Mouse element [ mm1918 ]Position: chr13:15,676,239-15,681,038 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence CTGTTGATGCCTACATTTTGGAatagcctcatttttcagttcttcagtcttactgtctta ctcctgctttgttgatcttcttgaaccccccaccctccccatgcaaccttaaaagtgctg gttctttgacaaccttcctagttcttggtctggcctacagcagccctgctactccaaact tatcagcactctgctttctttgggtcgtccgacttttagtagatgtgtctttaatgggga gtatcagttacccactgttgaatgaacaacccagacaatgatgtttaccttgtggtgctc tcatctggtctggtgtttgcttccaccaaccacatctggaacatgtgcatattctaacac aacatctagataggtatgtagcaaggtctccatgttaggtctttgccaatccacatctcc taggaacccttcccacaaagtttggtttggatgatacttggtgtttatttctgctagctc tgtagtaataccttttcctattacaactactaccaatagtaacagtgtagtaatctaggc ttgaaggtcatgaatagtgtttcagcatttccataattcttggtaaagttgagatataac ccaagtgtctgactcccagagctgtgccttgctggactctgttgctaccttgcctacatt gggtgcccactgtgctctaagtaataaaatggccttatctttaacagaacaatttatatg tatacacaagttcctttgtgaatttgtgtgcaccatgtgtgtacaggtgcttgtggaagc cagagagcatcagattcttgaggactagtgatttgcctgatgtagagacagagtgctgac tgagtctgctgcatgagtgttgaacccttgagccatccccacccagctccagggacttta cgtttcaggtgtatgcctatgaaaagaaacaaatccagctggtgtgattgctcattcatt ccatgtcagccccatccttctctccacccttggatggacggacttactttctttcttcct ttcttccttccttccttccttccttccttccttccttccttccttccttccttccttcct tccttccttccttccttcttttcttttttcttttttctttctttttttttcaagacaggg tttctctgtatagtcctggctgtcctggaactcactctagaccaggctggcctcgaactc agaaatccgcctgtttctgcctcccaagtgctgggattaaaggcgtgcgccaccaagccc gaccttggtcattcttatatgtagggccagtttcctttaaaataactttattttcatagt ggtttttaaatgtgatggtcagcagcactgagcatttacaaaggaagtagcagcagcatg aattgctatgtggcacactttccatggtcctgtgctgactctgtgatgactgggtcacgc tggctctcttcacacctttgggcttttcttcccttgcactttgaaccttcgttaggtaat tgtgggtttatttgatatttctttctttgtggaagggagcaatcacctttgtgctgttga tattgcctctggagtaactagcagacagtaggaattcaatcaacatttgtagaaattcag ataagcaaatgaataaatcctgtatgcttaggcctgtattctcatttatgtatcctgtgt tagattttcgtgaaagtctgtggcaaagagattgtttttctagtgagtcatatggtaaaa ctgagaagtttattcatgtagaggaattataaactgtggacacatttggaagacagatga agacagaagctagttagtttccgtcttgagcagcaatgactatggctaccttcaggcttt agacttattcttgctattagaataaaacaagaacttagataagatagctctgcatattcc tcacatttcattttattagaaaagtggtacttgatttttttctcagaaaataaagtaact cagacaggcagagctaataccctgtaaaaaaaaccgctgtacacaatatccagtgtcact tttagggtgccctttttgtcttttccctgataagccctatggttttgatgtgtaatttat acctgatcaggagcacctcttcatcctatttattgattcttccttattctgaaacctcgt tttctatactattaacttctgtttcaggaaagagatttttattgatgaagaccatttcca gaatagggatgtggctcaatgggtcagtgtttgcctagtatggctaaaactttgggttgg atcccaaatgggatataaatcaggcctgtggcatatgtatttatctcagcactctggaga tggaagcaagaaagtcaggagtttgaggtcatccttgagtgtgtagaaaatttgaggcca agcctgggctatatgaaaccagggaaataggactaggtgagatggcttagccatctcgat gccagtttgatgatctgagtttgctccctaagacgtgcatggtagaaggagagaactgac tcctatagattgttctttgagcctcctgcagcaggtgctctccattagacccaaagcctt ggctctttctgcctgagtgtgtgtgtatattactttttacctgtcagttcagatatccat ctttttttactgtggatatctaagtttctaaggtttcctcttggggccattgggccatat ccacttggatatctgcaatatatcattatcttagtacagcccatgatttttgtttgtttc tttattgggtgtttgtgaggtcttgtttgatttttgaaacaggatttctttatgtatccc ttgatgtcctggaactctgtagaccaggctgaccttgaattcagagagccacctgcctct gcccctgaagtgttgggattaaaggcatatgccactattcctgactcttttgtgtgtttc ttaaattttcgacggtgtcagtgtcacacttttaggaattccgtcttttaaaatctgggg tttactctcagtgcaagcttcatcaggctttcagcagctccgactacacctaatgtgcac agcttcacgcaaaggacaggaattgcggcatgggatgacgatgctagagcaaagtgcttc tggtttccttggaattctggcaggaacagaaattctttcatttgatgcctgaggtgttgt tcccttagtcttttagggatattttctcaggttaaaccttcatgtcactaaagagaattt caggataagccagtatgaagcagagttgtaaagtttactgagaagtttgatgtagatttt agtgggagattaatagaaaggcgctaggaagaataatgacacatactggagaatctgggt gtggcttttctttgccaagcatactttatagtagctctttacactgtgttggtagctttt ggagttgcatctttaagagtggctaaggaagctaaatgcaattatgacgtggttcctatg cactggacaggattacagctgataaggtcacttaggctgcaggggaagctaagatgatct taatgtaaataaaggtctagtcttgtttgtgagtttcccacaaaatatctgggaaaggag ttaggccatatctgaaggccatacattcttataaccttatctagattcattgctatttca attttgtttttttttcttggtagtcttgcagcagatgctgttaattgcactgtgattcat gatttcggctgactccaaagtgaggggcctcctttctatcccgatgaccccatactattc tttgttttttattctatctcaaaacaacctaactatgagggttaaatatcagcgtatgct ctaggccaaatcacatgtctgtcaaaggatgggaaggacccaagtaggtacttgacagtg ggcggtttaatctcagcaaactatgccaaagaggctggtccccctagggagtgggtgtag ttttctggctcttatagtacttccgttcatgatttaatctgaagttgctggttattatga catttcatcctcagttcatgaggactgaggaaggtcagtcgaccctgtctgtggaactgg caacattccattaagcagacactctctccggctcaccacacagtcaggtctgtgggagac ttgatgttcccattagtcatccagacgcttatatcatgtggacgtcacccctttacgtcc tctgaccccaggaccccctgatgacctttcacttcattttcaaccctttgaggccggaaa tgtgggaaagcagtggatgatctacagctgtaaagtgcaggaaccctgtcttgactttca ggcttatcctgaccaaaagcgtgctatgctcctgaggaggaagagtgcaagtgtctgctg taccccactcaagaggcctctttttttttttttttttaaataacagtaatgaaaaaatag aaacaaaatgtacttcaggttgcaaattctgagcagcatctctgaactcacagacatgct gtggttgcagaatggttgcaaagtgtctccagagatagcttcgtaagcttttattacacc tccggccattgattgcatggctttatcctactctccaatgtggaagaaatactccacacc tcccattcacaaaaaccctggatcagagccaccttccctctgccttctgctggtgccagc tgcagctctgtctcctggcttctcactccttcacagccacggcgcacttgcacttcctag gccgcaaccagtgagggttctcgccacactcttaacttCTTTCCTGTGCTCATGTTCAAGPrimer
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