Mouse element [ mm1978 ]Position: chr13:15,671,935-15,676,645 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GGAGCAAGCCAGTAAGCAGTATccctccatggcctcttcatcactcacgcctctaggttc ctgctggttgagttcctgtcctggcattcattcttcagcgatggactataatagctaaat aaaccctttcttccccaacttgattttgatcatggtgttttgccacagcaagagaaaccc taactaagattgtggtttttgtttgttttttaaaaaactaagtcatttttttttaatttt taaatttaatagggaatggatttttgaaagttagtgttaggaacttgttgtttaaaagat ttagtattaaattcattccttatatttgataggatcttctgacagcatgatcatcaaata cattcttgcatttgcagcgtttgataatgaactttaccatttacatggacatattattaa ccgtggtgttggccagctatggagacagcagccgacagccctgggctgtgtctcccagac catggaatcctcagtggcatgtggggctggaaagccccaacctgggtttcttttaaaatg gtatgtaaatgcacaccaaatccacatccatcacttggatctaaactgtactgtaaatta gcatgcatggctgttgaggtcacactctttaccagaatggctttaacgtaacttggcagc ttcagcataaaacatcttttgctctgttttccggcaagcacaggcaaaaagcaagtcaaa actgctttcagtaatgagacctgatctttttaaaagctagagtttcctgtgtctcctgta cccattttctaatttaccaggtaacacagtcaggactgcagtgccctaaggggaatttcc tcatttaaaatcctcatctggtggtcatttttcttaaaacttggttatggcagttagtat ctatgggactttttaattctttagacttactaaaaggctttggtacttggtactgcagta tgtaagactgcctgtcaaagatgggattataaagaaaggccagagccatataagggcaca cctaggtcatagagtggaaacattgcttaaaaaggcatccatggattcagaggcccttcc tgtagctgccctctctcccctttcccagtacccagtctctctctcttccatctggcttct ctgatcagtttactgactaacatcccagcaaagcatatcccctgacatggtaatatttaa atgccaagattaaaaattaagattgggttaaaaactgaggctccccacggagccgggact ctggagatactttgggcatttgcctcccatttatttaaagtgtgtgccaacactatgagt cctttgtaactgcaggggaaggagttgagatgcatagtatctgcaggccatgaatattgg taaaagtgaccattcataacggtgttgattgctaagaattactctggagtgcagagtaaa agagacttcaaaatgacttgccaggggttttgagaccacaaacttagcttctgctgcaga acgttccacgatggtgtttcagctacctgtacgtatgcacggttaatgtttatgtgtgaa tactcctggaaacaggcaggagtgaccagcaaagcctaattggtttcaaatggctttggc caagtctggtgacaaagctctaccctttacactgagaaagccatctacctggctctttaa aacttgctcatgtaacaggaatcaatggagaaaatttacagacaaattacaaaatgattc catcagttagaaaagataaacaatttagttttattttttatttttgtaaatctgaaactt ggacctaatgctatagatattagtgaagattttcagtttactcaaccagtttcaagggaa tggaattttttaacacaggtataggtgtttttcttctttttctgtttccttggagaattt taacccatgttcacattgactgcagcaaattaacccatgtaaactcatgttctgttcttt gaaattttgttgttttgattgaaccattatgtataatttaagttgaacatttcagtaaga gacctgtagctgggcaggtcagacaagattgcttttctccacattcaaatttggaagttt ttataaacatggtgtatttgggatctagttgtttcatcagagcaggctatgcaacattga atgcttcagttctggatatttggcttacctacattgctgtggttcctgctcctaggactg gttgcatggaaaaactaccttctgtgttcagtttgcagtagtttcttcctccggtctttg agaaagctcaagacagcctacagcaacctctatttcagacttatttttaatgtagccgtg ggaagatatgacagtcatttagcactcccctccaaccccaaattatataatgttctatga aaataaaatctttgagccagctccttcccactttcaggtgggaagaaatcacttcagatc acatctacctgtgatcatcattaaatgacttcacgcacagaagaaattttgtttcttctc tctgagggcagcatgaagttttcatccttagggaactgcaacaaatctcatacttcagtg agcttaccagaagctgaaaggtctacagtttgtatgcaaccttcatacatatcatatcac gtgcctattgggattcaaaaccaaaaccaaaccaacacattgatggtttatgctgtgttc cacttgcagaggtaaagacgtatggtttgttcctttatggagagctatatcttctgcttc tctgtccctgcctctgctattgcaagtacactgttatacttttaattgtgcttctcctta ttgtgcatgtacactcacacacacacacacacacacacacacacacacacgcacgcacac acgcacgcaggcaggcacacacgcacatgcacacatggtgaatttctgaggtggcttttc tgggcaacacatgacacactgggagtctccaccttcattgcagagtatgggcagacatgt tctcaagcctttttactctcatagccagtcaacctttcagacacttggctaacatgacgc cacatttgcattgctcaccagggatgcaggccgcacactgcctatcccatccttccttca tccctgttatttgttactggttacctcttctttcatggtccatgtagaatgtgtgctttc ttgccttcttatgaattgggggtgactttattgcttcttttacagatccgaatatttatt gcctttagactttttagtcaatgaattttgctcatcgttattattgttagaaagaatgtt agaaaattaaaattggtggtattattttctgtagccatgtgaccatataaatctaaataa agtctttaacaccatgaaggtgatctacttggagaaccaagacttgtttttttaagacag ttcatgatattggggtgtaggcgtaaattttgcctccttagtgtttgggcatcttcagca tcctggacattgacttctttcaggcttctatgatcatccttcattgattcttcatccatt tgccagccctcaagctcttttctgcatttctatggtgttatagactggaccaagacaact gtgtttctacagttgtgctgggtcttaccttttattctccattctcgtacagtctttgtg aatcattgggtgagtcaatgcttccttgctccaagaaatgtgtgaaacgatcgtctaaga aggagaaagtctgatatttcctaatgacttcggaggtccagtccatggatattttgcttc attgcccttaagatcatggcagggacacatgatagaactagttctgttgaccaggaagag tagacaaggaagagatggtgtcctacagttccccatcagtgaaagtcctcctttctagta ctgtaagctgggagtccgacctttaacgcatgggcatctgggagatcctcaagacctgta ttaaagctgtcacttgaatatcgttagtttctcagcctgcacacaatgtttgtgattgca ctgagcagtcacaataatctgaaaccattatcgtctttgtctgtgctcattttcctggac caaagagtggaacctgaggcaaaaccaaagaggactgaactggcccaggagcaatggtgt acaacttcccagatacttgatgctttagcactggtttctttctgctgtaactcgtgcacc tgaccttgactgttccacatgtgtctccctttggcatgcttccttgcctagcttccacct gtatgacattttgaggttatagcctccacttgcttcttgatgccctgttgatgcctacat tttggaatagcctcatttttcagttcttcagtcttactgtcttactcctgctttgttgat cttcttgaaccccccaccctccccatgcaaccttaaaagtgctggttctttgacaacctt cctagttcttggtctggcctacagcagccctgctactccaaacttatcagcactctgctt tctttgggtcgtccgacttttagtagatgtgtctttaatggggagtatcagttacccact gttgaatgaacaacccagacaatgatgtttaccttgtggtgctctcatctggtctggtgt ttgcttccaccaaccacatctggaacatgtgcatattctaacacaacatctagataggta tgtagcaagGTCTCCATGTTAGGTCTTTGCCPrimer
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