Human element [ hs2147 ]Position: chr11:2,552,386-2,556,805 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: KCNQ1(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GCAGGGACCTTCAGACGTGccctgggctccacatgcccgtctgcagctcgagaattagac gtgccctgggctccacatgcccgtctgcagctcgagaattagacgtgccctgggctccac atgcccgtctgcagctcgagaattagacgtgccctgggcttcatgtgcccgtctgcagcc ctagaatcaccagcagcaccccaaggcccccatgcggcatctaaaccaggcaggggacag gggcatggagcagaccaggtagggttgctccctaggatgcagccctgcccaggcaggagg gcagaccctacacagtgctgagctgtgcccccagggaagcctccaagatggtcctcatcc cagcagccctgcccactgtctgcgcatccctggggtctgtgcgccacctggtggctattt gctgaactgtggccacaaacccctggtcagcaggaaccggccctttcactgccctgcagc tccctctcctccggggtggcatggcagagaagcagcccgtggccccaagcatagagcccc ctttctgctgtagtcccccttgcagtcccctctgccttttccactgcttggaaggcagag agaccctggccgcctcccttgtccctttatctgtcatgccagctgcctccctgccacacc ttgcgtctcaccaggcaaccctgcaaacagcagctggggaggactttctaagacaaaaac ctgatcattccagaagctccctgtgacgtttaggctacattccccaaggtggtgccacgt tcaggcccatcacctgtaacactacccaccccacctcacccagcctgggctcactcactc caggccaagccttggacattcctggctgccccagggcctttgcaagccctcttctctgga cctggctgtcctcgggagtgtcagcactcagcctcttgtcaggtttcaggcctctgcttg gcactatcctgcacaccatccccgccgacctgccctgttctgggttcatggggctctggg aggctcacaagcatctgtctgcctcaggccattccacactccctgagggtgggaccccgt ggctgcgctaaccccacaagctctgcatggcgcttcctggccgtgcatgtccctccctgc atcaaatgcataggccagagttgcccagcgaggatgacccagcttggtatgtgctcccgg cttcagctgcccctgcgtgatggagacaccctcccactcgccttgccacacttcccagca gtctctcgaagcactggccgcgtggaaacaaatccttcaaggggatttgagggtccagct gtcactcagtcatggccatggtgggggcacgttggggacacagcagagagtgaggcagcc gtgggcctggccttgacaggcggatgtacagacacatcagggccgagactgaggcgtggg ctctgggtggggagacagtgaccacttgcttggaggagggggcgctcaggtgggaggggc acagtgagaaggaggtaagctggtgctggcggggcaggcagggacccctcccagggctca cagcttccagcggacagcaggcacagggctgtggggatcagagcacaggcagaagtcacc tgacagcaggttcccgaggcttcaggcctggaggagtgaggacggctgttgggttcttga ttccagcagagggaggctgtgcagggttgcaggaaggacggttgggggcgcttcggggag gtacgagaagcaaaggtgtttgcagcccttggggccaggcggcgggaaggcctgtagacg ccagagggcgctcgtgctacatgcaactcaggagctgctggcacccgccaaggagtgctg agcgcggggagcccaaaccacagccttggggaacccaggaggggccgggcgggtgcagga cccgcatgagggattccgagagcacgatgtttaaatgttgggggaaaaaatggatcaaag cgatgtgttcccagggagcccctgttttctcgtttcttttcgtacgcccagctttgcagt gccagaaagctgttatctcagcactgctaaggaaaccccagcgcggcctcggagggatta ggcattctgctgaaccagggatatgcggtgggcctgaaaaagccatttcgggaggaatct ggtctcggctggggtccggccggcaccgtgaacaccacgtgaacaccacgtcggaaatct gctgggcagggagatttcctcaaggcctcccagggaggagagccacttcctgggctgcaa agagggccccaacctggcttctgaattcagagggggctgtgtgtgcacgtgtgtacgtac gtgcatgtgcacgtctgtgtacccatgtctgtggaagtggcacacgtgtgcatgtgtgca ctcagtgtacgtgtgcgtgcctgtgggtagctccgtgcgcacatgcgtacacatatgcct ggtgtgtgtgtgcacacgtgtgtctgacgtgtgtgtgctttgcgtgttcttgcctgggaa ggagttcccggtttgggtggctctcctgtttcttgccgaagccagctgctgctagaaact ggccccctgctctctgcagtaagagaccctgccctggcccaggtgagggcctggcttttg tctccaggatcacagcttttgtcaaactccacacaaagagagaggcactcaaaagcacaa cacaaacacggctagtccgcgcctttggtggtgaacataccagggaattgtcctgcaatg cctgtggttaaaaaaccccgtgagctgcggccccggaggcctaggggatgaggtcatcgc tgcggcttcctcccctctgctgaggctccctccgccccgcccggtggcccacccctcccg ccagctgcagcctccactgcctgggtttctgctgcgccttcagcacacagcgaggcctca gtttccctcccagctcagagcaaggcctcagtttccctcccagcagcaaagtgagaccct gtagggagtctctgggcctccgtgacggccctgggcggccctctctgtggaacaaggtcg ggtcctgtcctcagcgccctggcctccacttgctcagggcgcccgcaggcccgcaggtcc tcacctggtgtttttcacctgggacccgcagctgctctgatgaggagcctgctctgcgca cggcctggggcggtttcccgcagcaccatttctgccgctgccgggaaaagacgcagagca aaggcacctttgcccaggttggggaaccagtgggtgactcaggattttcctccacccacc ccgaggtctgactccctctgcagctgccgcccgctgagggttgtcttcctccctgcctgc ctcgccctgcctggtgagctcctatgggtctgacgaagccccgtcctgcacacttgcttc gggggcaactccccactagactggttgcccagggacgggggtacccactccctcctcagg gctgcagggtgctctgggaagagttgtgtccctctgtgggcctgtgggctcacttgcctt tttgtccctgtcacttgggagctacccagctaagaccggtccccaccacccaggccactc tgaggcttcagaacgcggtcgtcttctccctggcagggttgtttccgcctgcatttgttc agccagcaaatctttattaagcacctactgtgtgccaggatctatgtgcaagcagcccat gggctcatgaaaggcagagctggactgagattccgcacccctgggatcctcactgcaggg ccctgtgtatgtgcgggctggagccctggttcctgtctgcactgagcccctggagctagg cccagaggacttgacagatgtgggccctctgtctgcacagcccccagggcacggggtggc ctgcagctctgcccagccagacttcaggtcactgccccatgagcctgtaggagaaacttc tcactcatctgtcctgagacctgcctggagcccaccccagggagacagaggctctcggag cctgttcagggtgaaaggacgctagacactggcctgcaggctttgggcgggtaagggtag cgaaggtgaaagtggaccaggggcaggtggagcagggtaggcccaagtgtctgtctgggg cagctggggacacccaggtgggctgaacttcagctgccacccagcttcagctgccaaggc tcctcccgggtcagggtcgacccctgatgtcccggcaagagttccaagaacaaaggctca gaatccaccaagcggaacttgaagccactgccctgtggggacaggagaagtgacttggcc ggctctccggagcccagtgttgatcactgaggaccaccggatacaagtccctcctgcctt cccctttagtgacactgaagCTGTTGAAATCCCCATCCACPrimer
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