Human element [ hs2171 ]Position: chr2:71,728,919-71,733,874 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: DYSF(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TGTGCCTCTGTTGATGGAGActtggtttgcttacacctcttggctattgtgaataatact gctgtgaacacaggtgtgcaaatatctaggtccctgatttcaatttttttgggggtatat aactagaagtggaattgctagatcatatgctaattctaggtttaattttttgagtaactg ctatgctgtttcccacagtggctgcaccgtttacgtccctacacaccatgcacaagcatt ccagtttctccatatcctggccaacacttgtgatttttctctccatgactctgtgtgtgt gtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtttataatagccatcctaatggatgtg aagtagtatctcagtgtgagtagtcagttttaaaaatcacctcgttaggtcgaggttgca gttttatttgatagggtcataaaaatctatattccaatcaatatttgcagagaccagaaa agggatgagcccagatgacaggtgtgctgttcatccatcaggaaaaagcagttaataagt acacctgtggctgttcaccagcctaacatggccttgtgccctgacagtaatgttcagggt aagtccatttctcagggaggcaagcagaagtcacacacagacaccaggcgacaggttcct acagtcacaggccactcactgcccatggtttcctgctgcctctacagaggggcagactat atggtgagcccacttcatagcttcctctgctctctttgaagggagcatgtgccaaccatc gcgctgctgacctactctttcctaatagcccatacatgtgccccacccctcctgtcagtc agaaatgcagcctctggtgcgtgtggtatggaccgcccagggcctctcaggagtggagcc tgtttcccctgccccaatatccttttctccctgtccctgaagacaggaaggtttccttgc ttttcatgtacaatcaatcactgccggcggagccctgggattccaatacagacggcctgt gtagacatgggtcatatgatctgctggggctcaggggcagtgtgggggtgatgagatact gacctggtggactgtcaagggctcatgggaagacaacccttcatctctggggaggcgtgg ctgggcctctggggaggggcaggaaggggtcaaggtggaccttggagaaggaaaggtatc tcttatcccttcccatgcccaagtatttccctaggggccaggccttccagggatggtgca tggtggtgactgggtgtgggggtgcaggagagccctcgtggggtgctgggtgactgtgtg gtctaggcagaccagcctcatctgagtggtggcagtgaggggtctggcagaagagccagg gtgccttaggctagttttctacatttgacttctctctcctctcaggttcctgggggaagc caaggtcccactccgagaggtcctcgccacccctagtctgtccgccagcttcaatgcccc cctgctggacaccaagaagcagcccacaggggtaagtgcccatcagcctctgccaggtta aggtccaaggcattgccaggtggcttcctctttgggccttgccattctgaccccagacat gcatctgacttcagctcccttgaagcaggcctggccctccctgaccagagtttgcagtgt ctggaggggcactggactaggggagatgggaagggcagagggagccctgaccccacctgc agggctcaagtcagccttggctcgcagcagcttggcctgccccttggtctggtgctgccg gtctggccacctcctgtccccagcttcctggtcagccttggctatagcccacctagtctc cctggcagacctcccctcctccctcaccatccagccctcttctccagattcagaatctct gaaccatctgggtcacagggcatgagggatggagggaaagagccctctcccctgcccacc aggaccctcttccttttcctccgttgttcttcctgcccctccctggccttctcagtgctc cccccaagtccaatcagacaattcccagccctcctctctgcctgttatttttagtgagct aaatataccctgtagggttctctcttcttggtagaattcctgacctctcctgtgggttag ccccgccttccaggatgtaccaggaaccatggattggaactacttccgccctgtgaggcc tgggggattcaggccctgacttgtctcagacagggcagtggtactgccccgtggcgggtg tgtgaggccggggctcctggctggctgggtgagtcaggaagtagccggtctgggcagatc tctgaatggaacgagactggatgctgagacctagagaaaaggaaccactgaagtcacata gtcagtggctgttcccatgggggcctggaccaggagctcatcccctagaccctctggtgg aatggctgtggatggactcctggttcactgaactctcagtctgctgccctctcggtgctg gactttgctcccaaggggtcttaggctggggatggctgggctgctcccttgatggaagat gtcacttcccaggaaagtccatctgtttcccacacagggctcactggcagaatgttctgg tttagagcagtattcactgagcctgtctccatcaccctgagtcccctctcatcctccgtt tcagcctcctgtctcttaacactccctgtggtcaaggaggaccccaaggagttctggaac taccccttcaggggtcctgaggaggctcagacccaaccctcaaggcatgggacacagatg gagggatgataggtatcaaggcagctggcgagagaaagccttggggtcaggcttggttct cacttccttgctgggacaccagcatctctccactccagctctgtgtgcatccatgactct ggtgtttcctgcccttcggtcccttccaacctcaaaggcctgtccagccccgaggcagag ttttccatagctggtccctattcatgagccgtgtcaggctggagccatcccctccatacc tcccttcgcctcctcctgctccttgttcccacactgtctgggcttcatgtttaccagcgg ggagcctgctgccttctcttccctggctggggattcctggctgtctgcagagctttacaa tccttcaccaaccgtgtctccaaaaagagtgtgtgagatccaggaggtggcggatgggag agagtgctggaccctgctgggttgagccccactagctgagcacaggctggaaatggagtt ggggagcctcctccctgttgcccaggaggcagtcctcacctgtacagagggtctaggact agattctagtccatgtgatggcattaaggtcacagtgcagccccagacaggtcatatgtc cctttgggctggggcttcctgcctgggacctgggctggctgcactgtagtgcgaggcagg gataactgggggtgtgctgggcagggacagacagccaacaacattgagggttctcgctgg ccgctcactgagacctcatttctgagctaagcccaatcagcagagcatgccaggtgcccc catttcattgtagctctcagttgcctctaagggccaaagaacctgctgatggggtgatac gagtgaacacagagccagtgggatccttaggaaactctggcagactgagaggcgtggggg aggccgggagtcctgccgcaggctgactgcagaccccgtttgccttttgctcagtgttct cacccaagtcccttcagatatagcacgctgggctgtgtgccatggaggatctggctatgt gccaaatctaccctctgacagctgctaggttgatggggaaaacactcccccagtcctgga ggaacctccaggccatggggatttaggacacacatggaggaaaagaagcagcatcctctc agatgtattttaagaggagctcactcaggaaggtgtggatacatggagaaaactcagtcg gcatcacccgggccttaaagccagaatcggatcacaaaggaggacaagagcctacgaagc ccagtggtgtgtgggtcagggaagccaggagggcactgtgtgttgggagcggggacctga ggcgggcaggacagtgctcagtgagtccctgagctttcttcaaaggtttggagcttctgc caaccaaccctgtatcagctcagggccacagctaccctctgcctgcttctggaggttgtc tgagggagaggttagtgagggccacgcacatatcaggcctgtcttgtccacacgggacac atgtgttaggggaggtgagagtgggctggtctgggttttggttttgatgagctcagtgtt ggagagctgtggtcgaggaagtaggtggcgcacaatgggctgggcatggcagggtgggcg aaggaccccagagcctgaccacataacctggaggagggtcacagccagggagggtctgag gaggtcattcgccttcagtttcctgagccctcttagcctggggcaagacaggagtctcaa gttctgctgatctcgttgattgtgggccccaaggcagcagacgagggcgaccgaggttac cctgctccagggcagcgtgtttggtggacacacgattgactgacaatggccttgtgcgtg gagaaccctggagtgctgctgagttggagactgaggacctgggctgtcaggcgcgggacc cgtgacgtggaggaccttgaggaaggtcccactgggagctctaggcccaggttgtgtagt ccgggagagcacagggctcacatatatcccccaggaagaaaatcttagctctatttgtct agagaaggagacaggggttgagctgtgatgtcatggtgagggtacaaggtcctggtcctg ggtggggcccactgacACGCCCACTTTCCCTTAAGAPrimer
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