Mouse element [ mm1922 ]Position: chr13:15,705,489-15,710,589 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence CCATCTCTTTGGTCTCAGCTCTtgaaattcctaaaagagatttcatactgtaaagttgcc ttcagttgaaatgccatctcaaagtgttcgctttatataaggtgtactttctttcctttt cagaccctcctcatcttttccctgccttccatcctcctgtaccaattgatgccagacatc atgagggccgttaccattatgatccatctcctattcctccattacatgtgtaagtattaa aatgttacccattaatgaaatggacctaagctctggcttttaggcttcaggactctttgt taatgcatgtcttctgggctttggaggtgtgataacagtgtaagatttactcttaagata cagcaaagctctatctgctcctggagtcactgaatcagaccctgtgctttctgaggatgc ctgtcatatggtttagctggccactggtttctcagtattttgctgatgagagagagagac aaacagttgtgagttataaacttaaaattaaagcattaaagttaaattttaattgaagct taccacgtacaatatgtgcttggttgtgattttggtttgaagttctacatgctaaaattt ttgttacatgaaaagttctgaccaaggattacactggatcgaaatgaaatgtttatggaa gattttcagaattcccatggtccatataccaacagtcacttgattgatgggaatgtcacc caaataagatggcaaatgtgattgaacacatggtttcacataagataaaagaaaaggatt ggtgataaaatgtatttagtataaaaggaaaaagaaagcctcattgaaataaatattatc tcagtttgttttgaagggaaagaaaaagaatcattgatttatacactaacagaatattct tctgctgtaaacttgagaaaactgtcatgtaagaattggatgcactccctggaagggact gttccctgctaatggcctcggtacacatgtagtcatatgtaataatcaccttccttttga gaaggggaagttagaaaaacgcgaataatactttgaagaataatgctggttatatagatg ctagattgcatctctctgactattagttacagatgtgtaattacatggtatgccgaagaa aaatgaagttgttttaaaacaagtcctcctctgtgaacagaattaattgatattaacagg cctaactgtatgcccaagtacacatataatattttaccaaccggtctttagatccatctc ccattactgaattgtaatttcagttcagatttgcctggaggtgagtaatgaatctgcatt gagaacttctgtaggcattggaggagaagtgggtatttacagagggagaagtcagagtgt tgtgacttattcctcctgtgacttctactagagcacaagcaagtatttacaggattctga agcctgataaaactgcactcacctaacaccaaagaccaaaggaagttctactacacagag ggaagtgcgttcctaagtgagtttgcaagatgacctgtgactcacctggtcttatagagc ctttatggcctggataggaccacagagatcttggtgttctgtcctgtatctagatcacac aagacctcagagacaaagatagctaattcctcccagtctttctgagaaagtaattttaac cagatgttctatggaatcggaaagtttttcactttttcaaaaagcatgttctagagaatt ttcttaaactgagctcaaataatatgctgaactctttcctctgggcttcttatagttcat cttttaagtaaattcaaataggaagttaccaatgaaaaactgcacctcttggtcctctgg cagagttataggaccgtcaaagtctcccgcaatcctgttttgtgtcctaggctgttaggt tcatagcaaaagcagtgtcacagatgagctaagccacgtgtggagcagcagagtcggccg tgggttttggcttcagctgtgacattgagctggggttctaagcttcacagcacttcctgc cttttcagtcaagttttaagatatttaagcagtgattgactaaattgttcttgtgcagcc tacatgtgtaattatcaaagagttatgatgtaaggcaggttgacaattcaagtttcaaga gaataagtccttgaaggctctgagagaatgttgtgtctgtgggtgtgaaacacggcaccg aatgtctgcagtttcctgggactgttcacatcataaccgagtagattcctgggtggctct gactgagattatgacattggctctaggaagttagagcagtcacatctccatgcctttgta gcattgtgctttcttcgcattgttttcttctcgcttggtaatttcttaggagctttattt ggggaaagtgctcagcatatgagcagatagacatgtagatgctgctcagtggatagctag gctggtccagcctttgaagtattgcctgagttatcaggatgccttaggcttgccacacac ttgtactgtaggccctggcaaatgccccctgatgtgggccctgcacttcactaaaatgaa gctatttgctttaggaagagctcaataaaataacctcatgtatttactatactgtattta tttgggtctctaaataaatgcttacagttagtgtataatggcaccaaatttcttctagtc ttgctccatgatgacgcagctgtctgtaccccttctgtcaggcgtggacacacctgtgat tgttaaagctgagagtattgatagtaaatgtggcccctttacccaaacctccccagtatt aactgtaaaatataaaagtatcctatccagactttgaattccaagatgtttgctgtataa ttgcctcctagaaaatgaaatcaagtgagcttactaagcttttagagtgaatattttaat tacaggaagataaaacttggcgatattctcattattttgtaaacacaattgttcttctgg tgccaactttagaaactaaattataagtctctgaaaaacaagggcctgtgttgatggaaa tgctgacagaccagtgttcagaaggatagcaggcactgcggtaattatgctatcatattc catcattagagattctattgaaaaggtttataaattgctcctctttttagataaaccacc ctatattaaggcatcaatgatacggtgcagaattaaattaaaatctcataaaatgaaata tgttctgtattagctgtttgacacttaatttgcctctgaatgagtagaggaaatcacaaa ttaacctgcttttgtttttccaaccgtggcttattaggtgttagaacaactgcaattata gcccaataataacccaggagccttataaatatgtacggaaatatgtaagcctgtgtttaa aaactaggtttgttacatgaaatgcctttagttacactccttgcttgcacactgttgtcc acagtttatggttgagcaaagcagctcgaggatagacatttaaatgaactaaaatacagt actagaaataatcctaggatttattgaaatttcttttagcccaaaatcatgaaattaatt ttaaaaggtcataagtgggtcatgccataggtgccatgtctctaggttgtcacatcaaac tgctctctgaaatggacaatgactgcatcatgtttatgtcattcaaatagtttcttgaac attgaatatgagaattctttcctcatcttatgagttgttcagaatgcatgtgtccaactg atacctggaatgtctctttctcaggttgtaataatccccaattctatgtaaagttaaaca cagtgttttagaaaaatcaccaaagtccacttagcatattgctgagcccatctggtgcct ctgtgatatccctgagcccggacacacactgcagaagagaacgggaaacgtgtccagtcc tctctttgttccctggcttgagctgtttacaccagggctctataaccctgtggtcacact gttctatgctgtgggccttgataaagccctctagactacatgttactactgggttttaaa acttctttaaaaatggagacagttgttcagttatatcttactatactcagattttcaagt gattttttttttttacagccacaaagaatgtgctactgcattttgagtttgtgaacattt tgagtttgtgtgtacatttgagtgtgtgcattttgagtgtttgtgagttttgagtgtgtg tatgttttaagtgctggaagttacttgatcatgtttcatttcaaaagtaggcatgatgta tttttagaaactcaaattataaagacttttgaattctagtatgatttcattcacattctt gtggggtttccagggtgtgttgatatctattctgtatgtaaaaactctttgagccaagtt tttctcattcatgaaccagtatgaattagattttggactttcctgtagctttctactgag gtctacaaattcattttccaaagctgagtccttagttactaatccattgcaataattcag gccccagtgatctgatcttcttgctgtgaaactgagagcagtgttggcattgtgcagtgg atgcgaatggtacgtcactaagctgcatctttagacagtatgcagaacctgctctgcatc ccctccactttactaatgccttttagaaaaactgctttatctttacggtccagtgagaag tgagcactctcctagttctttcccttgagtcaaggtggcttacagggaagctgcccgatt cagtgcagtggctatcaggaaggggatctagccttcacaaaaactagcctgcctccagct gctcagagaggagtgagggtacgagccttaccatcagctttgagccagattacactggga caaggaagttttgcccttgtgattacatagccttttaggcttgagagcagaaggtgagca gaaagtgtgggaaatgcttgcattcactacttcccagagagagacagagtgtgaggaaag gtgctactccattatggtaaatatctagggaaagcaacaGTAGGTAAATGATCAGGGCTG CPrimer
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