Mouse element [ mm2066 ]Position: chr13:14,806,534-14,811,790 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: AW209491 - Gli3 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TGGAGACATCAGGGATACAAtggatatacctcaatgtaacataggcagtttgctgtcagc ccatagccaatgtcaacctaaatggagagaaattaaaagaatttccactaaaatcaggaa caacacaaggatgaacatcttcatacctgttcttgaactcttattgccagagcaataaga aaactgaaggaagccaagagaatataactagaaaaggaagaaatgaaagtatctttattt gtgtatacttttataattaagagacctaaagactctaccaggaaacatctacagatgata aacactttcagaaaaagtagcagtacacaaaattaacagacaaaattcagtcaccttgct ataaacaaatgacaaacatactgaggaagaaataaaggaaacagtacctttaacaatagc ctcagatatagattgtgataatgcaaccaagtaagtgataaagtactataaaatttttat gggattaaagaaggctcttgaatactgtagaatcagtaggattaatattgtgaaaacagt cattctaccaaaagcaatccacagattcaatgtaattcccatcaaaattctaatacaatt cttcacagaaataaaaaaaaagtcttcatttttatacaaaatcacaaaatacccagtata gctaaacaattataaataataaaagaacttattgcagtatcaccattgtagatttcaagt tgtagaatgataatagcataaaataataatatcacaaataaaataaaaatatcataaata aagacacacacacacacatacatacatttgaatgaaaatttcccatcttgactgataatc tacctccaagagtcaaagatgatctaacaaaagttccaacactaagggactcccaatgtt ttacatattataactatactgttgaattgtcccccagaggtagaaagtaagtacttattg ctgaagataccattcatttcagacatggggctcagaggctcctaagctggaattgacctg aatgtttcctctatgtagactaactctcatggtaccagaagattccatgtaaccttccaa aggatggaagcagctaatagccctatcttatctatgatggctatgaactacagcaatgac caaagtggtatgataactgtacagctgtgctactggcacacatacctttgtgataacaat agctttctaactggggcataacctctgctaaacaagagggaaatcaagcctggtacctga aacctagctaactacgcagggctagtaaagccacagaccttggagtaagacctacaactt agtctttcctaaaccagagtaatccacgactatattctggatgtctgttcttataacatg taattaattcctcaccctgcatcaaagaaacttccatttggaatagaaggagatcattat aaaagacaagaactaactaaaatgcagaagtgttgagcccagacccaactgatacatctt taatacatctaccactcttaaggctcagggatcattgagaaaaagggaaaaggactgtaa gagacagtagatcagagttttgtgggattttgtctcctaggaatgtcaaatacttccctc aataaggtctctcccacatgtccacctaaacatgagccaatcaagacaacaatggacatg ctaaagttgacaggaaacaagcccacaagtcctcaactgtacacacacacacacacacac aaaaatagatacaactgagaaatatcaagtgtgggaaaaatgcacttcccagagacgatc acaatgattggctatccaataccaatggtcagtcctgaaagcacacatataagtactatt attcagactaagtacataaatgtgtgtgtgtgtatgtatgtgtgcatatatatgtatgta taatgataattaataaaaagaccataaagttgaatgtgaacaaggataggtattggaggg aagaaagagaaggggaaattttatatttttattataatctccccccaaaataataattaa aaaataaaccttcctaaatattggcatctgagccctctgaagaactttttagaagttggt ttttgccccaaaagaactagagcatttttcttcatggctatgtaaaccacagggcctctg aaacatgagtgaaaaagcattgaatagccacctcaaatatttcagaagaaaatatcttct gttgaaactatatagatactcttcataaaataaacttagtatataattactactccttct tacagtaaataataataataataaatataataataaatctaaaaatgaagagaccagtac cactgaggtcacatctaaaaaatgtttcttcaggttctgcacacagaaattgtgctccac atgctggcagctaaatttggtaatatgtaagattttcccaggtagtactagtaatgaagt catggagagcagtagtagaggcttagcactatgagaggccaggagggtcattgtaaatgt gcagccttagttaaagtagaagctaaaggattgaaggggacatggagagaatttgaggct tagcaccatgtggcagtgtcagagtccccaaagaaagcccaggcggccattggtgaaggg gcagcccagtttcagtggagaacccaaccttcagaagatgccagttccctgggatgactg acaaggaaagcagcagccttggagtgaagccaactggagcttagaagacaaactgtgtgt tgtggatgtcagtgccagagaagtgccacggccaagttcctttggagtctagaaaattgt gactgggtcatgtacatcaagcattaaactttttatgctgttgaaattcagttttgcttt gctttgattgtgactctgacctggatgtcctctcttgaagtaggaaagttttaacttgtt tttctgattttgcagtaaccagttatgtgatatttgatagtttagaaaggccttggatac tatagagagacttcagcatttttcagagacattagagtatcagaaataccttaactgttt tagagagattaaacttttaaataaaagactgaatttttaaagcattcaaacttttaaaga agttttaaagaagttttaaaagtttgaatattttatatttgtgatattaatattcatatg aaacattgaggatcgactaggaaggaaaggttatggtttacagtgatgcatttttatgtc aagttgacaagaggtcaattgtgctgcttaattttatttcaacttgacacaagtgagagt tattttggaaggcagactctcagttgagaaaatgatcctaccagaatcaccatgggcagt gccaccttgggctggtgggtcctgattatacaaagaagcaagccgtccaagtcctgcaga acaagctagtaagaagcacccctccatggcatctgcatcagctcctgcctccaggttact gccctgcttgagttcctgtcctgatttcctttgttgatggactatggtgtgcaagtataa ttctaataaaccccgttctcccaagttgcttttggtcattgtattttatcagcaacagaa accctaaataagtcactgtgtattcactccttccattccgtcatgcagtgaacacagctt tcaaattctgtaatgctgctctcctaatgaatgatgtaatggaagcagggcatgtagact tctcttttggctgggtaaattctgctttttctcccactcactgcaggtgtttcaactcat gcaagaaaaatcagattttccgtgcttagaccaaccttctcaaatttctcaccagcaggg cacaaggtcctaattacttcatgggtaccagctaattaattaaaaaattatttttcgcag cctttaaagtgagttcagccagacactatttgggctagaaatgtcattttcatgtctctg ctctgttgacaaaatgataggatgataatttagctgagaagaatgaaatgaatgggctgg tcatttcctctgtgattcaaagtaatcgcaggcagaaccttgcagagattccaaatttgc tctaggtcactctgatttgcactccatactcttgccataaggtttctttctttctttttt ttttatccaagtactgttttcttttcttttctctttctcaaagattcaatttgaaatgat cagatgaatgtagtgattctaagaaaactaaatgagtttttcctattgtgatatctttat tttaaaatgcacttttaaggcaatactactctatggccttacaatcagctcctgattcta ggttcaagttcctgccttggctttcctcaagtatttaaaaagataccttcatacttgagc ttcatttaaaaacaaaattaatttctagatagttcttcataaatttttcatacttccatt ttattattttgtataaaagaccataaggacacactacttatcatcatacatataggaggc agctggaaaaatatttatacagtagctctaagatttaatagagagcagttgattttttca tgtttttgttaagcatgagttagtcactatgtgtggtgttgcatgactgtaatctatgat gtgatgtggtggaaactgaagaatgacaagatcaagaccagcctgaactatacagcataa ccctgtctcagaaaagggaagaaagaaatgatcatttgtgtttgtgcttcataattgtct atatcagtctgttagttttgaaggtgtaattagtgctcatctggtttgatggtcttatct tagagaagaagaaatcaatataaagaagaatgaaactgacatctggaattggctcaatag tgattaaatccagtatggccactcaacaactcaaataagaaaatagacatcagttttctt gctgggcacagacaggtatgaaaacctcttgtttgcatggcctgatggttcacttagcaa taacctgtgggagaggagttcagtgcataaaagaacttgaggccttcaacaattcaagtg atcttcaggttttctgtagaggtctgactcttcctttaataataagatacttaaaatgat atgcaaagtcctctggttatgtatttaggactagtatagctgaatcataggatagttcta ttttttagctttttgaaagattcacatattgatttccaaagtacagttcattcttaccaa ggctccctctttccccaTATTCACACCAGCATTTGCTPrimer
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