagagaacacaaactgcagcccaggctactatacccagccaaactctcaattat catagagggagaaaccaaagtattccacgacaaaaccaaattcacgcattatatctccac gaatccagcccttcaaaggataataacagaaaaaaaccaatacaagaacgggaacaacgc cctagaaaaaacaagaaggtaatccctcaacaaacctaaaagaagacagccacaagaaca gaatgccacctttaacaactaaaataacaggaagcaacaattacttttccttaatatctc ttaacatcaatggtctcaactcgccaataaaaagacatagactaacaaactggctacaca aacaagacccaacattttgctgcttacaggaaactcatctcagagaaaaagatagacact acctcagaatgaaaggctggaaaacaattttccaagcaaatggtatgaagaaacaagcag gagtagccatctaaatatctgataagattgacttccaacccaaagtcatcaaaaaagaca aggagggacacttcattctcatcaaaggtaaaatcctccaagaggaactctcaattctga atatctatgctccaaatacaagagcagccacattcactaaagaaactttagtaaagctca aagcacacattgcgcctcacacaataatagtgggagacttcaacacaccactttcaccaa tggacagatcatggaaacagaaactaaacagggacacactgaaactaacagaagtgatga aacaaatggatctgacagatatctacagaacattttaccctaaaacaaaaggatatacct tcttctcagcacctcatggtaccttctccaaaattgaccacataataggtcacaaatcag gcctcaacagattcaaaaatattgaaattgtcccatgtatcctatcagatcacaatgcac taaggctgatcttcaataacaaaataaataacagaaagccaacattcacatggaaactga acaacactcttctcaatgataccttggtcaaggaaggaataaagaaagaaattaaagact ttttagagtttaatgaaaatgaagccacaacgtacccaaacctttgggacacaatgaaag catttctaagagggaaactcatagctatgagtgccttcaagaaaaaacgggagagagcac atactagcagcttgacaacacatctaaaagctctagaaaaaaaggaagcaaattcaccca agaggagtagacggcaggaaataatcaaactcaggggtgaaatcaaccaagtggaaacaa gaagaactattcaaagaattaaccaaacgaggagttggttctttgagaaaatcaacaaga tagataaacccttagctagactcactaaagggcacagggacaaaatcctaattaacaaaa tcagaaatgaaaagggagacataacaacagatcctgaagaaatccaaaacaccatcagat ccttctacaaaaggctatactcaacaaaactggaaaacctggacgaaatggacaaatttc tggacagataccaggtaccaaagttgaatcaggatcaagttgaccttctaaacagtccca tatcccctaaagaaatagaagcagttattaatagtctcccagccaaaaaaagcccaggac cagacgggtttagtgcagagttctatcagaccttcaaagaagatctaactccagttctgc acaaactttttcacaagatagaagtagaaggtactctacccaactcattttatgaagcca ctattactctgatacctaaaccacagaaagatccaacaaagatagagaacttcagaccaa tttctcttatgaacatcgatgcaaaaatccttaataaaattctcgctaaccgaatccaag aacacattaaagcaatcatccatcctgaccaagtaggttttattccagggatgcagggat ggtttaatatatgaaaatccatcaatgtaatccattatataaacaaactcaaagacaaaa accacatgatcatctggttagatgcagaaaaagcatttgacaagatccaacacccattca tgataaaagttctggaaagatcaggaattcaaggccaatacctaaacatgataaaagcaa tctacagcaaaccagtagccaacatcaaagtaaatggagagaagctggaagcaatcccac taaaatcagggactagacaaggctgcccactttctccctaccttttcaacatagtacttg aagtattagccagagcaattcgacaacaaaaggagatcaaggggatacaaattggaaaag aggaagtcaaaatatcactttttgcagatgatatgatagtatatataagtgaccctaaaa attccaccagagaactcctaaacctgataaacagcttcggtgaagtagctggatataaaa ttaactcaaacaagtcaatggcctttctctacacaaagaataaacaggctgagaaagaaa ttagggaaacaacacccttctcaatagccacaaataatataaaatatctcggcgtgactc taacgaaggaagtgaaagatctgtatgataaaaacttcaagtccctgaagaaagaaatta aagaagatctcagaagatggaaagatctcccatgctcatggattggcaggaccaacattg taaaaatggctatcttgccaaaagcaatctacagattcaatgcaatccccattaaaattc caactcaattcttcaacgaattagaaggagcaatttgcaaattcatctggaataacaaaa aaccgaggatagcaaaaactcttctcaaggataaaagaacctctggtggaatcaccatgc ctgacctaaagctttactacagagcaattgtgataaaaactgcatggtactggtatagag acagacaagtggaccaatggaatagaattgaagacccagaaatgaacccacacacctatg gtcacttgatcttcgacaagggagccaaaaccatccagtggaagaaagacagcattttca acaattggtgctggcacaactggttgttatcatgtagaagaatgcgaatcgatccatact tatctccttgtactaaggtcaaatctaagtggatcaaggaacttcacataaaaccagaga cactgaaacttatagaggagaaagtggggaaaagccttgaagatatgggcacaggggaaa aattcctgaacagaacagcaatggcttgtgctgtaagatcgagaattgacaaatgggacc taatgaaactccaaagtttctgcaaggcaaaagacactgtctataagacaaaaagaccac caacagactgggaaaggatctttacctatcctaaatcagataggggactaatatccaaca tatataaagaactcaagaaggtggacctcagaaaatcaaataacccccttaaaaaatggg gctcagaactgaacaaagaattctcacctgaggaataccgaatggcagagaagcacctga aaaaatgttcaacatccttaatcatcagggaaatgcaaatcaaaacaaccctgagattcc acctcacaccagtgagaatggctaagatcaaaaattcaggtgacagcagatgctggcgag gatgtggagaaagaggaacactcctccattgttggtgggattgcaggcttgtacaaccac tctggaaatcagtctggcggttcctcagaaaattggacatagtactaccggaggatccag caatacctctcctgggcatatatccagaagaagccccaactggtaagaaggacacatgct ccactatgttcatagcagccttatttataatagccaGAAACTGGAAAGAACCCAGATGPrimer
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