Mouse element [ mm2009 ]

Position: chr8:47,305,342-47,306,646 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: Slc25a4 - Helt

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.

Fasta sequence

TGCAGCCCTGTGAGAGAAGccacctagatctcacttgactgattttccaagtacattttg
aactggttcctccactgtttagtgtttcatgtaaaacattatattcctgttattataaac
aaatgcttaacacacaatatcagaggttttttttttaaccaaaaaaaaaaaaaaaaccaa
cgctttaatcttgtgaagtttcttgctatcttcagtatggtctcttgtttgttcatatac
tcaagacatactgtatcagtgaagcagaaaaaaaataaaacaccaagaaatcaaagaatg
tactctattattagcatgataattttttaaatcaagaaagaagtcatatatagacaacaa
atatgtcataggagatcaaaggaaaaacaaaacttcctgcccacctgtccatccaaagat
tatataaggatttccagacatggataggacagagagagacacacacacacagagagagag
agaaagagaggatgtgagtgtgatggggtcaccgacaaagaagagctctgtggtctgctc
caggcaaaggaggtagcaagacaccagggagaaaggactctctggtttggttgtagtgcc
aggttagatgcagcccagggagagcacccaggaggaagaggagctcggaggagcaaattc
tgggcacctgccatgtagtctgctggtgtactgggccctgggtggccccgttaagtttca
cctgccccaatgtatttgtagaagcaacagagtaacactttgtatccaaggacaccagtt
acccagaagagccttgtgcccagaagtgagggaagtgtcagaagtgaccttgatggaggg
tgactgaaggcatgaggacctggcagcccagcctggtcagctagagttgcagaccatgtg
gctctttctctctctctgcactatagaaatccatgggatgtagatgtcacccactggaaa
gcaggagagaaaaggcgaggtggatacttggtagaatgagctctctaattgaaattaagt
tgcctttcgcagaaccaatattctttcctattggtagggcttttcttatttataaaaggc
aacagactgaagaattttaaaattctacactgttcctcattccatccatcacatttattt
aagaagataataaatgagagagagaatattaggagagtaggggtacttctgggcttcttt
ctctatgagtcttcagcctatacatgcaaagtacaactgcaagttgctgttttgatgtaa
caacaacacagtaccttgaacctaACAGGTGTTTAGCATATTCGC

Primer

(+)TGCAGCCCTGTGAGAGAAG

(+)GCGAATATGCTAAACACCTGT