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Human element [ hs1952 ]


Position: chr3:186,664,911-186,668,475 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: ST6GAL1(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
gAtgAAGGAAAACATGGCTGCTGTtaatacagtgtgcacagcacactagatactacacgt
agatatctctaggttgcccaacaaccctatagcacaggtacaattatctctactgtacac
atgagggaactgaagttaagggtgagagatctgtgcttgcttcagcagcacatacacgaa
aagtgcaatggtacagagaaggtgagcattaaaaaatagtgatgtttatctgcccagagg
cgcatggtggtgagactgatttgagctccctgtgctgcctgcacagccatgcccatggtt
tttctattgtgctatgatttgtggtgcacagagaccggaatcagccacctttatgaaaca
caaaatgtaaaaataattgtataggtaaatattttataagtaagtctattttatacattt
acgtttatgtttagattggtatttattattatttacatttacctataaaataaaacatgg
tttagatgtaaattttgtaggtaaatttttaggtaaatataagagcctacatgatggtcc
agactccaccatccagcagccgtgtggccccaggctacttaatcagaaccctaatttcct
tgcctatagaaggggataataattgtgccactgcctagcctgagattagtttttatgttc
aaattagatcatagacgttaaggatgagacagtttgaaagaggcacacagaagggtttgt
tattgcggacacaccccttgctctgctgagagcatacatctgttcaagcacccacaggtg
tctgtgaaaatgttcacagtcatagacacagcccctacagggttgacacaaacaacctgg
tcaacaggcaagaaactgcttccctgactcagttaaccagctggaggctgtaatgaaagg
cagcagagagtgagggagggaggaggcagggagtgtgtctggctctctccctgcctgggt
gagaatccgctcttgcctgccttcctggttgctggggaatttctgggccagagttgcgtc
gggatggctgtcaacagcttggtgagggtggaggaggctgttccagacgtgccaagctac
aggaatgaatgttggagctgccacgccggcaggccctgccatgtctggtagacgccagtg
gtctgaaagagggaccaggaaggatagggttgtcaggggacttcggtggccagctttatt
ctccagaagtaccgatgaggacaaagcaaggccctgacctcaaagaaattgggcttctga
caacacttatcaaactgggcgggcagccagacctctgctctgagctagtgtggctggagg
cacctgacgtttgctccgggaaggatcaggatggttgtagggtgtgggctggggcctggt
atgctcttgggggttcagaaactctagtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgt
gtgtgtgtctgtgtgtgtatgtggtcgttggagagagacccaggctattccatatcgctg
aagagatccgtttcttccttatctcactggtctctctgctataccaacacctttggctca
gcctcagctccagccagcctcagttccggaggagccccctcaaattcatggcccacaaag
gagagagaagacctccgatctcttgtgccatgctcactatgctatgtgaaatttgctatg
ttagaccttgacatcctggatggtggggatgatgactggtttctctgcacagccctgggc
ccagtatcctgcccagcccttaggagtcctcatggaatgagctcagctcccgcgatgctc
ctgtttgtccactgaggtaagaacacatccagccaccagtgagttgagtgggtgttctct
cggtaagatcaaggtgtgacagcaggcagtgaatttgcagtagcccgtggtgggaagccc
gtgaggctgtagtgggccctcctgctccaccagagagtcatttttcctacctgccaattc
tagccttggaaataaaagccaagcaggcgggtgaaagagggaaaattctctgggatggtt
catggatctgagccctggaggagagtcaagcttgggaccttgttcttcttgtcttatttt
gggaaccagggtcataggaatttgttccaggtgagagagggaagaaggcagctagggagc
tgtgacgtggcagccaggctgagttatcagttacctaagagtctgcctcagggattgcct
ttgtggactgcacagtgctggttctgggaggaggctggccttgggcctgagctggaggtg
gtggctgacagtgtgaggaatgaatccccagaagatgattcctgccagagggcttgtgac
ggctccttttttgcttttcacaatgaacatcttattctgggctgcaaatttggatgaggg
gaaaaaactggccaggaggagatgaaccaaaggcgtctcacagggaatccttctgtactt
gaagagtcctgcccttgtctccccagccagatccagtgactcacagtaggagggcttgga
ggcaactcactgccttccctgttaactaccgtttgcaatgtcaccttcctcagaaagacc
ttatcattcggaaaaaaaaccacatgctttgtagccagacagacttgggtttaaattcca
gatatgccacttctcaggtgtgcaagcttggcagcttggcctctctggtcctcagtttcc
tgtcttagtctatattcatcctttctgccttgtagcattggtgtgagtcctagaggtagt
gcctaacctagttttgggcacatggttggagaccaataaatcgtggttattttcaatatc
acagcacatgtgaacggttattctcagttattgtccacctgatgggccactttgctaggg
gctgtgaatagaaagagaaatgagatacagcctctccctggaggagctctccatctagga
ggagagccaggcctgtggaagggggcacaaaggaaagcaagttgcacaagctgtggaggg
agctggaggcaggggcagagaaggccagaaaaagagatgatatcctcaaaggctttaaaa
gaatgaagaagaatctgccaggcagttggggcgggggcagcattggatccactcataagg
gagggcagagctaacagtggaggctccctggacaggtgagccctcgggcttccccctctc
tgctggcatttgatcctactcagtttgggtcaggatagagtctgcagtcattttccagca
ggtggttttacatgggcaggaatccagccaggcctgactgggggagagggtgttgaggtg
gcacttgtcatcccagtgatctctccataggtatctgggctccaggcttagggctaggag
caggagtgggcctcctcagagggagtgggacagattaccaggcaggaactgcaaaggcag
agctcactaaagtgcaggctccaggtcatgtggatggaggggcttcacaaaatggtacaa
agcggttgttatcaaattcagacagcaggtaggtgggaggaggggtgacctgtgacttct
gtgatTTCTGGGAAGTCCACTGACC
Primer
(+)caccAAGGAAAACATGGCTGCTGT
(+)GGTCAGTGGACTTCCCAGAA


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