Mouse element [ mm2033 ]Position: chr13:15,858,225-15,863,372 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3 - 4933412O06Rik Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GGGTTTCTATTCCTGCACAGacatcatggccaagaagcaatatggggagggaagggttta ttcagtttacacttccacattgctgttcatcaccagaggaagtcaggactggaactcaag caggtcaggaagcaggagctgatgcagagtccatgaagggatgttacttactggcttgct tcccctggtttgttcatcttgctttcttatagaacctagaattaccagctcagggatggc accacccacaataggccctctgacccttgatcactaattgagaaaattcctcacagctgg atctcatggaggcatttcctcaaggaaggctcctttctatatgataactccagtttgtgt caagttgacacaaaaccagccaatacactggccaaagctatcttatgtctaggactcaga aaaatcaaatcaaatacaggcagcacagcataggaggaggaagacagacagacacctgtg gactaccatgccacccacagatctgccctctagcacaggcccatagcactcacttgctgc cacatcatacagcacaaataaaggacatccccacgggcctatcagccacatgactagccg gcagtgagagcccccacaatgggcaagtatgtagtagtcagatcatagagagagacaaca ggagcagcttgtgtgttataaagagagatgaaactggtgactcaagcctgaagagtagcc tgagtggtgaaccctgccacctggggcctatggtgaggtcctagcctgagctactgttga ggaccatgtctgagtctgtggctatgtagccacagactgggattgttgtcatggctccat ggctcatattactacaagagaacatggggatgtctctggttgggattgctgccagggacc acattgaatgtccaaaggctgtgtgtgcataactggccccatccctcactggataaggca ctctggagagctgaccccatctcttactgcctgaaaaacttgagaaaggaagccctgcac ctgtcccaggcagcaaagtggaactggttttggtgtttggggtgtgggtgagtcagccct aagagtgtgagcatgggagagctgacccgccactcatttaccatggggtggcatgggtgc agatatgatgaccccccactgctcaccacctttggcacttgggacagctgcccacagagt catgagatcagaagctgtccctcaccagctgtagtatcctagtagtgaggaattcacgtg aaccagcggtgagagcaagagcatgggagagcttgtcccaccactcctctgttgtggggt gatgtgggtataaaagtgatgcctccccccaccccccacctcttgccatgttgggtagtt gggagagatggctctggaccatgagagcaggtggtttggccttgtccctcactggctaca gcacttgggagagtgatccttgcaccttacctgggcagcacagtagagttggccctggtg gattgagagcaggtgagttagccctgaaggtgagagtgcaggagagctggttctgccact cctctgccatgaggtggcttggtgggtgacaccctctgcccccttagccctcgacacatg agtcagttgggagagctgaccccagagaaatgagagtgggagagctagtcctgccccctt attggctgtagcactttgagagagcatgttctttacattgcctgggccatacagtggagt tggtcctgatgatgaaggcagggttgagcggtcctgagggtgtgggagcagcagagctgc cccagctcctcatagagtgcaatacttggggggttggaccctgaacctctactggacagc acagtggacttggctctggaggtgtgggtgctggagagctggccctgaagaaggcatgta agcagaagagctgaccctgcctcctgttgatggcagcactgggcaggctaactggggcaa tgctggagaacttgctctggtcgtgtggataaaggagagatggtgggctgaccagctcag ctaccacccaggcctagatccagggctctcaattagcccatctcaaaatatttatcatct tcaaatgattgggatctgtgaaaaggacagtcctgctgttccaaggttgcatggtctcca tgatacagggcaacaagaagattagcattaagaggcctgaagaagatccagtgttgtcac agaaactaaagaacttggaccagactcattgcaatgaacatttgcaagtgaagatgtgtg gacagagggatatactgtgggatatacagaaagacactctgcaacataaaacagcttcca tgatgaggtattttctatgctttgagtttgttgttgttgttatagttgtttgtttgtttt attttggcagaaaggttgcaaggatgaagggctgatatgagaggacaaggagaaggagat gagtgggactagagtccatgatgtgaaacaaagaatcaataaacaatttaaaaagaaaag aaaggaaaggaaaaggaaagaaagaagctttgggtgcaaagaaatgataaaagaaagact ggtatataaaacatagctcctccaaatcaagcatttattgataaaactccttgtaggcat aatttttttgcaatatacttttaataagggctcaacttctcctctagtccatcacccaac agaggtagtggaaaagttgttaggctctgttcagcagtagttctctgggggtgagactga tcttctttggcagctgttcagttccgtagcaaatatcaaatacaaattaggaactgcaaa ccagtccccccaggcaatcagacaccaggcatgaaccagcaactacacagtccagttctt tcagccagcagacacccggcagctgtcgtttaatcctgaagaaaccaccatgttcccaac cctaaccagcctgaggtaaggccacagaagctgcaagctgcggcagaaaccttgagagca gttcttgggtgagtttctctcaatggtgatgttattacaagttgagctcaacaatgctat ggaaggagaaccaatacatgcatgttgttaatgaagactaatgaggcggaggaaactaca ccaagattcagtgctcatctcccactgtctgtgggttcatatttatattccttcatcaag agtcagtcctttcatgtatttgctaactccacacatcctttcacctgtgtctgcttcaag aaaacaatctttcacaagtttgcattagtaagacatcttttcacctgtgtgcctcagcaa aacatcatttgacataacattccaaagaactagaagtttccacttcaactcattaggtta ctgtatgtttttaatgctgtcttagtttggttttcactgccgggataaaaccacaatcaa cagcaatttatagggaggaaaagatttatttgaattataaattacagctcaccattgaag gaatctgaggcaggaacctggaggtgagaattaaagcagagaccataggtaaaggttgct cactagcttgttcagcgacctttgcttacagtttcatttgccataggttcatggtaccac tcacagtgggctgcatcttcctacaaccaattaccaattcagaaaattccatacagacat gcctgggccagtgtgatggaggtgttttttcaggtgtgttttttcccctagcagtatcaa attgtcaactaaagctaaccaggacagttgctaaatgtttagttgtttattctactaaga gctgtaagtatgctaagttcagttatttcatgcatcaccctaagatgtagttactgtatt ccattttacagtcctgaaaagtgaagttaagagaattttataaccatttcaagatgacat agcaaataaatagtgaagtgttaaattcccacaaagaaggtaaaggaaagttgaaatcac tcatcttggtacctaacatagacttttcatttcttgaaaaccatgcctcttcccatggaa aaagaataagaatcgaggaagatgaaattggtaattgttgtttgaatattcatttaccta tttgttttatgtcaagtgttgtagaaattagaccccagtacctggagctctctattcaaa atacttattggagagactgctgagatgatgaggtaagcaggctggccagaagttgagcta cagaagtgtagtaactggactgggaatgagaatcattttaatagaggtgagggagtcagg tctttgtgtcccagaaagaccattcatgggttcaagttgtccgtagggaaagggcacagc cttgggagagacattttcttttggctgatgataatttacaaaagaggatcctctgtaagc cctaactctcctatgctcccattgcctgatagagtaagtactcccaaatggaagagagct ttggcagcacagtgcacagcacagcatccgattgctcttaccctgcactgttcaaacata ttcactccatagtggctttactgctacctaggaacatctgttccagaattctggttggtt tgctttatttgtgaaccaagccactgtacaaacttattcattccatctagtggatttacc actgcctaggaatagctgttccaaaattttagttggtttaatttattgcagaaacttata agataaaagccagtaggaggaagccacagcaggactggggttacaatgtattcttcctca attcctctcttaatatctattctagatggcactcttaatggctagcatctctgatcgtgg ctttctgttgggtaatgaagacctctttataccttacagtcagtgccccttatgccatga cattctcaactcatggctataacatgtcttatctaattgaactgcagagtatgaaactgc agagcattatcttagtagatcacttagccacaattatatcttgagttgattggacaacca tagcctgggttcctcatgatgaataaatgtcaatgacttttgcaagtgaagttacatctt gactcctgaactcttgatacaatgagcctgagctcctcaggtttctctagccacaaaatc tagtggaaccattacaatgatgattcctCTGTTGGGATTAGGACTTGGPrimer
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