Human element [ hs1975 ]Position: chr16:53,813,424-53,817,319 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: FTO(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TTTGGTGCACTCCCAATTTactctaaacttctacgggcttccttggagaaactggggcag agatgccgaagactcatttcgggtacaatttagagtttgtttatttatataaatgtaagg ggtaccagtgcagttttgttacatggatatattgtgtagtggtaaagtctgggtcagctt ttagtgtaactcatctgaataatgcacattatatccattaatttatagttttatctcaaa cctcttattaattaatgctagccatggaagctagctagcgcttgtaaatgcctgctgtgt gttagatgctctagtaggtgttttagacaccctcatctaatcttcatggtgattttctga tatatcctgaacctgtacaattttctccacttctgtcactactactacaggccaagccac catcatttcttgcacagacttctgcaccctcaatgtggcctccctacttcaagttttaac agtcttctccctctcataatccactttccagacataagttggatttattttaaaaatata tatcaagtgatgtcacctcactgcctataagactcttcagtgacttaatgacaaacagaa tgagatccttctcctgctctggctgacaagaccagaacaacctgggttctatagctctga gagctcaccagccactctgtccttgctcgttcactgtgctccagactcactggcttgctt tctgtccctggaagccaccagactggttcttccttagggcctttgtaccagctgttcttc ctgcttgggacactctctccccagatctttagtctgggcctggcttcttgtgatttgtgt cttgcctaaaatgacacccactcaatgtgccttccctgaccagctgagctacagcggttt ccctctaccgcatcactagattttactttctttctttttgtttgagatggagttttgctc ttgttgcccaggctggagtgcagtggcatgatctgggctcactgtaaccacctcctgggt tcaagcgattttcccacctgtagagacggggttttgccatattggtcaggctggtcttga actcctgacctcaggtgattcacccacctcggcctcccaaagtgctaggattacaggcag gagccactgagccaccacattcagcctagattttattttcattaaatcgcttgatgcagt gcagagtgagtttctgctattagactgtctggatttcaatcatggcctcgtcatttactg gctgtgtgattttggagaagttacttaacttttcaggactttcatcttcttctttataaa atggaaatattattatatccctccattgtaagaattgaataaggtagtacttagcatctt ttatggtttctgacacattgtacataatcaaaaaatgttaaatattgctattttaatgta atgttattaaaatcattgcatattttatagcttatttatttggctatttttctgtctctt ctaccaggaatataagctttatgagggaaagaatcttatgtgtcacattcccaactgtgc ccctagagcttagaacagtgcttagcacatagtaggcattcactaaatctttgctgatta ttaaaatctgagatataattatccctatttttccaattgagtaatcagggttcagagagg taaagtaacttacccaaggctacgcagctactaaatgctagtagttcctggtatttgtac tgaatgctgattacaaagaccatgttctttctgttatgccacaatacataagtggatctt tatttcagattctgtcatttatagcagatgattttcaggttgcctttttatgtaaatgta acatgactcatgaaatgtaaacagccatttgtgtgaacatgaaaggtcagagccaagagt taggccacaggcaagttaggaaagtgagatatagcacacccaccacattcagtggtggaa gaaaagttacctgattcctgccttggagatgtgtagggtagtgaccttggggttgggctg cctgaggaggttcctatgctttgatttctgaatggacaggatccctaaagtcatcgaccc tagtttgggctcttgtacttcttctatcatcttattctagttggtggaggcagaggttga aaaatcaaaatgttggctccttcagcctctaaaaagtaataggcttgaatttgcttttgt cactaacatgattatggtgcttggtctgtgggggcttcactgatgcattctttttaacag ccctgtttgtttgtttgtttgtttgtttataagtgagcatagcacagaggaaaaaatata ggttttggagactggcacatcagggttcagatttctgctgtgctacttcttggctgtgtt ttggcacgtgaacttctctacacctcagtttctttgtttatgtaatggggattcatatga caattaaagagagagcttgtctaaagtgtcttatgtagtagtatccccagtttgaccctt ttgagaatctgatgacagttatggaaccctctccccagacaagtgcccgtatacccagaa ctttgtatttaatttcatggggttcatgaagcctctgaaactcagcttaagagtccatac caaccaaggtccttataggaagagcttgtgtttttgttttgttttggctttctgcagtct cttaataatgtttattgaatgagagaatttaactaatttccggtttccataatcacttta aactcggtatttgatttccttttccctgggacctgtgacagtgccagcttcatagcctag tctaggcatgccagttgcccactgtggcaatcaatatctgagcctgtggtttttgcctta ggtaaactgtagagatggactcatggaatgcttggaaaatttttcagtttatgataatgt gtaaatgtcgagagccaattattgaggaatggcacctcaaagtatttgggtactctagat cagacatgaccatcttggtgtgtgaaattttgctaatgcatctttcctaatagaatatac aatctcagggctagaaagtcttagagatcatttaatttaattcctccagattgattaagt ggtttgtccaaggtcattcaactggtatagtggcagagctcaggctgtacaaatcaggtg agtattcttcagctcctcagaagccagtaacttccactcacttgtgtgtgtctgaaattc attaaaccttcgacacaaagtgcagccattttaatgctgaatgatattcatcactgtctg aaatgggcttatttatgtacgaatgtttattcctatctcttcctccattctcccaaaatg taaaccccataagaacaggaatcttgtctgtcttagtcacactcagtatccttagagcct tgtgtgtagtaggtgtgtagtgaattttggttgaatgagtggcagttctaataatgtcag aagaattggaatttctttacagaaatctttatcagtaatttaacaaacatcgattgaacc tattatgtatcaggcactataccaggcacaggtacgaatgagatatggtgctcagcttca aggagttacagtctaaataccaaaataacagaatcaagagcagcagactaaattgggccc actgtattttttaaaaatttaatttgactgctgggtgcggtggctcactcctgtaatccc agcacttcgggagcctgaggtgggtgaatcatgaggtcaggagtccgagaccaacccggc caagttggtgaaacactgtctctactaaaaaaaaatacaggccaggtgtggtggctcacg cctataatcccagcgctttgggaggccgaggcgggcggGTCACGAGGAGATCGAGGPrimer
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