Mouse element [ mm2061 ]Position: chr13:14,850,845-14,856,161 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: AW209491 - Gli3 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence AGAATTGGAGACCCAGAAATGAacccacacacctatgttcacttgatctttgacaaagga gctgaaacaatccagcattttcaacaaatggtgctggttcaactggtcattagcatgtag aaaaatgcaaatcaaattgttcttacctccttgtacaaagttcaagtccaagttgatcaa ggaactccacataaaaccagatacactgaatctaatagaagagaaagtggggaaaagcct caaagatatgggcacaggggaaaattcctgaacacaacaccagtagcttatacttcaaaa tcaagaattgacagatgggacttcataaaattgcaaagcttctgtaaggcaaaggacact gtcaataggacaaaacagcaacctgcagattgggaaaagatctttaccaaccctacatct aatagagggctaatatccaatctatacaaagaagttagactccagaaaaacaaataatcc tcttaaaaatggggtacagagctaaacaaagagttcacaaatgaggagactcaaatggct aagaagcacctaaagaaatgctcaacacatttagtcatcagggaaatgaaaatcaaaaca accctgagattcttcctcacaccagtcagaatggccaagtctaaaactcaggtgacagca gatgctggtgaggatgtagagaaagaggaacactcatccattgcttgtgggattctaatc tcctacaaacattctggaaataagtctggtggttcctcagaaaattggacatagtaatac ctgagaaaccagctataccactcctgggcatatacccagaagatactccaacatatacta aggacacatgctccactgtgttcatagcagccttatttataataggcagatgctggaaat aacccagatgtccctcaacagaggaatggatacagaaaatgtagttcatttatataatgg agtactactcagatattaaaaaacaaatttatgaaattcttaggcaaatggatggcacta gaagatatcatcttgagtaacataacccaatcacaaaagaacacacaggtatgtacttac tgataagtggatattagcccagaagcctggaatacccaaggtacaattcacagaccacat caagctcaagaagaaggaagaccaaagtgtggatactttggtccttcttagaaggggtaa caatatgcccatgggaggagatacaaagacaaattgtggagcagatactgaaggaaatgc catccagagacttccccagctggggatccatcccatgtacagtgaccaaacccagacact tttgtgaatgccaacaagtgcttgctgacaggagcctgatatagctgtctcctgagaggt tttgccagtgcctgacaaatacagaggtggatgctctcagctaaccattagactgaacac atggtccccattggaggtgctagagaaaggacccatgagctgaaggggtttgaagtccca taggagaaataacaatatgaaccaaccagtacccccagagttcccaggcactaaaccacc aaccaaagagtacacatggagggattcatggcttcagttgctatatatatcagaggatag ccttgtcagtcataaatgggaggagaggcccttggtcctgtgaaggcttgaagtcccgtt gtagggaattggcaggaccagtaagtgggagtggtttggttggtgagcaggggcaagggg tggggataggtggttttcggaggggaaaacaggaaaggggctaacattttaaatgtaaat aaataagacctctaacaaaagtttggggttttttttttaatccttaaaaacagtaacttg taaaatccaaatgtttatccagaaatgtgacattatagtacttggaagacaaatttatac ttcaaaattattaaagtaaatgtaattttcagactatctatatccacaactggttggtta agtttaaaacaaaccaagtagataagtaacatatccattttacaacatgtgtttaacatt gagtagaaacttggagatttagattcactttaaaattgtccacccgttcatatctcagat tcaaaaatccagtagatacagacaggataacaacttactaaattttgcttggaaccatta ttgactggtatatcatttactaggcttgccacatctacttatagtgatagaatttaattc taattacaaatattcactgattcaattacatataaattgtgctgctcaggccactgttga gaaatgaaagaaagctggagagctagttcaatgagtaaagttcttgccaggcaaacaatg agacctagaatatggattcccggagcccacacaaaactaggcacagtggcacacaactat tgttaggggcctggggaaagagttgacagagatgccagtctagccaaatgggtgagatca ggttcagtgagagaccttgtcttaagaaaggtggagagcaattaaggaagacattactaa ccttttccctcaatacgtacacactcacattaatacatatttactcatatgtgcatgcac tcgcacagaatcacactcataaggacacacgtatccctctcagctatatgtagagaaaga gagacgtaaagaaatgtaagcattgttctaattcagtaatctaacataaaacaagtcaca ttcatattgtggagataaaaccattgcaacatgctgttcagattccagaggcaatacaca gaaagcttaggaccttttcaatattgtttgatgcactttgttaaaatttaagttatggga atatgacttacatctctgcctgcattgtgaatatatttgtatacgtgcaattctttgaac ccagtagtgagcataaattgccattcatgtaggacactgcttattatctaattagcttgg aaaacaagataaattttgttttagaaactttttctcgggatctcagaacttgctctggaa ataaactcaaagcagatgctgcagttttcttgaactacaattagagtaagatcaaatgta tggcaggttttgaatgccttgtttcagccttggtccatagtttatgttgctctgtcactg taaatctttccacttgcttgtgggcctttcctgtgttagatctagtcttcaacatgggta cacagcttctgcagaaaagcagtcaattttggaagtttccatgaacttgtgggtcttatc atgtgtcctatcttgtctacaccatgggtccagaaatcttctcttttataaagatataga agtatatactaaattatattaaatatattaacagattatataattatatataatatatac ataatatatacagatattaagtttaaaattttaatttatgcaaatagaagacctaatgct gtgaaattagattcaagatgcattccacccaaatgaagtagcttaaatggactcaaataa tacttatctgtgtgttatgatattgagttgaacaatgagacaatatggtttagatatatg tgctctacagggatactttgcattatttatgtctatatcacttttatcaagtaaatgaac ctgcagcattgattaagaacaaattaagttacgaggtcataatttgattatatcactaag aggatagatttattagagtagcaatactgagagtgtaaatagaatcagtccatagaaaat gcattattgtaatacttttccagctacagagattgaatacctcttggcttgagagaaatg gcgggagtctgaagaacaagtttgagaacgattgtaatatatttaaaatagctacatcag tggtgattttagttgtgatttagcacatacaacacaagtgtagaaacatcccgtgactaa cttattgagctgcaaggaaatctagactatgttgtattagcttatgactgacaaaataaa gttggggcctcagtcttaacagaaattttccaaatatcattacacattaatctaaagaac tccagaagcacagttttaaaatactgctctaagtgagattggtctgaaattttctttcat tgttgggtctttgtgtgttttaggtatcagagtccacctccagtacatagatagggactc aagtggtagaacaggattactaacctacagtcaaaattcctgacccagaattgttcctgt ctaaaagaactacagggccaaaatggagaagagactgaaggaaaggaggttcaatgactg gcacaacctgggattcatctaatggaggggcaccaagacctgacactattactaatgcta tagtgcgcttacagaggggcgcctgccatggcagtcctctgagaggccctaccagcagct gactgagccagacctagatacttacactcaaccattggactgaagtcagggacccaatgg ctgaattagggggaggattgaagaagctgaaagggagagcgaccccacaggaagaccatc actctcaactaatccagactccatggagctcccagagaatgagccaccaaccagaagcat acacgagctgatccaaggcccctggcacatatgtagcagaggtctacctggtctggcttc agtgggagaagatgtacttaatcctcgagaaccttgatgctccagggaaggaagcagcct ggtgggcagagggggagagcaccctcttggaggcaaggggaaggaggaatgggatgaaga actgagggagagggtgttggtggggcaatgaccaaaatgtaaatatactgctctagaatg actttgaaaccatattcgttgggttgttgttctcttttttccatttcccctctcatggga ctactgtattgacaaggctagttcttctcctaaggaaatgtattccttttcatttgtttg tttggtgtttttccttttatagaaaatagaatttggatcttggtccgagacccgccgaaa ttaggaaattagtctgaacaggtgagagggtgcgccagagaacctgacagcttctggaac aggcagaagcacagaggcgctgaggcagcaccctgtgtgggccggggacagccggccacc ttccggaccggaggacaggtgcccacccggcaggggaggcggcctaagccacagcagcag cggtcgccatcttggtcccgggactccaaggaacttaggaatttagtctgcttaggtgag agtctgtaccacctgggaactgccaaagcaacacagtgtctgagaaaggtcctgttttgg gccttcttcttcggcCAGGAGGAGGTCCAAATACAAGPrimer
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