Mouse element [ mm1976 ]Position: chr13:15,622,676-15,627,639 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TCAACCCCATAGGAAGAACAATaatatcaaccaaccagagccccccaccccgagctccca gggactaaatcgtcaatcaaagagtacacatatagggacccatgtctccagctgcatatg tagcaaaggatggccttgtcaggcatcaatgggaagagaggcctttgttcctgtgaaggc tcaatgccaatgtttagggggaatttcagggtgggaggtaggagtgggtgggtgtgagaa caccctcacagagcagggtatagaggggttaggatagggggttgccaatgggaaaatcgg gaaatgggataacatttgaaatgtaaataaagaaaatatcctataaaaaaagaagaggta aaaggaattttcaagaatgtagaaattctgtattaaagccattcaagtttgagtcataga aaaggagaggcaaaacgaacattcctagccagagtgagggaagagcaagctagggacaag caaaggaaccattgactgtccagagagcggttcttaggaacattatagaaaacttattag tccaggtcaagccacacagtgttaaagaggagagacccagggttagggaagtggtgggtt tggtcgtattttgggtcatttatgaggtatgtgacagggaagactgcagcttagtttagt gcaagagatcgccatggcaggatcagtgtttttggtagaaagctgtaagttgaatatata ctttttacttgtctagaaacacagttctgtggtgtagttgtgaagattatattacagaag taagagactccttcccttctccctaactcctccttccgggatggaaattaaaagggaaat caaaaccagagcttagaaaggcggcttccctctggccacactgggatgggttgtgatagc tgccaacatgctgaacatgcccagaggtacatgctgtcgttttgtagggaagggctgtga catttgatgtctggactgtacctcacatcacacactaggccagaaggatacaatagaatt aacagaattgtcaggagtaataaaatcatgtggattatgtgttttagttgctgtttttgc cagctcttcttgagaactctggtagaactgcaccagacatggagattaagatagaagaat tcttatttgaatggcttggcaggaacactgtgtgaaattatgtattattattcccctgaa cttaaagtctttctgccaagaaatgcaactttccatttgtgtcaagaagatgtgaattaa ggattaaagaaaagtgaaagaaaacacagatgatctcaacttaaaccaaagatgcagtgt tgtggattttcagctgtaatactgtaccctggggtatggtaatggttaggctgagattgt taggctgagattgaccaattcttctataatttgtccttgagattttcctatattgtcttg tcagtgtctcctctaagatccttatttgttacctgagtcctctcccctttctgtccctcc acagttaccttactgtcttttttttttttttttttggttttcttattgtacaagtacatg agtcgcagcatagtggccattctgtttccatgccttagaatggtgattctcaacctctga gaggttaccccttaaagggggtaggagggtttaagcgaccctttcattgggatctcatat cagatattttcattatgattcataacagtggcaaacttacagttatgaagtagcaacaaa gatgattgtattgttggaggtcaccacaacacaagggagtgtattaaagagccacagcat tagagaagttgagaagctctgccttttttttttaaagatttatttattattatatgtaaa tacactgtagctatcttcagacattccagaagagggtgtcggatggttgtgagccaccat gtggttgctgggatttgaactcaggacctttggaagagcagtcagtgctcttaaccgctg agccatctcaccagcccgagaagctctgtcttagtaggtcaggttttgtgctgcatggaa tccctggcatgggtacagtgtcatgagcgaactggagaaggtccactagagccagtctgt cctgatgatgttggtgagtcctcccttgaaatgcaggagcagcgttcatgcattggaacc cttactgcacatgtactttgtctaactaggaatctccaggttagtgggagcaaaaagtct caaaactttaatattgtatttcttccctgtcttttgtttacaaaggtatatgctgaccta agttataagatggtcaggtttagaatttttgagagcagagttgatccctgacagaacagc tttatgactttgggagagagtttatttctccatctatttccttctctgatgatacgtgat gatattaccatgacctcaaacttctagcttggaaacactgttggaagttctttgcaaatg gtagttgcttccttttttttttttttttttttgttttgttttgttttgttttgttttttg ttaactactagataggatgtatagcttcagtgaagggtatcgttagcctgccttgagatg attacatggaggagaattcatattctgcaaaagtccagtcctatttgggttctgaatgcc ccctaaaggcctggtgcccaggttggtgctcttgggagatagattgtcccctaaaggcct ggtccccaggttggtgctattgggagattgtggagccttgactaggtgggaatgactggg aggttttcagagcaatctcacatggggtgataggatgccagacttttctttctgcctcct ttgcttcctggcatgaggtcagcagttttgttgtaccatataccctgccatttataagat cattctttgttatagacgcaaagaactgggcagggtgaatcttagagcagagcctttaaa ctttgagttgagtatttgctcagctactccagtgacagaaagctggtgtccccctaccat tctgtggttgtgcagatgacttctctcctactcctgagttttgctgtcattagcacaaac ttgggccttgacagtatgaaaatgaagacaacaggatatgctccattaaaaggaaggccc aggattaggatagatgaattcgcctgtgagagtccatgccctttcatatgggttgccacc ccagagaatggcccagatttaagactttccagtcagtctgacctgttcaggcatgcgtct ccagcctctttggcattagctgaatctaaatgcagtcatgttgacaaccgagattagcca tctcaggaacccactggaataatgagttcttagggtggatcttctatgtggacagggctc ttctctcctaattgtgtggtgtgtgagaacctaagagcacttgggtagaatagatggatg attgatcattttgtggaatgggaggcagtagtatagactagtgtgtgatttgactaaggg tcactctttgcactggaaactgagtctcccatatttggctcagacacatacatgctgccg ctttgcttttactgtcctggtaccttgattttagaagtgacaccctagtagaggcccttt tttttttttttttttttttttttgctggcatatttaactctcagaaaatcttatgagtta agagggacaagtttgacttttgtagcagagacaactggcaacctgcaatattttaaggta atttagtcatttactgaagaagtgctggaggcaaggcttggagctggtttttctaaacct tttattgtgctatgctgccttattatactccatggaagttgatgtcataaatgcccaaac atttaaaaataaattggaaaagaagtcacatcttactgtggctccagcccttggcagtga tgtttttaagtagaggtttgtgttatgagaattttgtttcaaaatgctgcatgtgcctct tgtaaaaggttaaatgaagcaatgttatttttcgtaactggcccatatatgttcatttac tttactccttcataactccagctcctttagctgaatgaagcactgctgtacttttattgc agatgccgttattaaaagaaatcgaaagatgctttaatactgaaacggagctcttaagaa gcagttacagttcctagtatagtttaagaagtcacctggttttgtacttgattcataaaa gagcaaacttccttaactttgctattacttgataactattttagtgggggtgggataaat gtcttgtgttttactgatttgatacatattgctgggggctggctttacataatggaggca gcctttcattttttggtatataatccaggcaaagctgcttctaagtgcaagtcgttgccc tttctctacaaagcatttgctgtcttatctgatgcatatgcttagtagtgacctcggtgg ctcctcagacaaaatgccaaaaactgccatgtgatctaaccatgcacactccaggaattt actcaggcatctaagtgagcatacaacagtgggacaataacagtcacatgcaatagggat ccttctatatccatgtgcatcggggtacttttcacaacagccaagatttgaaatcagcgg ccagcagaggaatggataaaggagatgcgcatatatacagtggagttttattcatctatg aaggagacaggcattatctgagttggagaaaaataagataatttgagatgatcctgtaag aaaaacaaacaaaaagaaccaaaacaaaacaaaaaaactaggcatagtaaatttaagtat catgccttttctctcttagtggaatccacacacagaacacagcagtagaagagacactgt gtaggaagagagaggacaaaagagggtaatgcagaaaatacaaacatgccatagctctga agcagtggagcttggtttggaaTAGTCTCCTCACAAACTTGCCAPrimer
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