Mouse element [ mm1908 ]Position: chr13:15,610,282-15,614,935 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GCCCTTGTTTATTCCTCACTTGacctctttcctttggccgaccaatgtccattgggagaa aatattctctcagtgtgaaatctagccctctttatgtgcaggttattttgttacattttt cttttctatttatttgttaattaattattgattatttttgttttttatttttgtttgagc ttctgttgtggaattttcattctttgttccctctgtctcttacactcatttagctctgta ggggctgaaattccttattattactaatttttttattactcaattgagggactcttgaag tctttgctgacacccttcctaacctctcgtcacacagttcccactattcccatctatagt gcactgaacatggcttttcccaggggcctggaagccccctcatggtggaaatggattctt agcttctgtgttaacacctttgtgcctaggaaactgttgatgagtctgcttggccttgac ttttgtaatctggctatttaggagcctgtgggcagagagaggagtacctgggctctttct taagccctgggagcatcctgaagcaggagctaaggagtgtgctattcaagcagatgattg gtttccttcctttccctaaggatccatcgcagagagggtttaagctcgttcaagatttgg tagaacatacctttatggaaatactcactgaattaggttttaggcctttttttcttttcc tcaggctaaaatagaagattagcatagacacagataaagtgagcagactgcctaggctaa gctcagacaggagcctgctttctgagcaggagatggggtacggccttccgggcatcagtt ggcaggtgtacatgcctgtattgtttctaagtacgaaagcatagagggaagggaaaagag tgggagaaagggagagagggagacaaaagaccaacaggaactccatgttcacatgtgttg ttgccatgaaggattttgtgtacttaaacaatgctaggacccatcgggacgcatcatgcg gaattgtggagggcagtctctgcacctctgcttgggatcttgtgtctatgtcttgacgaa ccttggaagatatctttgagtagtccttgtactacttgtgggtcacacataatggtaatg gtgctgaataaatattcgatcaaattgaaggctgattggctaaccccttgtttgtcactg tctttgtgtggccatctttctccaccatcatgtgacttgtaatcttgttgacaaattcaa tcagtacaaatatgggggtggacctgaagactgtgccactcaacttatgcaatataatcc atagatgtgagcttattaacctttgactttgttaagtaaatttagggaacgttcttgctt tccctaagcttttgttgtttttctttttatattttatattagcaaaatgagaataataat gtatgtgttgaggatttaaggatatcaaatatgcagaggggcttgtggtttgacatgcag tagctattcctggatatattcattatggtatacaactagaatcatattctcatatagcaa tctatttttaaacatatcctttctatatatattttataaaacataattcagattcatcta tttttataagccatgttcctatttcctgtattttataaaatgaaacaggattgcaggggt agactttgtttacttagcttgtaggacacaggagcacacatttcatacactgttggccca gtgagtgcacttcctttgtattgacctctggcgtgacaagtacacagtgacatggccgac acacagctgactgaagatcatacatgtggtatgcttttgatcagatagtcacaaagtctg ataattcacaaagtttcatggcgtactgatatcagatagcaggctgcataggcccagatc aggtgagatcccttcacagacagacttcctgtcagcatgggttctgaggctatgctgtgt ggacatctatgacatgctgcttgtgtgtcctcttttccccctcctggcaaacccactact gtctccatggcctgcagacaaggaaatgcactctcagagttcagtgtctggcatgagtcc tgtacagtggctgccaggtcttgaaccctggcagtcatatggcatgccctggctgtcacc ctctgtgtagcctggggagaggttgatgaatacagtctatcaaaaggagctgtgacttgg gggatgaaagaagaggtccgtgaacacacggatgttcttttgttagtaaattttgcctta gttgtttgctatatgtcagggaagagccactgtgaactcccagtttctctggcagagctg atttatgaggattttctcagatcttccagcaggttaacaacgtggagtcttgaaagcttt caagaggggaaatgaataaacaggtatttgaggtgaagccgaccaagtctgtggagctgc agaaaggtgaattaaatccgttgaaaaggcttggataagaatttcacatgtctcttgacc tagtaaatgagagtcaggaatctgactttgtaggtcagcttcctgattcatgtagataga acttgttagaagaagttttatttggcggagatgttacatggctttagtggactaaatcag tcaggaaaaacaaaatgaactgtccagataaaatcagctgagaggcaatccttacctcac ttttaagttgaattacgttgcccctttgacatttttgaacagttataatggttatatttt gcattctatcctgttcttgctttatacatattcatgttttcttttaatccatagaatacc tgaaactatgttacactgagttttttaaaagacaaacaccagcaattgtttactttctgt ggcctgttttggccctttggaagtaatataaaacacacgcgcgcgtgcgcgtgtgtgtgt gtgtgtgtgtgtgtgtgtgcctgcctgcgcgcacgtgcatgcatgtgcaagtgtatttgg tagaattaagagtgtgtcaagatttctataatctttcaagcactcagaaacaaaaatttg tggtgaaggtcaggtatgacctgacaaaccgagactgacaagctttggtttttttttttt tttttttggggggggggattgtgaaaatgtccatttttttccccttgtttcccatgtcac ttggcctgtggagttaacaggactcttctgaggaccagtggctgcattgctgtggatggc actcctggagttttccaaaattcttttttcctcaggggaatgagtgattcagaacatcat ttgtaaagttccaggctttgttcttagagttttctgacagtccatttgtattcattattg atgtattcaaaactcgtgaaaggagcctgggagattgagaaacatgcttataagcaggat aaatgagcaacttttgacagaatctaaagtgcccaggcatcctcacccttggagggtctc caaactctgccaagggctctgcccagacattgcacaatgtttgcagaggtgggaattgat gacaaggaaggcagtataagatcccattggcatcatggtggtctacccaggaaaaagttg gttccaatacgaggaggggaagagtgtgaaaagtgttggaaagtgagtgtctggggctct tcagttgattgtgtagaacagctcatacccatgtttgttcccctcagtgaggttgaacca gcctgattcttaatgaaggctccatctctccatatcagccaggcattgggtattccatga atacttaccatgcctcttttccagggagggtgatgcatacgagcattaagtgttgtcttg acagtttgacttgccaggaagagaggagtaccaagtggaattggctgaattcctagtccc tgaagtcagggtgcagctgtccttggcaagggtgctgtagaccttcatgcaggtctcttg cccgagggtatctcttttgaaggtagagactagtaaatgtctttcatgttttggtgcctc agctttcacagacacaaaaggtgtcacaatggttagcgttcacaaagctcctgtgagaat agcagaaatagaaaagggaaggtaagactctccacagttactgggttagaaagaagtgtt actctaagtggtcagttgaattcattttatttaaagagaactctgaggtctactgctact acaccacagcccactcacaggcgacagttttggaggtcatgcccggaggctaagttagga cagtggagaagccttggccttgggcatttgtcttcgttcgatttgctttgctgtgataaa cactatgaccaacagtaccttggggaggaagagtttatgtggcttacatgtccaaatcac agcctgtcatggaggaggtcagggtaggacctctgcaagagctgaggcaggaactttgta ggaaagctgcttactcaccatggcttgctcagcttgcttcctatctaacccagacctctc tctctaacagtggcactgctcatgcggaccatatactcacatcaattactaaccaaggac attcccctgtagatatgcccgcagactaatctgatgaaaatatttctgtaattaaggttc cttcttctctagGGAGTTTGTGTCAGGTTGACAAPrimer
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