Mouse element [ mm2117 ]Position: chr9:63,546,991-63,551,986 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Smad3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence AGCCCTGGGAAACTACATGCaaatttgctttctctgggcaggggatggaggcaggtaaac atgagctaggtatctatctggctttggagtcagcaggtcctggctcagctcccagctctc tctatgtggccttgggagagctgctgagcattttgaactcacttccccatcagtcaaatg cacaaggcaggcaggcaggcaggcagtcagccagccactagccgggctactgggaggaca gaatggaatcaccgaagttgtgtgtccagctcaggcctgggacacacacacacacacagg tgcatttccttcctcctttcctgaagaaaacaacaagctccgaatcccacaggactcccc gctggctgtgtaactcaagtgagccacagcctctgggaacgcgcctgctaccctccaaag agctggggacactagcaggtagagtagatgctacaaatatactgcacactacagtgagcc ccctagccacacatggctactgagcctcagtttctctggctggagccaaaatacttattt acttatttgcttgtttattgatttgttatttttaatcttaggtgcattggtgttttgcct gcaagcctgcctgggtgaggatgttggatcccttggaactggagctaaagagctaccatg tgggtgctgagaattgaactcaggacctctggaagaacagccagtactctttactactga gccatctctccagcctaaaggcaaaatacttaaaataactctataactcacactatattt gtctatcagacatcccggacttagaattctcaaaaaccatttcaacaataacaacaacaa caacaacaaaaaccttgcatataattcttttcacagatcaagagcaacctcttcttgttc catctagcccaagtgcctgtcaagatactcagaagaatctaagaaaatccaaacattgtg gaaatggcaagtcgtcaccatacacctgggagaaagtcaatggactttgtccatactagc ttcctcgaccctcacaaaccccaccagggagtcctatcccttcactaatacagactagaa aagcgggggagggggagctggagagagcacagcagttaaaggcacttgttctcacagagg agccaggtttggttcccagctcacagtcacccgtaactcccgagtctgagatctaaaacc catttctgcccttggcaggcatctggcacacacattcaggtaaagtactcatacgcataa aacctcttaaggaaaaaaagaaaagagaggagaagagaagaggagaggagaggagaggag aggagaggagaggagaggagaggagaggagaggagaggagaggagaggagaggagaggag aggaaaaagaaaagaaaagaaaagaaaagaaaagaaaagaaaagaaaagaaaagaaaaga aaagaaaagaaaagaggggctagagagatggctcggttagggaagtgtctgtcacacaag catgaaggcctgtgttaggatccccatcacccacatgagggccaggggcagcactggacc acagaggtgggtccctggagctcgcctgcttgctggcccagctggattaatgtaacatgc tctaggtacagtgagaaaccctgtctgaaacaatatggtgggacacacacacacacacac acacacacacacacagagtcaaggctcagaaaggttaatgacttggccagtgctgtagaa tgggtcaggagcagaagactgggatccaaacccagttctgattctcatgcgcatgccctt cttcacagtgcatgtaatgaagcaggcagcccatacagcattctctacattacacatagc ctacctagtaagacttccaatacccaccccacccgcacaaaaggtttcccagcaggtcaa gaataaagaccataaaaaaagagtccatgtagaaccatatccacaaatagctgggcctcg actagctcagacttagtcatatttcctaagtgatgggaaaggcacattttattttattgc ctttgaacttctctgccagggtactgggcagaacccaaaggcagaaaacctgtgaggcat agggtaggtagatatacaaccagccaatgagccaccagtccagtcaaccccattgtccag gcagggacaatgatacaacttctgagacttgagaccaaggacatacacaggacaataaga accctgcaaaaactgtactgtcacatggccggctgagactgagtggcctactgccctctg caggccttagtgcccagaagagcagagacaggtggccagtcacctgttcaaggagaggac ctgcctgaggaggacccaggactgaggactaaagacaagctcttttctttttaaaggtgg tgtccggcaggcggagagggaggaggaggaaaaacaagacgccagtagacgtttcccatt caacctcagacagggtaaatatttgctcgtccactgtgagcttggctctgagagaatcct cagcttggccgcctgggttcacactggcatctggtcttgatttgaggcccaaggcagcca gtgcttagcccatggcaccgagaccagtctcctgctttgtctgagagctaaatggatccc actggacaacagtgagagaacacccaccagagaggccctgttgctcacacacaagcacca ggccatgctggcaatgaggcggcccatgtgggctgtgctcatctcttccccagggagctg catgggaggcgcatggcctccaactccaccacgaagagcctaggacagagcagacacaca cacacacacacacacacacacacacacacacacacacacacacgccagtgccacaccacc actggagccagccacactatccacaagctgagggagatctatttcctgagggcctactgt gtgccagaaattgcttaaagttttgagtgacagccatggataagactggaaaaaagtcat ctttagggagtggccatttaacacagaatgacaaattatagtattttcatggctgacaca ggttgaccgtctggagcccagaagtctggggtccggacacagtgtcttggatacacccca ccactcgagaggaaatgaggggcaggaatcacaattcatgctccaatctcctgacgtgta aagaaggcttcaagaacatctggcttgacaccccttgacaagatggaactaagctcccaa aaggcctcgaaggttacctgtactcactctaaggaagtgacataaactctgacaccagag caccagcacgcacagagctaaaatgaggaaagacgaaacccaaacagcagcccagggact cgcagctgtccatcaaagactgtttcccagggacgaacgcccccacacgctggccctgcc aagacagataactcttagtcaagcaagttgcttaactgggcctgtgtgtgggttcccagg ccgtggtcccaccctgtgggaacccagcatgtggggctcccacttaagtgagcggctgga atcaatcgcccctcggccgcagctcttgcccacaagttgctcttctggtcaacgcgcttg agctgagcggtgacctaagccagcccaaatcggctcggcagcagcctctgtcatcctgct tcagtgagcacaaggctcacatttcagaccatttgctcccttgggcatggctcagaggac tcagctgtctttaaaaaaatgcttttcatatttcaaatcaaaaataaataccacgcactc tggaagcaagccatcacagatgtctgctgaactataaaagtgaaaggggccgagaaggct gagaaagtggtgggtcatgttcccctgcgctgactgcctccgtctacaacgggatggaag agcaggactgaagctcaggtcacaggctcgtgtctggtgtgaccaatgacaataaagggg ggggggcacatattcgtactagttgaggaaagcatgtgccctttgggcttatccagaaat gccacctcacagaaggttcctgcaaacacagtgggaaaaagagtaggtggctggcacagg agctcgtgggctcaggtgcatctaacagaagggggggaggtctagcatcaggggctgggt cagcaagttgcacagaggtgtgcccagttatccatccacagacgttcaccaagtttactt ctggtgactcctgacttcctttattctgcccttctgccctcttggcttccctagtaggtg cccacaagagcatggtgacatgcattctgggaaatgcttggctaggtgacttacactcag ttactgccacactgcattctcttctatgtcagggcgggggtttgggggtgctgtgcacat atatggtatagcctctgactcctgaggctacaatgagcaaatcaaccagagattacatca tacacaggagaaaatgacaaagccaagagatgcataaacacagggtatcggactccgtca gcacagtgtggcatcgtctgtgggcaccgtttggctctccagtgcacctcagtgaaaaag cttgtggtctgatttgtaatcatggcttgattggtgtttcagatacttgctggtctgtgg aagccagcatccctgttcccaaacctcacaaatgaggctccaaatcattaggaagtatgt tctaaaataataaaaatataataaatatgtaaaccggtagaacactattattctctgggt aggattgtatgtgctctgtttttattctacaggcatgagactcaggtgtttccacatcta tatcacctgacatgtgtgtgtgtgctcgagagtgtgctatgacagccataacataaccaa tgatgggacttcttttcagctccattataatctttgaggacagctgttatgcatacagct tctccttagccaaacatcatgatgtagcacacgagggtaaattcacagtacctgtgCTTT GCAGTGGTCGACTCCTPrimer
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