Mouse element [ mm1907 ]Position: chr13:15,601,775-15,606,229 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Gli3(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TTATGCCCCTTCTGAGTGTTTTctttgttgtgaattcatatatttagtcattagtcttat gtatcagcttcatcccaacatttacagagtgcaatatgggcttgcttagcatagtttcct ttattacactgtcacgctgttccttacaataagaactcctttgttattgtgatgcaggat aggtaagaaatatttgaatgttcgtgttaattacttccagttctcaaatgtggatctggt gtagaaccagttggagcaaatagttcagcagtttacacctgggatgtttgtctagggtgc ttcagtcagtgtaactcgctcagtataagagatatgttccttcaaatagctaagatctgc catctgcaaaaggtattatcccaccgctctggtgaaccgcctagccacagtgctattcct cagctggcttccgcctagccacagtgctattcctcagctggcttaattgccccatctctg tgtcttcctttggatggctgtatagctttccagtttctttcttcctttcttcctttcttc ctttcttcctttcttcctttcttcctttcttcctttcttcctttcttcctttcttccttt cttcctttcttcctttcttcctttcttcctttcttcctttcttcctttcttcctttcttc cttccttccttccttccttccttccttccttccttccttccttccttgcctcttggtaga acagtgttgcttggaaaatgtcagaaggtatctaaagaaccccttccccaatctcttcaa taacacacacacacacacacacacacacaccattaacaactttggtgttttgtttttgtt tgtttgtttttttttcccatacatcttatatcagattttatggttgagaaactataaaca tgtttggtgatcaattatttgagtggtggagggctctgagggaagttgctgtggttctga aagtggctgggcttgtttcagacactctcttctgtaacataaacctatggttttcatgga gctctttggttctgatgaattgaaccacatcataaggccaaatttcatacctgggattgc tgatgacatgctcaggcttgttggatcatttgcttaatgcatggtgacagtgaggctaaa ttcttagtcattgctttgattaggcccctaccaggcttggagtgtctgctgcttatgaca ggcagctgagcctaagaccccatgacagcacagatactgagtagacagggcaagccaata agcttgacttggtggagatggcaacccagaaaatagtcctgaatgctgctgctgctagtg gtgatggtggtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtggt ggcttacaagtgcaaaaagaaagtaggatgtgtaaacttctcgtatgcatttctgaattc tttgagtgggtactggaaaagatgcaggagaccatgactgagagcctgggctaagagaac agctcagtagcttagggttggtggtggaagaatcttttaaatggctcctgaaccttttcc cacgagtcaggggaaaaaccaaactaaaccaaacccccccccccccaaaaaaaacccaaa caaacaaacaaacaaagaaaaacacaaggagatattctactgagaattgcacttgtagat ttcaatgtgtggattttgaagcactcatgaaacacaatctaaatcttaaatagtgaaagc attttcatgtgagctacagtaataaagttaacacttcaggcaggtgggacccattggcac cgggaataaaggtgctaggtggatatggcttttcggtgggctacctgtttgtacaggtag ggtgggagccagtttatttctttgccagacatcttaagtactgtagcttgtacttcccta gaggtaacaaggtaaaggttaacgtgcaggagggttattaggtgtgcctctgagatctac acttggggaagggaggaagagcagtatgttaacaggcaagttcaagatgcaatagagttg ccaggccactgttgctggtatatatgaagtatataccacatttcattcctcccttcatca gtggaagcaatccattgtcatggtgtttgctgtacatggagcaaagccagacactgttct gagtcttagaggttgagatctgagtcagacagatgacatccctatagtctaggaggagcc gggacttctaagtggaagatgataaataagacagaggcctcagctccaaaatgatcaaat aatatgatggctggaaaaatattggtggggcagtaactgactgatggctagagatatcag gagtcagaggcaggggaggacttctgagtggaacatagagcacccaggaacaaagggagt gtgttccagatcagaagcagagagtgggtgcatagagtgagtaaaggtgaaaggagacag gaccaaggccaggagtttgggctatttctaggaatgatgagagttgttagggaatttgaa agcaggcaggagccttctggcaaaggcatttcttagacagggtttaggacatgctcatcc agtgttttgaatattttatgtaattaatttaatccctgataatcctacctagaattcagt cttcgtctctcgcatttatagatgaggtaaccaaggcactgggagttttcttaatgacta ttctgagaccacagaagggagctctgggatctacagaggaggtctggctcactgttgttt ccttctgccccctaccccctgatgtgtgtacaaagatagcccaggtaagacttgaggaac acagcaccagtcaggagtctgtgacagtgggttctgtcctggaaataggtagaagatggg cacaggctgcggtgccgaggtgcagtttgattatgtggtgggcaaggaacacgaaagaag aatagaaggcaagtcaggttcttgtcatgaacaaatatctaaaatgaacataaatataat acagtggatcatacatgtttttcattcaagagtgatataggattgtagagaaattgttca gttatcagacatcctttagtgacctggaggaaaaactaaactaacttttctgaaggaata tggatgcctagccaggagtctgtttgatgataattgttgtgttgttagggaatttgagag caagcaggaaccttctagaaaggcttctttcccctcccacccagaaatccttccttctcc aagactagaatggcctgaacccttgctgtggtctgggtggggtaagatagagtaatgaca aggtgcctcaggcctcttcaccaccccctccccaaacagcagcctgtacagaaagctaat tagcatttagcctgttgccgcctgttctgatgctgcttgccagcacacacttagaggcag ttctatagactggcaccgtaaatacggaggtgttcacaccgtacaagatagaaatgctga tgttcagcagggatttggttagaccgtttcccaaagcattaatccctatacagagagcat agtcttttcattacactggagcttcaatccaccactgaaagaaagttggttcctagtgct agaggtaggggaggaagtatggcctcctttggagaaactccttgtgaagaattatggtaa ggaacatgtctttacaaagcccgctgttattattttaacccaaggattatgtagcagaaa attggggactatttgaatagttttaggtggaaaaagaagaaaagaaaattaatgtctagt taagtttgatttccccaccttttttttttcccttaaggctgactgtccaacttgctaatc attagccagattttgctacatattatggtcaaatttaaataaattaagagtgcatcagtg aaatactagtcatattagctggttcagaagaactcacttcctctctatgtggcttctgag tggagtcagttgtaggttgtgttcagagcgtataatctgagatgtctgaaatcttgaaag tgtgtgcctcagtgacctagggttgaaagggtttgtgcagaaaggtttgccgatggatct gaacacgagaacatgttacttcatctgtaaagctaattctgtaacctgtaggctggaacc tggcccaatccagaggtaaaataacaattcctggaacgctgtgtacgcggtcatttttct agctggttagtgcttgtttttgttgattaagatgtgtggtactgttttctaagcactttt acatgagcctttctctgctgcagattggtacagtcatctcggaggaatgcagtgtggggt gacggaatggttttattatacttaaaaatcctggtgaaagatatctacagtgtcgtgctg ggagagccgtatgagctggtgcttggaaagtttgcttagacttggagtttagaTCCTGTT CCCGTTAAGATCTGTPrimer
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