Mouse element [ mm1924 ]Position: chr13:15,718,380-15,724,077 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TGAGCATTAGTTTCCACAATGGttgagtgttctcagggcacagacttctgtctgcctccc tgtcaccattccgtttgacagtaaaagatccatgatttgttcagtagttttggccaacag acttacttcctgcttcttgtgacctctgatattagtagcttggtaattcctcctgtgtgt cttaggatcttgggcttgggcctccgtggtgaagaattcattgcctctcagaacaggctc tcttttcctaacattttgtttgctacatgatctatggtaatgtttcgttcctgggtttca gcatataactccagaaggttcaaacattagctcatacattctgattatcttcatttatca tggtgtcactgggtatccctggctgatctggaactcaactgtgaggaccagtctgccctc tgagtactgggattgaaggtgcttgccaccatgccaggcctgacttgtattttcacggtc cctgggcctgagctgggttccattctgtgttaagtggctccttgagtgttttcccccacc tttgtttccagatgcaaattggaatggagaatgggctgttaggggaagatctatgtttgt tcattgacttttgttgttttgtttgtttgtttgttttgttttttccttttatacagacac acttaaagtgattctgaagagattctctaccccatgcatgctcacatgaccttctgtttt atcccataagccataggtttcctgcagattagttaatctctccactctgctctcactatt tcatctttttttttttaatctaagatccaatgatgggcacacatagagcacctaatgtct cacaaaccacagagatttggcaagtggtagactgcccgatacatgtccaatcatgaactt aactcatgtttgcagctctgcatctttctcctgaccctggaactttggaattggggcata ttttataaactgtagtggtttctgtttttattggtgaagtggcacgcaggaggaagtttc acgtgtctcttaatatgactgtcttgttttcatttccatttttgttcatggactatagtg accaaaagcttttaaaagaaagactatatttgagaaaggcttttagagttcttttaaaat taaagcaaaacctggaagtgagacaaaaatatttctcaaaatcctgaatctttaaagtaa tgtcattttagagttgaaaactcatccttgtttgtttttaggaaaaaataataataaatg ttttcttccccactaaaatcccagctactgtttcctaaattacacagtaatgatcctact tggaaaattgcaaagttaaagtatagagagaatgtttcagaatgcttctgctgcctacat cttagaaaatgaaaaacgaggggtccctcagcctggcaggggataaagaggaaggcatgt acagcgagctgactgtgcggtgtgtttgtgaagatgcatagcaccagctctgagagggta aggcttcatgattaatggtgtttattcttgacagaagcctcctttatttatctgcaatac agtttagcgattttatcaggcacctgaatgaaaaatattcagtcataccaatattgcagt gtaaattgagtagagtcactttttattatatctttttgccttatcatccacacagtaatt ttctagcagaggtatgatatggaaaggctggatttagtcgtacttgacacaaagcctctt caatttcccctaaaagttcaggcttagaagtgtgtgtgtgtgtgtgtgtgtgtgtgtgtg tgtgtatcttatatataaacccttctctcttattgaagcagtttcaggatttgaaagaat aaagataaactgtacaaacctgggtagggaaaactgttaatataaaaatatgtaatagct gtaaactggctgacgcgccgctgctgggtgagctcctggactccacactgaagctttgtt tggctcctggtcccactcttctaatcagccaccatcaggcctcatcagcataaggcttta ctctatcatctctttgggaaatgacatcaaagcagagcatgccgctgccctagagaggac attagtagctgcaaagatggcagtgctgtcacagggatgaagaaaggaaatggagaaccc attttctgactaacgatggagccttgctcttctggctcagaagtccacctaaacctgact ccagtgtgaatgtgtgaaagtaggcgcaacatgaggtcaggattaacttcttgaactcta gaattgtgcttttaattattgatctgtacccaaagtgacttctcacacaaaacaaatcca aggttattgcttctcggatttttaaaaatatctatttgtctaaattgtttaatcatccag ataaatgtacaaccatggaatgagttattttttaatgccttttaaaacattctttgtata ttttttattattttcttttatatccctcaagtcctcctagatctgtcccccttcttaccc acacaactctattccctctcttaaaacaaacaaaaaaacaaaagaaaaagccagtaaaaa gcaccgagttctagttctgtttctgttggccaactgcttctgagcacagtggagtgtggt tgataatgcccagtgttactcctttgaagacaaccaattttccctgcctcgccctttccc agcctcgcttcctataagctatttgtaaatgttttcttggctggggtgggagtttgcacc cagtccctttcgtttatgccgagatttctgtctggtttgagcttttgcatgttttgtaca tgctgccatggtctctgttggtccatatgtgctccagtgttcttctacccagaaaagtaa atgtgtacttggagtcattcctcttacagttagtagatagcatgactcaaactcagactt tctacaaaatatgaaggtaggcttctttattgttatgaggatatggcacctaaacaagta tcagaaatttcagttatagttagcgctgagatggtacttggcaatttgctgggtgactac atgtttcaaaatcaaaatatatatttttttgatgtatattttctttcggacccaggatat tacttgccgtttttatattttaaatataattgcctattgaatatatttgtattgaatata ttttgaccatattaccaactccttgtgtacccacttctctctgttctcacatttctgctc catgctcgcctactttgtccctctaaaccagtggttctcaaccttcctactgttgtggcc ctatggccctttaatatagttcctcatattgtggtgacctcagccataaaataatttcat tgctacttcataactgtaattttgcattatcatgaattgtaatgtaaatatctgagtttt ttgatggtctcaagcaacccctgtgaaagggtcatttgacctttaaagggatcagaccca ctagtgggaaacagctactttaaacaatctctctctctctctctctctctctctctctct ctctctctctctcgtgtgtgtttgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtg tgtgtgtgtgagtgagtgtgtgtgtgagcatgtgatactgttactattagaggaaaaaag caggagaagcagtctaagaagtacgcatagacaggatgttctgaacggaacctttgttcc tcaagacatttttctaatttgttggcaatgtcttaagtctttacaataaaatgcctcttg ttttttatgcccctgattcttagattgagattttttttcatgatgttctattcatcttga agttagtactcatacttggttactaatttattaatttatcttttctttgtttgagtgtta cagttttctaacttgttttctgtcttcagtattctgtccttcagtcaattccttggtcaa ggcttttatattttaggtaatgtgtttttccatttaaaattttcaaattattttcttttc agtatttatacctctttagagttcatcttttatatcttatattgtctttcttactttccc agctgtttgtggtctattgggattcattcagaaatttgtattttctttgatttctctgaa cactcctgtaatgacccttctgagctctccaggacagaatgtagttcacctgtagttcat gctttgggataagtagttttgaggagcagagctgctcagtttcttgtttacgcatgtata tgcattaaaggttgtacataatgaagttagataatgagttggatgtatttattctttaaa aaatgtattcatttgcttatttattttaaatcactctgattgtttccaaggggatgcttg ctctgttcagtagggaccacagggttgtagagttggggtgcaaattagaatattgtttgc aaagacaatcactatcaacatcaagccagcaatgatggaacaataagatttaaatgaaaa attaatcctaccagaaccaataatttaggggcaaaggataactaaaatactgaatgcgct aatacattatttattgtgcatatgtatggaaatgaaagggggagcagtcccaacaacaca acaaagaaacctagggttagactgaggtagttcttgtctaaagcaaagagtagagtagaa aatacctggaagaggaaacggttgccgagaagtagagggtataaggaagtcttagctgga gaatgactgagtagagagaagtaaagacagagaactaaatgaagaatgccctccccagtt cttaaggaggctacacattcctcagtaactaacctgccctttccagtgttgagataatgg aatagggaagagcagggacacatggtggagtagggtgttgcttgagtctgagtgactggc ttcctctgttgcctgactcctgctctatagttctgctctggcatctgtacctttctttgg gacatccggtggcagctgatattctaaacaccttgtagtataacagaagtcacatgagca caggaccagcactaacaactggagaccaatggtgtgacttggcaacctaaaccccacctg tgaagtactgtctactgagctggctggggtctttttgaaacagtatccttccgttgttcc aagctgagtctacttcttctaatgaccatatgttcatccctgtttcaagccactcagtct actgtgccacgacgccttattgcaggactaatgatctactctatgccccgtgcttcatga cacaggggccaggtgactgtgctgctcactcagcaggggtcctttccagatgagacccac agctgttttgtgaatgaaagcacagcatatccaccccttgctgaattttccttttcctct actctttatgatatcacatctatggctactatacagatgtctttagtctgccagtcatga gctttgtctcctagagttcgctgggtggaggtcctttctctgtgtcccctctaagctggc tctagactttctctcctgctatagaagttctgttttttattcctcttaagcataaacatt catgtgccatctgacttctttcaccattattgtctacattttttttttttttttggagaa tctggaagactatacttaggggttgttaactgtcttCCTCTCTTATTTTGCCCATTTGPrimer
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